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Recirculation Pack

Microfluidic recirculation pack

Full system for continuous unidirectional recirculation experiments
Unidirectional liquid flow

The liquid stream flows continuously in the same direction

No more media depletion

Don't be limited by your sample volume: recirculate your medium

Uniform Shear Stress

Same flow direction ensures no variations in shear stress

Week-long experiments

Automate your experiments for several days

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A standard Recirculation Pack setup uses two pumping channels to alternatively flow medium from one reservoir to another. An injection valve allows to switch between both reservoirs, to ensure that the medium is pumped from the most filled reservoir. The injection loop consists of a network of inputs/outputs that can be connected to each other in two configurations. Its very low internal volume (2.5 μL) makes it the ideal valve to perform the injection of a controlled volume of sample into a chip.

How to perform fluid recirculation?

The Elveflow® smart interface (ESI) synchronizes the flow controller together with the injection valve in order to perform recirculation. It performs the switch between reservoirs automatically either after a predetermined period of time or, in combination with a the flow rate sensor (MFS), after a chosen amount of sample has gone through the chip. The software is also able to seamlessly run long term experiments thanks to its powerful scheduler.

Additional features such as medium switch, one-off sample injection, etc… are also possible by adding extra Elveflow’s equipment to your Recirculation Pack.

 Contact us for further information!

Microfluidic calculator to estimate the shear stress in your cell culture chamber

To help you determine your flow rate, pressure to apply, the best tubing resistance length for your setup, wall shear stress for biology applications, cell culture, and many more…

Elveflow provides its microfluidic calculator and…to make the most of our microfluidic calculator, find below a set of dedicated application notes:

Publications

Adaptable pulsatile flow generated from stem cell-derived cardiomyocytes using quantitative imaging-based signal transduction

T.  Qian et. al, Lab on a Chip, 2020, 20, 3744-3756, DOI: 10.1039/D0LC00546K

Investigation of flow dynamics and structure in channels with structured surfaces

P. Vilkinis, 2020, Doctoral Thesis, Kaunas University of Technology, Lituanian Energy Institute

CONTENT

A standard Recirculation Pack contains the following:

OPTIONS

  • Microfluidic cell culture chips: Elveflow is working with several partners who can provide chips specifically designed for certain microfluidic cell culture experiments. If you are interested, please contact us at contact@elveflow.com
  • Bubble Trap: add it to remove bubbles from your microfluidic setup
  • Additional reservoirs
  • Additional flow rate sensors (consult the MFS and BFS sensor product page for more specification details)
  • Computer
  • Microscope and camera

Videos

Microfluidics related webinars

colored bubble

Testimonials

Need help or technical information?
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Need help or advice?

For any help to determine what microfluidic instruments you need, you can contact us!
Our experts will help you build the best microfluidic setup for your application, with our state-of-the-art microfluidic line.

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