Microfluidic application notes
We provide free microfluidic tutorials.
Browse through our numerous microfluidic Application Notes made by researchers for researchers to build your microfluidic setup.
These are detailed how-to tutorials based on several microfluidics applications in scientific research.
We help you with droplet generation and manipulation, avoiding air bubbles in microfluidics setups, performing live cell assays on microfluidics chips, how to set your flow regulation parameters and more. While you’re here, you might want to look at our free microfluidics resources:
- Open-access Research Summaries to keep you updated with the latest research in the field of microfluidics.
- Open-access Reviews for a broader understanding of microfluidic instruments and applications.
- Open-access microfluidic videos, images, and artistic pictures Just because we can.
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Real-Time Fluorescence Analysis of Mixing in a Tesla Micromixer
Real-Time Fluorescence Analysis of Mixing in a Tesla Micromixer
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Mechanoactivation of Single Stem Cells in Microgels Using a 3D-Printed Stimulation Device
A microfluidic and pressure-driven platform enabling reproducible mechanoactivation of individually encapsulated stem cells under live-cell imaging in a true 3D environment.
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High-Resolution Microfluidic Device for Precise Oil-in-Water Emulsion and Coalescence Analysis
Discover a high-resolution microfluidic device designed for precise oil-in-water emulsion studies and coalescence analysis. Learn how Elveflow's advanced flow control enhances petroleum research and beyond.
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Water-In-Oil Droplet Generation under Microscopy Monitoring
Discover a simple and intuitive microfluidic water-in-oil droplet protocol under microscope monitoring
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Direct-Ink-Writing Flow Control with Elveflow Pressure Sensor
This application note demonstrates a smart use ouf Elveflow's Pressure sensor and sensor reader for Direct-Ink-Writing flow control.
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Elveflow SDK Tutorial Part 1: Setting Up Your Environment on the OB1
Learn how to set up your development environment for Elveflow products with this comprehensive tutorial.
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Utilizing a high precision microfluidic dead-end filtration setup to achieve global alignment of single walled carbon nanotubes
Single-wall carbon nanotubes (SWCNTs) are considered as quasi 1-dimensional (1D) carbon nanostructures, which are known for their outstanding anisotropic electronic, mechanical, thermal and optical properties.
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How do you synchronize a microscope with a microfluidic perfusion system?
This application note describes how to combine and synchronise liquid perfusion and imaging using an Olympus spinning disc confocal microscope together with an Elveflow pressure-driven flow controlled microfluidic system.
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REDIRECTING FLOW WITH A MICROFLUIDIC VALVE CONTROLLER
The MUX Wire enables you to host up & control many valves of your choice. It gives you a total freedom to add up to 16 valves and put them anywhere in your set-up and control them independently and simultaneously.
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Active Micromixers-on-a-Chip
Mixing is a crucial step for several microfluidic applications like chemical synthesis, clinical diagnostics, sequencing and synthesis of nucleic acids
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Nanoparticle generator
This application note describes how microfluidic can be employed as a nanoparticle generator based on the example of PLGA bead generation.
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Microfluidic colocalization set-up for DNA analysis
This user guide will show you how to run microfluidic colocalization studies of single molecule spectroscopy.
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Exploring Pneumatic Pumps and Pressure-Driven Flow Control Technology
This application note explores the basic principle of pneumatic pumps and a flow controller based on the basic principle of pneumatic pumps, known as pressure driven flow control. It also demonstrates the applications of pressure driven flow control in a range of industrial & research fields.
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Setup: microfluidic flow control
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Nanobubble generation
Nanobubble generation performed by microfluidics is described in an application note. Nanobubbles are very singular by their generation and properties.
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How to handle monodisperse alginate droplets & beads production with microfluidics
Highly monodispersed alginate beads can be easily generated with a microfluidic droplet generation system.
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Sequential Production and Trapping of Droplets
Microfluidic sequential production and trapping of droplets for in situ optical analysis.
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Droplets generation & manipulation
The generation and manipulation of droplets through microfluidics offer tremendous advantages: better control over small volumes of fluid, enhanced mixing, high throughput experiments.
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Double emulsion for encapsulation using microfluidics
A protocol for high monodispersity and high encapsulation efficiency double emulsion production for encapsulation using microfluidics
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Antifungal screening on chip using droplet based microfluidics
Droplet-based microfluidics and single spore encapsulation offer the key for a breakthrough in antifungal screening and fungicide discovery.
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Single cell encapsulation
Generate picoliter-sized microdroplets and perform single cell encapsulation with this detailled microfluidic protocol.
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Microbubble generation
Everything you need to know about microbubble generation, from theory to the microfluidics experimental steps.
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How to perform single spore encapsulation
This application note will show you how highly monodispersed droplets can be easily generated to encapsulate single spore of fungus using a microfluidic droplet generation system.
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Droplets on demand in microfluidic networks
Two-phase flow microfluidics allow to perfom different laboratory functions on one microfluidic Lab-on-Chip (LoC) platform. An example of such an application is the controlled production of droplets on chip.
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Droplet generation inside a capillary
This application note describes how to generate controlled size millifluidic droplets in a capillary by regulating the pressure rate and/or by regulating the flow rate.
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Digital microfluidics: droplet generation inside a microfluidic chip
Droplets generation has a large scale of applications, such as emulsion production, single cell analysis, drug delivery or nanoparticles synthesis. Droplets can also be used as micro bioreactors for chemical or biochemical reactions.
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Microfluidic Flow focusing droplet generation
Precise and effective control of droplet generation is critical for applications of droplet microfluidics ranging from materials synthesis to lab-on-a-chip systems.
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Microfluidic Droplets on demand
Active droplet generation in microfluidics is of high interest for a wide range of applications. It provides an additional degree of freedom in manipulating both the size and the formation frequency of micro-droplets. This additional control is extremely desirable for complex operations which rely on the accurate control of both parameters.
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Setup: droplet and digital microfluidics
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Biofilm testing under flow
Biofilm testing using a simple microfluidic chip channel for in situ observation of their development under flow conditions.
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Microfluidics for microscopy imaging and treatment of Arabidopsis thaliana primary roots in vivo
Microfluidics for microscopy imaging in plant biology allows to observe, in vivo, the biological response of plant roots to various stimuli.
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Automated Cell-Seeding for Dynamic Cell Culture
A simple guide to do dynamic cell culture by automating cell seeding in a microlfuidic chip
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Cell-cell interactions on chip
This application note describes how co-culture of different cell types in separate but interconnected chambers is possible in a microfluidic platform
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How to study bacteria by microfluidics
This application note explains how to study bacteria adaptation to stress and environmental changes such as antibiotics.
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Medium recirculation using microfluidic valves
In this application note we describe how to set up medium recirculation by using microfluidic valves
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Medium recirculation for dynamic cell culture
In this application note we describe how to create a medium recirculation for dynamic cell culture with a microfluidic setup.
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How to stain cells cultured in a microfluidic chip?
In this application note we describe how to stain cells for dynamic cell culture with different microfluidic setups.
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Microfluidic perfusion for dynamic cell culture
In this application note we describe how to do cell perfusion for dynamic cell culture and a way to enable uni-directional recirculation of medium.
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Microfluidic cardiac cell culture model
This application note proposes a microfluidic cardiac cell culture model (μCCCM) to recreate mechanical loading conditions observed in the native heart (in both normal and pathological conditions) by using an Elveflow OB1 pressure and flow controller.
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Using the perfusion pack to perform fast medium switch
In this application note, we will describe how to perform an automated and fast medium switch thanks to the Perfusion Pack.
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Microfluidic Perfusion medium switch using IBIDI© chips
Medium switch is widely used in cell biology. One application is the study of cell behavior under given flow conditions for different samples. In this tutorial, we walk you through the steps of a fast and stable medium switch using IBIDI© flow cells.
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Fluorescence reader for microfluidic qPCR
Fluorescence reader for microfluidic qPCR: faster, more sensitive and less expensive than most optical microscopes, it is a smart alternative for real-time fluorescence measurements of your on-chip qPCR signal.
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Circulating Tumor Cells entrapment using microfluidics
Prostate cancer is the second leading cause of cancer-related death for men. Circulating tumor cells (CTCs) are considered as a marker of early cancer diagnosis and disease severity. Their screening in blood is thus crucial to detect metastatic stage in cancer patients.
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Microfluidic cell culture
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