Overview of the screening method for microcolonies by Duarte, J. et al.
Avoid double emulsions (water-in-oil-in water)
For FACS-based sorting
Use our all-in-one setup for different samples and various containers
Analyse a large number of cells in a short amount of time
Fluorescence activated cell sorting (FACS) has a high level of flexibility, the ability to simultaneously screen multiple parameters, and an unmatched throughput of >107 events/hour. It sorts individual cells according to their fluorescence.
However, in synthetic biology, FACS has not yet been widely used for screening libraries of synthetic circuits [1]. That’s why, microcolonies sorting make it possible to take advantage of the FACS technique without being reliant on single cell readouts. The microcolonies can be confined in hydrogel beads [2-4].
Whether you want to do microcolonies sorting or single cell screening, we offer you an all-in-one setup to prepare your sample for FACS.
The pack allows you to encapsulate cells in alginate beads.
An all-in-one pack guarantees a good compatibility between the different instruments, allows you to start your experiment right away, is piloted by a single software and can be used for other different applications. These are a few arguments why a pack is the easiest way to setup a microfluidic experiment.
Our setup allows you to entrap cells in alginate beads. Once incubated, they grow into microcolonies for fluorescence activated cell sorting.
You can also use the setup for single cell screening as well.
1. Davies, D. (2012) Cell separations by flow cytometry. Methods Mol. Biol. 878, 185−199.
2. Weaver, J. C., Bliss, J. G., Powell, K. T., Harrison, G. I., and Williams, G. B. (1991) Rapid clonal growth measurements at the single-cell level: gel microdroplets and flow cytometry. Nat. Biotechnol. 9, 873−877.
3. Sahar, E., Nir, R., and Lamed, R. (1994) Flow cytometric analysis of entire microbial colonies. Cytometry 15, 213−221.
4. Zengler, K., Toledo, G., Rappe, M., Elkins, J., Mathur, E. J., Short, J. M., and Keller, M. (2002) Cultivating the uncultured. Proc. Natl. Acad. Sci. U. S. A. 99, 15681−15686.
5. Duarte, J. M., Barbier, I., & Schaerli, Y. (2017). Bacterial microcolonies in gel beads for high-throughput screening of libraries in synthetic biology. ACS synthetic biology, 6(11), 1988-1995.
Microfluidic fluorescence-activated cell sorting (FACS) is a powerful tool used to isolate and sort specific cells from a heterogeneous population of cells.
It has a wide range of applications in various fields, including biology, medicine, and biotechnology.
Some applications include:
Overall, microfluidic FACS provides a powerful tool for cell analysis, sorting, and manipulation, which has a wide range of applications in various fields.
The size and frequency of the droplets will depend on the pressure, flow rate and viscosity of the liquids used to generate the alginate beads. See droplet size/pressure diagram below.
For any help to determine what microfluidic instruments you need, you can contact us! Our experts will help you build the best microfluidic setup for your application, with our state-of-the-art microfluidic line.
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