Droplets for cell encapsulation

Cell encapsulation pack

The Complete system for single-cell based assays

Ideal for single-cell analysis

From biochemical to omics analysis

Reproducible cell encapsulation

High accuracy and control for reproducible results

High precision and flexibility

From droplet generation to single cell encapsulation

Talk to an expert

Features & benefits

This pack is adaptable to several applications:

Oncology – mutations carried by small subpopulations of cells
Immunology – characterization of rare immune cell populations
Neurology – detailed single-cell mapping of different types of neurons and their connecting molecules
Developmental biology – mapping developmental processes of an embryo on a cell-by-cell basis
Single-cell omics – uncover the cellular heterogeneity of a tissue sample
And many more possible applications

Particle encapsulation help preserve the chemical, physical, and biological properties of active compounds – from drugs to nucleic acids, cells, and organoids.

Encapsulating cells in droplets:

create isolated cell culture chambers that speed up and optimize reaction times.
provides a physically and chemically isolated environment protected from cross-contamination.
is ideal for single-cell encapsulation for several cell-based assays – from microenvironment analysis to drug screening.

An all-in-one pack to generate droplets & encapsulate cells straight out of the box

Start from scratch and quickly master all key parameters – from droplet generation to cell encapsulation.

Whether you are an expert or a beginner, we provide all you need to understand droplet generation and start to encapsulate cells straight away.

This pack is composed of the premium Elveflow product line and our best-seller, the OB1 flow controller. Thanks to the high performance of this equipment set, you will be able to:

produce robust and highly monodisperse emulsions (CV<3%)
Fast and efficient mixing of reagents for droplet generation
have access to all the necessary elements to encapsulate cells easily
provide ultra-precise pressure-driven flow control
generate 10 to 80 µm diameter droplets (and more depending on your microchip)

All the pack items are adjustable to your laboratory infrastructure and experimental requirements.

Build your pack in three quick and easy steps:

Talk to our experts in encapsulation
Tell them what you want to do
Our experts will design a pack tailored to your needs

Benefit from the highest standards of microfluidic instruments

Microfluidics provides reproducibility and control over droplet size that you can not achieve using any other technique. Our technology allows you to:

handle ultra-small volumes to precisely control droplet generation.
easily generate identical droplets with the same content
create individual droplets with unique payloads.

droplet generation instuments sketch e1638442885466

Particle encapsulation help preserve the chemical, physical, and biological properties of active compounds – from drugs to nucleic acids, cells, and organoids.

Oncology – mutations carried by small subpopulations of cells
Immunology – characterization of rare immune cell populations
Neurology – detailed single-cell mapping of different types of neurons and their connecting molecules
Developmental biology – mapping developmental processes of an embryo on a cell-by-cell basis
Single-cell omics – uncover the cellular heterogeneity of a tissue sample
And many more possible applications

Application notes

- Emulsions generation & stability study
- Synthetic Organelles
- Nanoparticles & nanohydrogels
- Microgels
- Microbubble generation
- Liquid foam templating
- Single Cell Encapsulation
- Encapsulation
- Drug-delivery
- Microfluidic Droplets on demand
- Alginate beads production
- Droplets in microfluidics capillary
- Microfluidic flow focusing droplet generation

All our application notes

Reviews

- Introduction to droplet-based microfluidics
- Digital microfluidics using pressure driven flow
- Digital microfluidics: microfluidic droplets & emulsion science
- What is digital microfluidics?
- Researchers’ opinion on droplet generation in microfluidics: syringe pumps or pressure control?
- Droplet Sequencing (Drop-Seq)
- Droplets encapsulation for biological applications: a review
- Droplet detection and measurement in microfluidic channels
- Droplet production methods
- Sodium Alginate and applications: a review

All our reviews

Publications using our equipments

Image-based closed-loop feedback for highly mono-dispersed microdroplet production, Crawford et al, Scientific reports (2017)
High magnetic field gradient particle separation for biomedical applications. Conference Poster, Lennart Göpfert (2021).
Droplet microfluidics as a tool for production of bioactive calcium phosphate microparticles with controllable physicochemical properties, P. Habibovic et al., Acta Biomaterialia (2021).
3D flow-focusing microfluidic biofabrication: One-chip-fits-all hydrogel fiber architectures, Rui L. Reis et al. , Applied Materials Today (2021).
Thin film drainage dynamics of wheat and rye dough liquors and oat batter liquor, F. Janssen et al., Food Hydrocolloids (2021).
Coalescence Dynamics in Oil-In-Water Emulsions at Elevated Temperatures, B. Bera et al., Scientific Reports (2021)
Size-dependent particle migration and trapping in three-dimensional microbubble streaming flows Andreas Volk et al., Physical Review Fluids (2020).
High-Throughput Step Emulsification for the Production of Functional Materials Using a Glass Microfluidic Device Macromolecular Journals, D. Weitz et al.
Controllable generation and encapsulation of alginate fibers using droplet-based microfluidics Lab on a Chip, Nov 2015, A. J. deMello et al.
Image-based closed-loop feedback for highly mono-dispersed microdroplet production Scientific Reports, Sept 2017, G. Whyte et al.
Negative Pressure Induced Droplet Generation in a Microfluidic Flow-focusing Device Analytical Chemistry, Feb 2017, Say Hwa Tan et al

CELL ENCAPSULATION PACK CONTENT

1) 2-channel flow controller (OB1-2200)

2) 2 x flow rate sensors

- SMALL version: 1 x MFS-D-2 (aqueous phase) & 1 x 2 x MFS-D-3 (oil phase)
- LARGE version: 2 x MFS-D-2 (aqueous / oil phase)

3) 2 x flow resistors

- SMALL version: 1 x KFR-22-H (aqueous phase) & 1 x KFR-23-I (oil phase)
- LARGE version: 1 x KFR-22-H (aqueous phase) & 1 x KFR-22-I (oil phase)

4) 2 x ChipShop microfluidic droplet chips

5) Reservoirs and holder

- 6 x reservoirs with their pressurized caps (2 x 50mL; 2 x 15 mL; 2 x 1.5 mL)
- 1 x quick connect reservoir holder (WallE holder)

And more!

All necessary accessories: tubing, connectors, filters, etc…
1 x 10 mL vial of droplet oil (HFE-7500)
control and automation software (Elveflow Smart Interface)
1 x User Guide which includes:
- Detailed contents list
- Step-by-step installation guide
- Detailed protocol to generate droplets with the Pack
- Screenshots and pictures to get familiar with our equipment.

TWO VERSIONS FOR “SMALL” OR “LARGE” DROPLETS

Our two versions only differ by the flow rate range of the oil phase.

The SMALL version includes a MFS-D-3 allowing perfect feedback loop control of the flow rate to generate droplets ranging from 10 to 40 µm diameter.
The LARGE version includes a MFS-D-2 allowing perfect feedback loop control of the flow rate to generate droplets ranging from 50 to 80 µm diameter.

Important note: our products are all intercompatible and you may acquire additional flow rate sensors or add extra products for specific applications. You will still benefit from the centralised ESI software control.

Droplet-based microfluidics

Droplet-based webinars

Droplet-based applications

Testimonials

“I found the systems quite robust, easy to connect and use.”
Dr. Martino Chiara, ETH Zurich, Switzerland
OB1 pressure-driven flow controller user
“Thanks to Elveflow products, we can focus on our results rather than the tedious instrumentation. We especially appreciate the Elveflow system when it comes to droplet based systems which requires the control of multiple flows simultaneously. ”
Dr. Caglar Elbuken, Bilkent University, Turkey
OB1 pressure-driven flow controller user
“We are satisfied with the ELVEFLOW instrument, the regulation accuracy is well fitted with our applications. The advantage of this instrument is the capability it offers to easily move the experiments anywhere you want. This feature is very convenient, especially when we encapsulate cells into droplets in cell culture platforms.”
Pr. Annie Viallat, Adhesion & Inflammation Lab CNRS UMR 6212 Inserm UMR 600, France
OB1 pressure-driven flow controller user
“We are very satisfied of the ELVEFLOW pressure pumps! The ELVEFLOW pressure pumps enable us to perform portable experiments with accurate pressure control.”
Dr. Wiebcke Drenckhan, Liquids Interfaces Group of the Physic of Solids Lab - CNRS UMR 8502 Orsay University, France
OB1 pressure-driven flow controller user
“The advantages of this instrument are its ease of use and the accuracy of the regulated pressure. This feature is very convenient, especially when we perform foams and microfluidic.”
Dr. Pascal Panizza, Soft Matter Department of IPR – CNRS UR1 UMR6251, France
OB1 pressure-driven flow controller user
“I was suprised how quickly I was able to get things to work once I got going.”
Jamie Stover, Research Assistant, M. Zernicka-Goetz Lab at California Institute of Technology (Caltech)
OB1 pressure-driven flow controller user

Do you know how to implement droplet-based microfluidics?

[TS-VCSC-Youtube content_youtube=”https://www.youtube.com/watch?v=73EVh2mwSnk”]

[TS-VCSC-Youtube content_youtube=”https://www.youtube.com/watch?v=xXJ8K1DnVqc&t=3s”]

Are you aware of how to compute shear stress and microfluidic resistance in your system?

Elveflow developped a microfluidic calculator that allows you to estimate all the key parameters at stake in your microfluidic system. Find out more below !

To help you determine your flow rate, pressure to apply, the best tubing resistance length for your setup, wall shear stress for biology applications, cell culture, and many more…

Elveflow provides its microfluidic calculator and…to make the most of our microfluidic calculator, find below a set of dedicated application notes:

More details about the droplet pack

A well-chosen microfluidic chip

The microfluidic chip is where the two immiscible phases (water and oil here) are precisely injected and mixed to generate monodisperse droplets (CV < 3%). The two microfluidic chips are made of Topas. Topas is a cyclic olefin copolymer (COC) resin which is a chemical relative of polyethylene and other polyolefin plastics.

A reservoir holder with 2 push-in connectors

Are you working with small samples or are you looking into manufacturing 100x mLs of emulsion? Elveflow offers a comprehensive range of reservoirs compatible with our OB1 Flow Controller, from 1.5 mL Eppendorf tubes to 100mL bottles.

droplet generation monodispersed holder wall e

Enhanced flow resistor systems

Flow resistors consist in the assembly of PEEK capillaries with small internal diameters. Those resistors are used to increase the resistivity of the microfluidic system to improve the stability and control of the flow rate in the system.

02 Droplet pack Flow resistor 1536x949 1

A complete user guide

Simple and intuitive instructions are provided to quickly and easily make droplets and control droplet generation parameters. When starting out, the user can follow step by step the provided protocol to obtain droplets of the specific size. In a second stage, the user can rely on the numerous tips provided and explore the “going further” section to complete its training in droplet generation and microfluidic flow control.

Contact our experts to get yours!

Need help or technical information?

Talk to an expert

Products and associated accessories

OB1 MK4

Microfluidic Flow Controller

1 to 4 channels pressure & vacuum microfluidic flow controller

Microfluidic flow sensor - MFS

Microfluidic flow sensor

Thermal flow rate sensor

Bronkhorst flow sensor - BFS

Premium Coriolis flow sensor

The Rolls-royce of flow sensors

RESERVOIRS

MICROFLUIDIC RESERVOIRS

Need help or advice?

For any help to determine what microfluidic instruments you need, you can contact us!
Our experts will help you build the best microfluidic setup for your application, with our state-of-the-art microfluidic line.