Archived content. See more recent content: Application notes & reviews
3D cell culture is a powerful tool to mimic more closely the in vivo environment of the cells. For example, cancerous cells can be seeded in matrigel and form multicellular spheroids. See how our Perfusion Pack can help you design and achieve groundbreaking experiments.
With the Perfusion Pack, you can easily switch between 2 perfusion medium and thus change the composition of the cells environment.
3D cell culture in Matrigel can be tricky. With the Perfusion Pack, you have a perfect control on the flow rate you apply. You can perfuse your cells with very low flow rates (down to 0.1 μL/min) and make sure that your cell culture never suffers from shear stress.
Confocal microscopy is the best way to obtain 3D images of your spheroids. Contrary to syringe pumps, our Perfusion Pack provides a pulseless flow, avoiding any movements inside your perfusion chamber that can interfere with your confocal microscope focus.
Technical review about perfusion systems for live-cell imaging
General review about perfusion chambers
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