Microfluidic application notes

Category:
Microfluidic cell culture

We made a bold choice to fit both our mission, which is to boost science using microfluidics, as well as our culture, which is to do things our way and to be part of the research community.

We decided to promote and sell our instruments, not through common, boring advertisement, but instead by providing you with:

Open access short reviews, to help researchers to train themselves in microfluidics
Open access application notes, to help researchers to quickly set up their microfluidic instruments for their applications
Open access microfluidic videos, images and artistic pictures… Just because we like it!

And then we hope that while surfing our website, some of you will discover our microfluidic brand and fall in love with Elveflow.

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Biofilm testing under flow

Biofilm testing using a simple microfluidic chip channel for in situ observation of their development under flow conditions.

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Microfluidics for microscopy imaging and treatment of Arabidopsis thaliana primary roots in vivo

Microfluidics for microscopy imaging in plant biology allows to observe, in vivo, the biological response of plant roots to various stimuli.

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Automated Cell-Seeding for Dynamic Cell Culture

A simple guide to do dynamic cell culture by automating cell seeding in a microlfuidic chip

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Cell-cell interactions on chip

This application note describes how co-culture of different cell types in separate but interconnected chambers is possible in a microfluidic platform

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How to study bacteria by microfluidics

This application note explains how to study bacteria adaptation to stress and environmental changes such as antibiotics.

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Medium recirculation using microfluidic valves

In this application note we describe how to set up medium recirculation by using microfluidic valves

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Medium recirculation for dynamic cell culture

In this application note we describe how to create a medium recirculation for dynamic cell culture with a microfluidic setup.

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How to stain cells cultured in a microfluidic chip?

In this application note we describe how to stain cells for dynamic cell culture with different microfluidic setups.

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Microfluidic perfusion for dynamic cell culture

In this application note we describe how to do cell perfusion for dynamic cell culture and a way to enable uni-directional recirculation of medium.

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Microfluidic cardiac cell culture model

This application note proposes a microfluidic cardiac cell culture model (μCCCM) to recreate mechanical loading conditions observed in the native heart (in both normal and pathological conditions) by using an Elveflow OB1 pressure and flow controller.

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Using the perfusion pack to perform fast medium switch

In this application note, we will describe how to perform an automated and fast medium switch thanks to the Perfusion Pack.

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Microfluidic Perfusion medium switch using IBIDI© chips

Medium switch is widely used in cell biology. One application is the study of cell behavior under given flow conditions for different samples. In this tutorial, we walk you through the steps of a fast and stable medium switch using IBIDI© flow cells.

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Fluorescence reader for microfluidic qPCR

Fluorescence reader for microfluidic qPCR: faster, more sensitive and less expensive than most optical microscopes, it is a smart alternative for real-time fluorescence measurements of your on-chip qPCR signal.

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Circulating Tumor Cells entrapment using microfluidics

Prostate cancer is the second leading cause of cancer-related death for men. Circulating tumor cells (CTCs) are considered as a marker of early cancer diagnosis and disease severity. Their screening in blood is thus crucial to detect metastatic stage in cancer patients.

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Tissue engineering: Heart Ventricule assisted by a microfluidic pressure pump

Until recently, microfluidic devices have been employed to support tissue-engineering experiments on basal lamina, vascular tissue, liver, bone, cartilage and neurons as well as organ-on-chips.

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Performing live cells assays on chip

This application note presents how to perform cell culture on chip using the Cell and Biology Pack and the MicroSlides developed by ALine Inc.

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Stimulation of C. elegans mechanoreceptor neurons using microfluidics [Publication Recap]

This article covers the work of Adam L. Nekimken et al. published in Lab Chip in January 2017. The research team introduced a novel microfluidic device that enables high-resolution imaging of cellular deformations in response to precise mechanical stimuli to the surface of the C. Elegans worm cuticle.

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Weak protein interactions quantified using a microfluidic pump [Publication recap]

This article covers the development of an experimental method enabling Shahar Sukenik et al. to study protein interactions and detect the dissociation of GAPDH and PGK proteins in order to quantify their stoichiometry directly inside the cell by modulating the cell-volume

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Cell biology

Microfluidics is widely used to develop tools for cell biology. The micrometer scale of microfluidic devices is particularly adapted to work with cells.

Category:
Microfluidic cell culture

Biofilm testing under flow

Microfluidics for microscopy imaging and treatment of Arabidopsis thaliana primary roots in vivo

Automated Cell-Seeding for Dynamic Cell Culture

Cell-cell interactions on chip

How to study bacteria by microfluidics

Medium recirculation using microfluidic valves

Medium recirculation for dynamic cell culture

How to stain cells cultured in a microfluidic chip?

Microfluidic perfusion for dynamic cell culture

Microfluidic cardiac cell culture model

Using the perfusion pack to perform fast medium switch

Microfluidic Perfusion medium switch using IBIDI© chips

Fluorescence reader for microfluidic qPCR

Circulating Tumor Cells entrapment using microfluidics

Tissue engineering: Heart Ventricule assisted by a microfluidic pressure pump

Performing live cells assays on chip

Stimulation of C. elegans mechanoreceptor neurons using microfluidics [Publication Recap]

Weak protein interactions quantified using a microfluidic pump [Publication recap]

Cell biology

For more information or Technical discussion

For more information or Technical discussion

For more information or Technical discussion

For more information or Technical discussion

Category:Microfluidic cell culture

Biofilm testing under flow

Microfluidics for microscopy imaging and treatment of Arabidopsis thaliana primary roots in vivo

Automated Cell-Seeding for Dynamic Cell Culture

Cell-cell interactions on chip

How to study bacteria by microfluidics

Medium recirculation using microfluidic valves

Medium recirculation for dynamic cell culture

How to stain cells cultured in a microfluidic chip?

Microfluidic perfusion for dynamic cell culture

Microfluidic cardiac cell culture model

Using the perfusion pack to perform fast medium switch

Microfluidic Perfusion medium switch using IBIDI© chips

Fluorescence reader for microfluidic qPCR

Circulating Tumor Cells entrapment using microfluidics

Tissue engineering: Heart Ventricule assisted by a microfluidic pressure pump

Performing live cells assays on chip

Stimulation of C. elegans mechanoreceptor neurons using microfluidics [Publication Recap]

Weak protein interactions quantified using a microfluidic pump [Publication recap]

Cell biology

For more information or Technical discussion

For more information or Technical discussion

For more information or Technical discussion

Category:
Microfluidic cell culture