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Real time droplet generation with flow focusing geometry of the glass microfluidic droplet generation pack.
This microfluidic droplet chip application note describes how to generate droplets by microfluidics and is realized with a glass microfluidic droplet generation pack. This note details how to generate controlled size microfluidic droplets in a glass microfluidic chip by regulating the pressure rate and/or by regulating the flow rate. Both T-junction and flow focusing geometries are used for droplet generation.
The accuracy of piezoelectric technology supporting the performance of the pressure-driven flow controller employed in this study (OB1 MkIII pressure controller) allows for an effective pressure regulation, high flow accuracy and fast ms response time.
Droplet generation by microfluidic droplet chip has a large scale of applications, such as emulsion production, single cell analysis, drug delivery, polymer beads or nanoparticles synthesis. You might want to use droplets as micro bioreactors for chemical or biochemical reactions.
These are the key features to watch out for to ensure successful droplet generation:
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Flow controller
Microfluidic Droplet Pack
Flow sensor
Reservoirs
Tubing
Fittings
Using the Elveflow® smart interface to generate controlled size droplets:
The following diagrams illustrate the complete chain of elements involved in this application note. You will need to assemble all these parts to set up your experiment and generate controlled size droplets.
Glass enables to overcome some of the drawbacks of Polydimethilsiloxane (PDMS), such as its porosity and solvents adsorption. More insight into PDMS chips & Soft lithography available here.
A) Monitoring the pressure to generate controlled size droplets:
The Elveflow® smart interface allows to control pressure for oil and water samples.
One can also program sequences in order to generate droplet streams, oil plugs of controlled sized etc.
B) Monitoring the flow rate to generate controlled size droplets:
When coupled with Flow Sensors our controller enables to control the flow rate thanks to a PID feedback loop. One can request the flow values in the Elveflow® smart interface and the pressure controller will adjust the pressure consequently to reach the requested values.
Congratulations! You’ve achieved the generation of controlled-size droplets with glass microfluidic droplet pack using an Elveflow® Flow sensor and the Elveflow® pressure control instrument!
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Do you want tips on how to best set up your microfluidic experiment? Do you need inspiration or a different angle to take on your specific problem? Well, we probably have an application note just for you, feel free to check them out!
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