This scenario revolves around developing the TAMARA nanoparticle formulation system, a microfluidic-based system tailored for researchers seeking simplicity, precision, and reproducibility in lipid and polymeric nanoparticle synthesis, such as LNP, liposomes and PLGA nanoparticles. The integration of Elveflow’s OEM controller serves as the platform’s embedded flow control system.
Written by Robin Oliveres, CBO, Inside Therapeutics
At the convergence of microfluidics and biotechnology, Inside Therapeutics strives to provide laboratories, biotech, and pharma companies with the ultimate nanoparticle synthesis capabilities to unlock the full potential of RNA-LNP therapeutics and nanomedicinesTAMARA is a microfluidic-based platform designed for researchers who seek ease of use, control, and repeatability in the synthesis of nanoparticles.
Inside sought a system capable of precise control over very low volumes, with zero losses, while offering controllable and stable flow control for optimal control of the nanoparticle’s physicochemical parameters (size, PDI, encapsulation efficiency). It was clear that the integration of Elveflow’s pressure-driven flow control was the definitive choice to meet these expectations.
“With the versatile capabilities of the OB1 pressure controller, we were able to design a system capable of precisely managing tiny volumes of liquids (as small as 50µL) while upholding optimal control overflow conditions, crucial for effective nanoparticle formulation. Moreover, the hardware provided exhibited excellent quality, facilitating the seamless completion of all our quality certifications.”
Matthieu Kerhuel, CTO
The system comprises two components:
A controller, which embeds a touchscreen used as the graphic user interface. It is paired with the OB1 pressure controller in the OEM version, ensuring precise pressure delivery to the synthesis module. The adoption of Elveflow’s OB1 OEM was evident, as it offers a unique trade-off between rapid response time, ensuring 100% sample injection with no losses, and excellent stability and repeatability for very homogeneous nanoparticle formulation.
The synthesis module is a comprehensive unit housing a casing, liquid reservoir, and a microfluidic chip where the two liquids mix, triggering nanoparticle synthesis.
These two components are linked by a pneumatic cable, resulting in a tubing-free final system.
Within the controller, the OB1 OEM takes center stage as users input their experimental parameters, encompassing the total flow rate (TFR), flow rate ratio (FRR), and total volume. Already having an in-depth knowledge of the chip’s characteristics, the target flow rates are converted into precise pressure values. Subsequently, these pressure values are applied to the reservoirs integrated into the synthesis modules, facilitating the controlled injection of fluids at the desired TFR and FRR within the system. This precise control over synthesis conditions allows users to effectively manage the final physicochemical attributes of Lipid and polymeric nanoparticles, such as Size, PDI, Zeta potential, and Encapsulation Efficiency..
Fig. Results achieved with TAMARA PlatformSource: TAMARA Introduction
Given the high cost of RNA and thus the necessity to use even tiny quantities of liquid, TAMARA has been engineered to efficiently handle a wide range of volumes, from as little as 200µL to over 5mL with no losses. This was made possible thanks to the OB1’s excellent response time. This unique versatility ensures that users can rely on the same formulation platform from initial screening to in-vivo testing, maintaining high performance throughout their drug development.
Moreover, the non-contact nature of the pressure-driven flow control plays a pivotal role in preventing cross-contamination in TAMARA , a crucial aspect in pharmaceutical development. This feature also simplifies the cleaning process, making the system more user-friendly and suitable for intricate pharmaceutical applications.
Recognizing that the primary end-users typically consist of biochemists and pharmacists lacking prior microfluidics experience but possessing stringent technical requirements, Inside Therapeutics aimed to create a platform that excels in nanoparticle formulation performance, even at very low volumes. The design prioritizes simplicity and effectiveness, ensuring optimal results for users regardless of their familiarity with microfluidics.
“Working with Elveflow’s equipment for the development of our nanoparticle synthesis platform was a natural decision. It not only accelerates our development process but also guarantees the utmost precision in microfluidic flow control, a pivotal aspect of our platform’s success in nanoparticle synthesis.”
Thomas Guerinier, CEO
The OEM version of the OB1 pressure controller from Elveflow, integrated into the platform, makes a major contribution to its quality through its intrinsic performances. TAMARA is the perfect companion for any nanoparticle specialist – from beginners to experts – looking for a comprehensive, user-friendly, and efficient nanoparticle system for the development of novel drugs. With the help of Elveflow, Inside Therapeutics successfully achieved its target: developing a user-friendly system to provide nanoparticle specialists with access to the latest microfluidic technology across all the preclinical development stages.
Elveflow would like to thank Inside for the trust it has shown in us over the last few years and for the solid collaboration.
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