Archived content. See more recent content: Application notes & reviews
During C. elegans embryo division, the CYK-4 protein is required for the completion of cytokinesis (but not to form a furrow or initiate ingression). At the “permissive” 16°C temperature, the CYK-4 protein is functional but over 25°C it becomes inactivated. Such a mutant is called “thermosensitive”.
Fig 1: CYK-4 ts C. elegans control of cytokinesis by ElveflowTemp. When temperature is set to 16°C, cell division goes on normally (a-f). Immediately after quick change of temperature (25°C) the CYK-4 protein is inactivated resulting in cytokinesis arrest.
During our experimentation, C. elegans thermosensitive embryo mutants (CYK-4 ts) were examined under the microscope with a ElveflowTemp platform. This system allowed to selectively modify the temperature of the embryo samples between 16°C and 25°C resulting in cytokinesis blocking. Fig 1 shows unprecedently observed phenomenon during cell division.
ElveflowTemp offers unique properties to get the best tuning of C.elegans samples temperature and obtain results that could not be observed otherwise!
Feel free to contact us at: contact@elveflow.com
Email*
Message Hi Elveflow team, I need more information about Newsletter subscription
Email* I hereby agree than Elveflow uses my personal data
Get a quote
Name*
Message
Newsletter subscription
We will answer within 24 hours
By filling in your info you accept that we use your data.
Collaborations
Need customer support?
Serial Number of your product
Support Type AdviceHardware SupportSoftware Support
Subject*
I hereby agree that Elveflow uses my personal data Newsletter subscription
How can we help you?
Message I hereby agree that Elveflow uses my personal data Newsletter subscription