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Sequential sample Injection

Liquid handling pack

Automate switching between up to 12 liquid samples
MULTIPLE SAMPLE INJECTION

Quickly swap between up to 12 liquid samples

HIGH STABILITY AND PRECISION

Flow can be tuned to match any requirements (flow rate, injection volumes)

WORKFLOW AUTOMATION

No more time consuming experiment

CUSTOMIZATION

Use it with any liquid system

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Our liquid handling pack enables to sequentially inject different liquids.  It contains: a MUX Distribution bidirectional valve which works as a selector, a high accuracy flow controller, and Elveflow’s intuitive ESI control software. 

The pack also contains a manifold to pressurize several reservoirs using a single pressure channel from the OB1 and a flow sensor to monitor and control the flow rate in real-time.

The MUX distribution rotary valve is characterized by:

  • Valve flexibility: 12 inlets-1 outlet or 1 inlet-12 outlets
  • Easy setup and operation: plug and play software and standard fluidic connection 
  • Possibility to choose the sense of rotation
  • The versatility of the system in terms of fluids.

If your experiment requires the use of a buffer in large quantities but also expensive reagents in tiny volumes, it is not an issue for our liquid handling pack. You can actually connect tanks with various volumes, from bottle to Eppendorf.

Moreover, there is no restriction concerning the use of liquids as the wetted materials of the system are resistant to aggressive reagents. The flow sensors can also be chosen to work with liquids of different properties

Advantages

  • Quickly swap your biological media or chemical solutions and inject them thanks to the MUX Distribution valve
  • Flow can be tuned to match any requirements (pressure, flow rate, injected volumes)
  • Automation of the whole system
  • Enables both flow and pressure control when used with a flow sensor
  • Possible to handle both small (µL) and large volumes of liquids (Liters)
  • Multiple parameters control (volumes, time of injection…) 
  • Various options for sample collection
  • Works with any liquids (water, oil…)
  • Very large flow rate range depending on the flow sensor: from nL/min to mL/min
  • Automation and integration
  • Scale up: injection of numerous solutions (up to about 150 solutions)
  • Customization

How to perform microfluidic multiple liquid switching?

More information on how to perform a stable and precise flow switch in our example application note.

Our liquid handling pack can be adapted for more complex and advanced experiments such as using 20 or more solutions, removing bubbles, integration into larger systems or testing multiple chip/devices simultaneously…

Contact us to know more!

MUX DISTRIBUTION ELVEFLOW NEW

Complete Userguide | Automated Liquid Handling

To discover more tips and tricks…

Get in touch to claim our complete Userguide about Liquid handling & Sequential Injection!

Userguide Fluid Sequential Injection

The liquid handling pack can be used in various fields of application and for any experiment that require successive injection of liquids:

  • Flow chemistry / lab on chip / functionalization of sensors: The sequential injection is interesting in applications related to flow chemistry, lab on a chip, and functionalization of sensors.
  • Calibration of sensors / biosensors: The sequential injection makes it possible to automate and to reduce the duration of testing.
  • Droplets: The combination of sequential injection and droplets can make experiments in drug delivery, sensitivity tests, screening, microfluidic PCR and gradient assay easier.
Liquid handling pack - Droplet generation -
Brouzes et al, 2009

 

  • Cell biology / cell culture: Experiments such as cell response to medium changes, live cell imaging, drug testing, 3D cell culture and spatial transcriptomics that can be improved and easily automated.
Cell biology - cell culture - liquid handling pack
Sukenik et al, 2017

 

Application notes

  • How to control the flow while flow line changing with a Mux Distributor ?
  • How to perform fast drug or medium switch?
  • How to perform controlled drug switches with an Elveflow® MUX?
  • How to perform an ultrafast microfluidic medium switch with an Elveflow® pressure & flow control instrument (OB1)
  • How to perform the injection of a controlled volume of sample?
  • See all application notes

For more biology-related content, please refer to the cell perfusion application pack.

Reviews

  • Microfluidics as a tool for drug delivery
  • Electrochemistry and Microfluidics : A short review
  • Microreactors & Microfluidics in Chemistry: a Review
  • Microfluidic Nanoparticle Synthesis: A short review
  • See all reviews

Publications

Adaptable pulsatile flow generated from stem cell-derived cardiomyocytes using quantitative imaging-based signal transduction

T. Qian et al., Lab on a  Chip, 2020, 20, 3744-3756, DOI: 10.1039/D0LC00546K

Microfluidic platform for 3D cell culture with live imaging and clone retrieval

C. Mulas et al., Lab on a chip, 2020, 20, 2580-2591, DOI: 10.1039/D0LC00165A

The amazing benefits of microfluidics can be applied to almost any application and therefore the content of the liquid handling pack can be adjusted to suit your specific needs. Contact us for more details.

CONTENT

Selecting the best flow control method for your application is very important, since the performance and fluid handling efficiency of a microfluidic device depends highly on the flow control system. If you require to use our pack for the previously mentioned applications, the solution will contain:

  • Pressure & flow controller (OB1)
  • Tubing and connectors
  • Software and SDK libraries (C++, Python, MATLAB, LabVIEW)

OPTIONS

You can choose from the following additional options: 

  • Additional pumping channels
  • Microfluidic valves (2-way and 3-way valves)
  • Microfluidic chip
  • Computer
  • Manifold

Multiple Liquid switch videos

Related microfluidics webinars

colored bubble

Testimonials

Liquid handling & Sequential Fluid Injection Webinar

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Need help or technical information?
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Software

A free software for all instruments

Powerful, modular and versatile set-up control solution

The ESI software can control up to 16 Instruments through the same interface. Thanks to TTL triggers you can synchronize your Elveflow system with any other instrument used in your laboratory (microscope or any electrical instrument … ). The Scheduler is a user-friendly tool to automate complex steps of your experiments and protocols.

Read more

Volume
Injection
module

Enter your targeted volume and this module will automatically adjust the flow rate at the right time to inject it.

Fluidic system optimization module

Automatic diagnostic of the fluidic path sizing and recommendations to improve your microfluidic setup.

Bubble
detection
module

Do not suffer anymore because of bubbles!

Sensor calibration module

Don’t lose precious time in calibration protocols.

Need help or advice?

For any help to determine what microfluidic instruments you need, you can contact us!
Our experts will help you build the best microfluidic setup for your application, with our state-of-the-art microfluidic line.

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