contact@elveflow.com+33(0).184.163.807+1(414)-406-4343 Elveflow is an Elvesys brand, an international microfluidics innovation center with high level researchers dedicated to microfluidics and organ-on-a-chip.
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Different kinds of cell cultures can be found nowadays, and some would be more suited than others depending on its properties and applications. ) [insert] => ooc2 [utiliser_acf] => [most_popular] => [review_type] => 13 [citation_author_repeater] => [citation_publication_date] => 2023/08/14 [citation_keywords_repeater] => [description_GS] => [header__content] => Array ( [image__header] => [subtitle_text__header] => [title__header_ad] => [content__text__ad] => [link__header] => ) [header__content___sidebar_ads2] => Array ( [image__header_ads2] => [subtitle_text__header_ads2] => [title__header_ad_ads2] => [sub_title_2_ads2] => [content__text__ad_ads2] => [link__header_ads2] => ) [ID] => 3673 [title] => 3D cell culture methods and applications [permalink] => https://www.elveflow.com/microfluidic-reviews/organs-on-chip-3d-cell-culture/3d-cell-culture-methods-and-applications-a-short-review/ [post_type] => reviews [post_type_name] => Review ) [1] => stdClass Object ( [preview] => Array ( 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identify the following two main purposes for the 3D cell market ) [utiliser_acf] => [most_popular] => [review_type] => 13 [insert] => ooc2 [citation_author_repeater] => [citation_publication_date] => [citation_keywords_repeater] => [description_GS] => [ID] => 734 [title] => 3D cell culture: market and industrial needs [permalink] => https://www.elveflow.com/microfluidic-reviews/organs-on-chip-3d-cell-culture/3d-cell-culture-market-industrial-needs/ [post_type] => reviews [post_type_name] => Review ) [2] => stdClass Object ( [preview] => Array ( [image] => Array ( [ID] => 58479 [id] => 58479 [title] => Microfluidic cell culture 1 [filename] => Microfluidic-cell-culture-1.jpg [filesize] => 95040 [url] => https://www.elveflow.com/wp-content/uploads/2021/08/Microfluidic-cell-culture-1.jpg [link] => https://www.elveflow.com/microfluidic-reviews/microfluidics-for-cell-biology/microfluidic-cell-culture-a-review/microfluidic-cell-culture-1/ [alt] => Microfluidic cell culture 1 [author] => 27 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[most_popular] => [review_type] => 11 [citation_author_repeater] => [citation_publication_date] => [citation_keywords_repeater] => [description_GS] => [ID] => 146463 [title] => Air-Liquid Interface and Optimized Cell Culture Substrates for Microfluidic Lung-on-a-Chip Applications [permalink] => https://www.elveflow.com/microfluidic-reviews/general-microfluidics/air-liquid-interface-lung-on-chip/ [post_type] => reviews [post_type_name] => Review ) [5] => stdClass Object ( [preview] => Array ( [image] => Array ( [ID] => 747 [id] => 747 [title] => Microfluidic for cell biology [filename] => Microfluidic-for-cell-biology.jpg [filesize] => 19325 [url] => https://www.elveflow.com/wp-content/uploads/2019/08/Microfluidic-for-cell-biology.jpg [link] => https://www.elveflow.com/microfluidic-reviews/microfluidics-for-cell-biology/concepts-and-methodologies/microfluidic-for-cell-biology/ [alt] => Microfluidic for cell biology [author] => 27 [description] => Microfluidic for cell biology [caption] 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[header__content] => Array ( [image__header] => [subtitle_text__header] => [title__header_ad] => [content__text__ad] => [link__header] => ) [header__content___sidebar_ads2] => Array ( [image__header_ads2] => [subtitle_text__header_ads2] => [title__header_ad_ads2] => [sub_title_2_ads2] => [content__text__ad_ads2] => [link__header_ads2] => ) [ID] => 746 [title] => Microfluidic for cell biology: Concepts and methodologies [permalink] => https://www.elveflow.com/microfluidic-reviews/microfluidics-for-cell-biology/concepts-and-methodologies/ [post_type] => reviews [post_type_name] => Review ) [6] => stdClass Object ( [preview] => Array ( [image] => Array ( [ID] => 755 [id] => 755 [title] => Cell culture under perfusion for microscopy live cell imaging [filename] => Cell-culture-under-perfusion-for-microscopy-live-cell-imaging.jpg [filesize] => 9898 [url] => https://www.elveflow.com/wp-content/uploads/2019/08/Cell-culture-under-perfusion-for-microscopy-live-cell-imaging.jpg [link] => https://www.elveflow.com/microfluidic-reviews/microfluidics-for-cell-biology/perfusion-for-live-cell-imaging-a-review/cell-culture-under-perfusion-for-microscopy-live-cell-imaging-4/ [alt] => Cell culture under perfusion for microscopy live cell imaging [author] => 27 [description] => Cell culture under perfusion for microscopy live cell imaging [caption] => Cell culture under perfusion for microscopy live cell imaging [name] => cell-culture-under-perfusion-for-microscopy-live-cell-imaging-4 [status] => inherit [uploaded_to] => 754 [date] => 2019-08-30 10:37:01 [modified] => 2024-08-19 14:21:18 [menu_order] => 0 [mime_type] => image/jpeg [type] => image [subtype] => jpeg [icon] => https://www.elveflow.com/wp-includes/images/media/default.png [width] => 335 [height] => 200 [sizes] => Array ( [thumbnail] => https://www.elveflow.com/wp-content/uploads/2019/08/Cell-culture-under-perfusion-for-microscopy-live-cell-imaging-150x150.jpg [thumbnail-width] => 150 [thumbnail-height] => 150 [medium] => https://www.elveflow.com/wp-content/uploads/2019/08/Cell-culture-under-perfusion-for-microscopy-live-cell-imaging-300x179.jpg [medium-width] => 300 [medium-height] => 179 [medium_large] => https://www.elveflow.com/wp-content/uploads/2019/08/Cell-culture-under-perfusion-for-microscopy-live-cell-imaging.jpg [medium_large-width] => 335 [medium_large-height] => 200 [large] => https://www.elveflow.com/wp-content/uploads/2019/08/Cell-culture-under-perfusion-for-microscopy-live-cell-imaging.jpg [large-width] => 335 [large-height] => 200 [1536x1536] => https://www.elveflow.com/wp-content/uploads/2019/08/Cell-culture-under-perfusion-for-microscopy-live-cell-imaging.jpg [1536x1536-width] => 335 [1536x1536-height] => 200 [2048x2048] => https://www.elveflow.com/wp-content/uploads/2019/08/Cell-culture-under-perfusion-for-microscopy-live-cell-imaging.jpg [2048x2048-width] => 335 [2048x2048-height] => 200 ) ) [texte] => Live-cell imaging is a non destructive method which focuses on the observation of live cells and is widely used in cellular biology research and biomedical industry. This short review will present some technical aspects and challenges encountered in this field. Nowadays, the most widely used cell culture system is the static culture, where the cells are cultivated inside Petri dishes or multiwall plates. 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This short review will present some technical aspects and challenges encounter in this field. ) [utiliser_acf] => [most_popular] => [review_type] => 14 [insert] => starter2 [citation_author_repeater] => Array ( [0] => Array ( [citation_author] => The Elveflow Team ) ) [citation_publication_date] => 2021/01/02 [citation_keywords_repeater] => Array ( [0] => Array ( [citation_keywords] => live cell imaging ) [1] => Array ( [citation_keywords] => microfluidics ) ) [description_GS] => Live-cell imaging is a non destructive method which focuses on the observation of live cells and is widely used in cellular biology research and biomedical industry. This short review will present some technical aspects and challenges encounter in this field. 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of organoids and organ-on-a-chip technologies in advancing drug testing, disease modeling, and personalized medicine ) [insert] => wpdrop [header__content___sidebar_ads2] => Array ( [image__header_ads2] => [subtitle_text__header_ads2] => [title__header_ad_ads2] => [sub_title_2_ads2] => [content__text__ad_ads2] => [link__header_ads2] => ) [utiliser_acf] => [most_popular] => [review_type] => 13 [citation_author_repeater] => [citation_publication_date] => [citation_keywords_repeater] => [description_GS] => [ID] => 152957 [title] => Organoids VS Organ-on-a-chip: A Comparative Review of Advanced In Vitro Models [permalink] => https://www.elveflow.com/microfluidic-reviews/organs-on-chip-3d-cell-culture/organoids-vs-organ-on-a-chip/ [post_type] => reviews [post_type_name] => Review ) [16] => stdClass Object ( [preview] => Array ( [image] => Array ( [ID] => 765 [id] => 765 [title] => free copyright microfluidic cell culture chamber 2 [filename] => 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[2048x2048-width] => 490 [2048x2048-height] => 294 ) ) [texte] => PDMS advantages and drawbacks for cell biology are here discussed. ) [utiliser_acf] => [most_popular] => [review_type] => 14 [insert] => starter2 [citation_author_repeater] => Array ( [0] => Array ( [citation_author] => The Elveflow Team ) ) [citation_publication_date] => 2021/01/06 [citation_keywords_repeater] => Array ( [0] => Array ( [citation_keywords] => pdms in biology ) [1] => Array ( [citation_keywords] => microfluidics ) ) [description_GS] => In biology, PDMS shows numerous advantages coming from its intrinsic properties. This review provides a critical analysis of its use. 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Indeed, there are several examples of gradient-dependent phenomena in nature. ) [utiliser_acf] => [most_popular] => [review_type] => 14 [insert] => ob1fc2 [citation_author_repeater] => Array ( [0] => Array ( [citation_author] => The Elveflow Team ) ) [citation_publication_date] => 2021/01/01 [citation_keywords_repeater] => Array ( [0] => Array ( [citation_keywords] => microfluidic gradients ) ) [description_GS] => Chemical gradients play a key role in many biological processes and regulate a number of cellular functions in vivo. Indeed, there are several examples of gradient-dependent phenomena in nature. [ID] => 741 [title] => Gradients generation for cell biology in microfluidics [permalink] => https://www.elveflow.com/microfluidic-reviews/microfluidics-for-cell-biology/gradients-generation-for-cell-biology-in-microfluidics/ [post_type] => reviews [post_type_name] => Review ) [19] => stdClass Object ( [header__content] => Array ( [image__header] => https://www.elveflow.com/wp-content/uploads/2024/03/Example-of-mechanosensing.png [subtitle_text__header] => Explore the pivotal role of shear stress in microfluidic systems and its impact on cellular mechanosensing. [title__header_ad] => Mechanosensing and Shear Stress in Microfluidics [content__text__ad] => Specifically, we will explore a mechanical force known as shear stress and its role in modulating cellular responses through a process known as mechanosensing. [link__header] => ) [preview] => Array ( [image] => Array ( [ID] => 135953 [id] => 135953 [title] => Example of mechanosensing [filename] => Example-of-mechanosensing.png [filesize] => 1001064 [url] => https://www.elveflow.com/wp-content/uploads/2024/03/Example-of-mechanosensing.png [link] => https://www.elveflow.com/microfluidic-reviews/general-microfluidics/microfluidic-shear-stress-mechanosensing-review/example-of-mechanosensing/ [alt] => Example of mechanosensing [author] => 37 [description] => [caption] => Figure 2. Example of mechanosensing, where integrins serve as the mechanosensor and tension is the mechanical stimuli. 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https://www.elveflow.com/wp-content/uploads/2024/05/Pharmacogenomics.webp [2048x2048-width] => 1024 [2048x2048-height] => 1024 ) ) [texte] => Pharmacogenomics is the study of how an individual’s genetic variants influence drug responses and treatment efficacy. ) [insert] => wpdrop [header__content___sidebar_ads2] => Array ( [image__header_ads2] => [subtitle_text__header_ads2] => [title__header_ad_ads2] => [sub_title_2_ads2] => [content__text__ad_ads2] => [link__header_ads2] => ) [utiliser_acf] => [most_popular] => [review_type] => 11 [citation_author_repeater] => [citation_publication_date] => [citation_keywords_repeater] => [description_GS] => [ID] => 139377 [title] => Microfluidics Devices in Pharmacogenomics Paving the way for precision medicine [permalink] => https://www.elveflow.com/microfluidic-reviews/general-microfluidics/pharmacogenomics-in-microfluidics-devices/ [post_type] => reviews [post_type_name] => Review ) [21] => stdClass Object ( [preview] => Array ( [image] => Array 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[texte] => Microfluidics is a term which appears more and more often in papers and scientific magazines; but, what exactly is microfluidics? ) [utiliser_acf] => [most_popular] => [review_type] => 11 [insert] => starter2 [citation_author_repeater] => [citation_publication_date] => [citation_keywords_repeater] => [description_GS] => [header__content] => Array ( [image__header] => [subtitle_text__header] => [title__header_ad] => [content__text__ad] => [link__header] => ) [header__content___sidebar_ads2] => Array ( [image__header_ads2] => [subtitle_text__header_ads2] => [title__header_ad_ads2] => [sub_title_2_ads2] => [content__text__ad_ads2] => [link__header_ads2] => ) [ID] => 639 [title] => Microfluidics applications: A short review [permalink] => https://www.elveflow.com/microfluidic-reviews/general-microfluidics/microfluidics-applications-a-short-review/ [post_type] => reviews [post_type_name] => Review ) [22] => stdClass Object ( [preview] => Array ( [image] => Array ( [ID] => 632 [id] => 632 [title] => Introduction to lab on a chip 2015 review history and future chip [filename] => Introduction-to-lab-on-a-chip-2015-review-history-and-future-chip.jpg [filesize] => 133468 [url] => https://www.elveflow.com/wp-content/uploads/2019/08/Introduction-to-lab-on-a-chip-2015-review-history-and-future-chip.jpg [link] => https://www.elveflow.com/microfluidic-reviews/general-microfluidics/introduction-to-lab-on-a-chip-review-history-and-future/introduction-to-lab-on-a-chip-2015-review-history-and-future-chip/ [alt] => Introduction to lab on a chip 2015 review history and future chip [author] => 27 [description] => Introduction to lab on a chip 2015 review history and future chip [caption] => Introduction to lab on a chip 2015 review history and future chip [name] => introduction-to-lab-on-a-chip-2015-review-history-and-future-chip [status] => inherit [uploaded_to] => 631 [date] => 2019-08-29 09:26:20 [modified] => 2024-08-19 14:21:17 [menu_order] => 0 [mime_type] => 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that integrates onto a single chip one or several analyses, which are usually done in a laboratory; analyses such as DNA sequencing or biochemical detection. [header__content] => Array ( [image__header] => [subtitle_text__header] => [title__header_ad] => [content__text__ad] => [link__header] => ) [header__content___sidebar_ads2] => Array ( [image__header_ads2] => [subtitle_text__header_ads2] => [title__header_ad_ads2] => [sub_title_2_ads2] => [content__text__ad_ads2] => [link__header_ads2] => ) [ID] => 631 [title] => Introduction to lab-on-a-chip 2024: review, history and future [permalink] => https://www.elveflow.com/microfluidic-reviews/general-microfluidics/introduction-to-lab-on-a-chip-review-history-and-future/ [post_type] => reviews [post_type_name] => Review ) [23] => stdClass Object ( [preview] => Array ( [image] => [texte] => Organ-on-chip companies developping innovative technologies ) [utiliser_acf] => [most_popular] => [review_type] => 13 [insert] => ooc2 [citation_author_repeater] => Array ( [0] => Array ( [citation_author] => Hadrien Mauriac ) ) [citation_publication_date] => 2020/12/02 [citation_keywords_repeater] => Array ( [0] => Array ( [citation_keywords] => organ on chip companies ) [1] => Array ( [citation_keywords] => organ on chip ) [2] => Array ( [citation_keywords] => microfluidics ) ) [description_GS] => Companies developing organ-on-chip devices focus on different areas and features. Here, we present an overview of the current market. 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Cell culture consists in growing cells in an artificial environment in order to study their behavior in response to their environment[1]. Different kinds of cell cultures can be found nowadays, and some would be more suited than others depending on its properties and applications.
In this report, we identify the following two main purposes for the 3D cell market
Microfluidic cell culture is an innovative technology to improve classical lab protocols
Ability to switch drugs in seconds or less in microfluidic chambers allows studying cell response at cells timescale.
Explore the intricacies of air-liquid interfaces and optimized cell culture substrates in microfluidic lung-on-a-chip systems.
Cell Biology: Microfluidic Concept and methologies for biologists to control the complete cellular microenvironment with microfluidics.
Live-cell imaging is a non destructive method which focuses on the observation of live cells and is widely used in cellular biology research and biomedical industry. This short review will present some technical aspects and challenges encountered in this field. Nowadays, the most widely used cell culture system is the static culture, where the cells are cultivated inside Petri dishes or multiwall plates. In static culture, the culture medium is supplied in a batch-wised manner.
Exploring Organ-on-a-Chip Technology: A comprehensive review
Microfluidic flow cells with embedded optical sensors, have been the most common choice for microenvironment monitoring like pH control.
This short note summarizes the methods and techniques employed to perform perfusion for live cell imaging and critical issues encountered.
Lab-On-a-Chip drug testing in Microfluidics
Explore the advanced microfluidic tumor-on-chip systems revolutionizing breast cancer research. How these systems offer precise drug testing.
Revolutionizing Microfluidics with Acoustic Particle Manipulation
Live-cell imaging is a non destructive method which focuses on the observation of live cells and is widely used in cellular biology research and biomedical industry. This short review will present some technical aspects and challenges encounter in this field.
The first live-cell imaging chambers were designed in the early twentieth century, shortly after mammalian cell culture techniques were developped
Discover the transformative potential of organoids and organ-on-a-chip technologies in advancing drug testing, disease modeling, and personalized medicine
PDMS advantages and drawbacks for cell biology are here discussed.
Multi-organs on chip could also allow us to witness the side effects of certain drugs on different organs, not limited to those that the treatment targets.
Chemical gradients play a key role in many biological processes and regulate a number of cellular functions in vivo. Indeed, there are several examples of gradient-dependent phenomena in nature.
Specifically, we will explore a mechanical force known as shear stress and its role in modulating cellular responses through a process known as mechanosensing.
Pharmacogenomics is the study of how an individual’s genetic variants influence drug responses and treatment efficacy.
Microfluidics is a term which appears more and more often in papers and scientific magazines; but, what exactly is microfluidics?
A lab-on-a-chip is a miniaturized device that integrates onto a single chip one or several analyses, which are usually done in a laboratory; analyses such as DNA sequencing or biochemical detection.
Organ-on-chip companies developping innovative technologies
Microfluidics involve different types of devices, and materials for microfluidic fabrication must be selected while bearing in mind all the requirements necessary for building a fine microfluidic device.
The liver is involved in more than 300 vital functions, but is mainly known for being part of the digestive tract, where it has the extremely important role of metabolizing both xenobiotics and nutrients (carbohydrates and lipids).
While many animal models have been used to study lung diseases, they lack sufficient similarity with human systems, leaving gaps in what is possible in animal-based platforms.
Although we take part in various research projects such as artificial photosynthesis, pathogen detection and stem cell differentiation, the ultimate goal of our entrepreneurial adventure is to accelerate anti-aging research.
Discover how gut-on-a-chip technology is revolutionizing intestinal research & drug development by replicating the gut's complex environment.
Microfluidics, i.e. the science and engineering of fluid flows in microscale, can be the answer for a more effective and targeted drug administration.
Since 2012, more and more people, companies or lab, have worked on the organ-on-a-chip. These cell cultures can, thanks to microfluidics, mimic the cells microenvironment of the human body. Thus, these chips could become wonderful search accelerators and we can hope that, in ten years, they could replace the animal testing. Finally, organs on chips could lead us to personalized medicine.
The integration of CRISPR-Cas9 with microfluidics has led to the development of innovative techniques for genetic editing and screening.
Today, MICROFLUIDICS is a distinct and major technological field, but 20 years ago it was not like this and its boundaries were not so well defined. The history of microfluidics is strictly related to several other areas
This review presents a few examples of applications where magnetic flux sources, magnetic particles and microfluidics are combined in order to perform particle sorting and handling.
This review focuses on sodium alginate and its applications in microfluidics
Finding the right technique for particle encapsulation using micro and nanoparticles is key for a successful particle encapsulation protocol.
It could be extremely interesting to build a human-on-chip that will model the interactions between different organs, but it is also essential to develop simulations of tissue-tissue interfaces and more generally of local organ behavior.
Introduction to thermoelectric sensor | Microfluidics immunosensors offer multiple advantages over the conventional immunoassays that include improved reaction rate, reduced time for incubation of the reactants, and decreased reagents and sample consumption. Moreover, miniaturization and integration of the multiple assay components permit automation, precise flow control, increased reproducibility, and the possibility for high-throughput analysis.
A heart-on-chip is a microfluidic chip reproducing the mechanisms of a heart, in order to test medicine quickly and observe the reaction of heart cells. Great care is given to mimic the mechanics of a heart in an artificial structure, lined with live heart cells.
High accuracy microfluidic pumps are important for many applications from dynamic cell culture to droplet or nanoparticle generation.
This review presents an overview of the different techniques developed over the last decade to regulate the temperature within microfluidic systems.
Coronavirus (SARS-CoV2) diagnostic through microfluidics
Microfabrication techniques for a circular channel
The Dynamics of Fungal Spore Dispersal: Insights from Microfluidic Models
At the beginning of the third millennium, due to prolonged aging, neurodevelopmental disorders are growing and a much deeper understanding of the brain is necessary.
Microfluidics is both the science which studies the behaviour of fluids through micro-channels and the technology of manufacturing microminiaturized devices containing chambers and tunnels through which fluids flow or are confined.
This review introduces the field of microfluidics and provides an overview of the advantages, disadvantages, and current applications of microfluidics in chemistry.
Polydimethylsiloxane, called PDMS or dimethicone, is a polymer widely used for the fabrication and prototyping of microfluidic chips.
One of the key criteria to choose your microfluidic device material is its chemical resistance. This review will help you choose one depending on your application.
Polydimethylsiloxane, called PDMS or dimethicone, is a polymer widely used for the fabrication and prototyping of microfluidic chips. It is a mineral-organic polymer (a structure containing carbon and silicon)
Microfluidics: definitions. This short review aims to cover the main microfluidics definitions from the simple word by word science to the advantages & applications
Air bubbles are among the most recurring issues in microfluidics. Because of the micrometric dimensions of the tubes and channels
This review focuses on point of care (POC) diagnostic devices for pathogen detection
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stain cells for dynamic cell culture with different microfluidic setups. ) [insert] => ooc2 [utiliser_acf] => [most_popular] => [application_type] => 8 [citation_author_repeater] => [citation_publication_date] => [citation_keywords_repeater] => [description_GS] => [pdf_application_note_link] => [ID] => 19712 [title] => How to stain cells cultured in a microfluidic chip? 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The micrometer scale of microfluidic devices is particularly adapted to work with cells. ) [utiliser_acf] => [most_popular] => [application_type] => 8 [insert] => starter2 [citation_author_repeater] => Array ( [0] => Array ( [citation_author] => The Elveflow Team ) ) [citation_publication_date] => 2021/01/05 [citation_keywords_repeater] => Array ( [0] => Array ( [citation_keywords] => cell biology ) ) [description_GS] => Microfluidics is widely used to develop tools for cell biology. The micrometer scale of microfluidic devices is particularly adapted to work with cells. 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[description_GS] => This application note explains how pressure-driven flow control works, the advantages & disadvantages of the different technologies, and the technical choice to make to how to perform effectively your microfluidic experiment. 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[header__content] => Array ( [image__header] => [subtitle_text__header] => [title__header_ad] => [content__text__ad] => [link__header] => ) [pdf_url_for_download_in_contact_form] => [header__content___sidebar_ads2] => Array ( [image__header_ads2] => [subtitle_text__header_ads2] => [title__header_ad_ads2] => [sub_title_2_ads2] => [content__text__ad_ads2] => [link__header_ads2] => ) [ID] => 103567 [title] => Biofilm testing under flow [permalink] => https://www.elveflow.com/microfluidic-applications/microfluidic-cell-culture/biofilm-testing-under-flow/ [post_type] => note [post_type_name] => Application note ) [13] => stdClass Object ( [preview] => Array ( [image] => Array ( [ID] => 495 [id] => 495 [title] => recirculating perfusion microfluidic system [filename] => recirculating-perfusion-microfluidic-system.jpg [filesize] => 52068 [url] => https://www.elveflow.com/wp-content/uploads/2019/08/recirculating-perfusion-microfluidic-system.jpg [link] => https://www.elveflow.com/microfluidic-applications/setup-microfluidic-flow-control/recirculating-perfusion-microfluidic-system/recirculating-perfusion-microfluidic-system-5/ [alt] => recirculating perfusion microfluidic system [author] => 27 [description] => recirculating perfusion microfluidic system [caption] => recirculating perfusion microfluidic system [name] => recirculating-perfusion-microfluidic-system-5 [status] => inherit [uploaded_to] => 494 [date] => 2019-08-27 10:39:03 [modified] => 2024-08-19 14:21:17 [menu_order] => 0 [mime_type] => image/jpeg [type] => image [subtype] => jpeg [icon] => https://www.elveflow.com/wp-includes/images/media/default.png [width] => 800 [height] => 533 [sizes] => Array ( [thumbnail] => https://www.elveflow.com/wp-content/uploads/2019/08/recirculating-perfusion-microfluidic-system-150x150.jpg [thumbnail-width] => 150 [thumbnail-height] => 150 [medium] => https://www.elveflow.com/wp-content/uploads/2019/08/recirculating-perfusion-microfluidic-system-300x200.jpg [medium-width] => 300 [medium-height] => 200 [medium_large] => https://www.elveflow.com/wp-content/uploads/2019/08/recirculating-perfusion-microfluidic-system-768x512.jpg [medium_large-width] => 768 [medium_large-height] => 512 [large] => https://www.elveflow.com/wp-content/uploads/2019/08/recirculating-perfusion-microfluidic-system.jpg [large-width] => 800 [large-height] => 533 [1536x1536] => https://www.elveflow.com/wp-content/uploads/2019/08/recirculating-perfusion-microfluidic-system.jpg [1536x1536-width] => 800 [1536x1536-height] => 533 [2048x2048] => https://www.elveflow.com/wp-content/uploads/2019/08/recirculating-perfusion-microfluidic-system.jpg [2048x2048-width] => 800 [2048x2048-height] => 533 ) ) [texte] => The MUX Inj is a bidirectional 6-port / 2 position valve allowing to perform switches between two setup configurations. One application is to make a fluid recirculation set-up with a flow rate always going through the chip in the same direction. In this application note, we walk you through the steps of setting up a unidirectional recirculation through a semi permeable membrane. ) [utiliser_acf] => [most_popular] => [application_type] => 6 [insert] => ooc2 [citation_author_repeater] => Array ( [0] => Array ( [citation_author] => Guilhem Velvé Casquillas ) ) [citation_publication_date] => 2021/01/04 [citation_keywords_repeater] => Array ( [0] => Array ( [citation_keywords] => fluid recirculation ) ) [description_GS] => The MUX Inj is a bidirectional 6-port / 2 position valve allowing to perform switches between two setup configurations. One application is to make a recirculating set-up with a flow rate always going through the chip in the same direction. In this application note, we walk you through the steps of setting up a unidirectional recirculation through a semi permeable membrane. 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The easiest solution is to replace the liquid injected, but it is often not possible: ) [utiliser_acf] => [most_popular] => [application_type] => 6 [insert] => ob1fc2 [citation_author_repeater] => Array ( [0] => Array ( [citation_author] => Guilhem Velvé Casquillas ) ) [citation_publication_date] => 2021/01/04 [citation_keywords_repeater] => Array ( [0] => Array ( [citation_keywords] => fast sample switch ) [1] => Array ( [citation_keywords] => microfluidics ) ) [description_GS] => Changing the injected liquid inside a microfluidic device has numerous applications, such as sequential sample injection for biochemistry and flow chemistry, or medium switch for cell biology and 3D cell culture on chip. The easiest solution is to replace the liquid injected, but it is often not possible: [pdf_application_note_link] => [ID] => 519 [title] => Controlled medium switch on a microfluidic chip [permalink] => https://www.elveflow.com/microfluidic-applications/setup-microfluidic-flow-control/controlled-medium-switch-on-a-microfluidic-chip/ [post_type] => note [post_type_name] => Application note ) [15] => stdClass Object ( [preview] => Array ( [image] => Array ( [ID] => 18326 [id] => 18326 [title] => Setup bronkhorst elveflow flow controller elveflow microfluidics [filename] => Setup-bronkhorst-elveflow-flow-controller-elveflow-microfluidics.jpg [filesize] => 103324 [url] => https://www.elveflow.com/wp-content/uploads/2019/08/Setup-bronkhorst-elveflow-flow-controller-elveflow-microfluidics.jpg [link] => https://www.elveflow.com/microfluidic-products/microfluidics-flow-measurement-sensors/microfluidic-flow-sensor-coriolis/setup-bronkhorst-elveflow-flow-controller-elveflow-microfluidics/ [alt] => Setup bronkhorst elveflow flow controller elveflow microfluidics [author] => 27 [description] => Setup bronkhorst elveflow flow controller elveflow microfluidics [caption] => Setup bronkhorst elveflow flow controller elveflow microfluidics [name] => setup-bronkhorst-elveflow-flow-controller-elveflow-microfluidics [status] => inherit [uploaded_to] => 382 [date] => 2020-07-01 13:02:39 [modified] => 2023-12-15 16:32:44 [menu_order] => 0 [mime_type] => image/jpeg [type] => image [subtype] => jpeg [icon] => https://www.elveflow.com/wp-includes/images/media/default.png [width] => 1000 [height] => 618 [sizes] => Array ( [thumbnail] => https://www.elveflow.com/wp-content/uploads/2019/08/Setup-bronkhorst-elveflow-flow-controller-elveflow-microfluidics-150x150.jpg [thumbnail-width] => 150 [thumbnail-height] => 150 [medium] => https://www.elveflow.com/wp-content/uploads/2019/08/Setup-bronkhorst-elveflow-flow-controller-elveflow-microfluidics-300x185.jpg [medium-width] => 300 [medium-height] => 185 [medium_large] => https://www.elveflow.com/wp-content/uploads/2019/08/Setup-bronkhorst-elveflow-flow-controller-elveflow-microfluidics-768x475.jpg [medium_large-width] => 768 [medium_large-height] => 475 [large] => https://www.elveflow.com/wp-content/uploads/2019/08/Setup-bronkhorst-elveflow-flow-controller-elveflow-microfluidics.jpg [large-width] => 1000 [large-height] => 618 [1536x1536] => https://www.elveflow.com/wp-content/uploads/2019/08/Setup-bronkhorst-elveflow-flow-controller-elveflow-microfluidics.jpg [1536x1536-width] => 1000 [1536x1536-height] => 618 [2048x2048] => https://www.elveflow.com/wp-content/uploads/2019/08/Setup-bronkhorst-elveflow-flow-controller-elveflow-microfluidics.jpg [2048x2048-width] => 1000 [2048x2048-height] => 618 ) ) [texte] => Flow regulation is a compulsory operation in most of the microfluidics operations. In some applications such as 2D or 3D cell culture, flow regulation is essential since accurate micro-environmental parameters control is required. Elveflow do it’s best to make this operation as easy as possible to help you to focus on what really matter in your setup. ) [utiliser_acf] => [most_popular] => [application_type] => 6 [insert] => ob1fc2 [citation_author_repeater] => Array ( [0] => Array ( [citation_author] => The Elveflow team ) ) [citation_publication_date] => 2021/01/06 [citation_keywords_repeater] => Array ( [0] => Array ( [citation_keywords] => flow regulation parameters ) ) [description_GS] => Flow regulation is a compulsory operation in most of the microfluidics operations. In some applications such as 2D or 3D cell culture, flow regulation is essential since accurate micro-environmental parameters control is required. Elveflow do it’s best to make this operation as easy as possible to help you to focus on what really matter in your setup. 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Nekimken et al. published in Lab Chip in January 2017. The research team introduced a novel microfluidic device that enables high-resolution imaging of cellular deformations in response to precise mechanical stimuli to the surface of the C. Elegans worm cuticle. ) [insert] => ob1fc2 [utiliser_acf] => [most_popular] => [application_type] => 8 [citation_author_repeater] => Array ( [0] => Array ( [citation_author] => The elveflow team ) ) [citation_publication_date] => 2017/12/01 [citation_keywords_repeater] => Array ( [0] => Array ( [citation_keywords] => c. elegans ) [1] => Array ( [citation_keywords] => microfluidics ) [2] => Array ( [citation_keywords] => deformation ) ) [description_GS] => Do you know how to perform high-resolution imaging of cellular deformations in response to precise mechanical stimuli to the surface of the C. Elegans worm cuticle? 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This tool has been designed to help researchers and especially non specialists of the microfluidics field. It helps you assess key parameters to configure your microfluidic experiment. ) [insert] => starter2 [utiliser_acf] => [most_popular] => [application_type] => 6 [citation_author_repeater] => Array ( [0] => Array ( [citation_author] => The Elveflow team ) ) [citation_publication_date] => 2020/12/15 [citation_keywords_repeater] => Array ( [0] => Array ( [citation_keywords] => microfluidic calculator ) [1] => Array ( [citation_keywords] => shear stress ) [2] => Array ( [citation_keywords] => ) ) [description_GS] => Elveflow developped a free online microfluidic calculator. This tool has been designed to help researchers and especially non specialists of the microfluidics field. It helps you assess key parameters to configure your microfluidic experiment. 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This application note aims to help you maintain a controlled flow rate during a flow line switch thanks to the Mux Distributor. This valve allows to switch between up to 10 lines to inject several fluids sequentially in your system. It has many applications such as sequential sample injection for biochemistry and flow chemistry, or medium switch for cell biology on chip. ) [utiliser_acf] => [most_popular] => [application_type] => 6 [insert] => secinjection2 [citation_author_repeater] => Array ( [0] => Array ( [citation_author] => The Elveflow team ) ) [citation_publication_date] => 2021/01/06 [citation_keywords_repeater] => Array ( [0] => Array ( [citation_keywords] => microfluidic valve switching ) ) [description_GS] => Maintain a controlled flow rate? This application note aims to help you maintain a controlled flow rate during a flow line switch thanks to the Mux Distributor. This valve allows to switch between up to 10 lines to inject several fluids sequentially in your system. It has many applications such as sequential sample injection for biochemistry and flow chemistry, or medium switch for cell biology on chip. 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One application is to perform a stable and unidirectional fluid recirculation or to inject a precisely controlled volume of drug. This application note focuses on the example and will walk you through the steps of a successful experiment. ) [utiliser_acf] => [most_popular] => [application_type] => 6 [insert] => wpdrop [citation_author_repeater] => Array ( [0] => Array ( [citation_author] => The Elveflow team ) [1] => Array ( [citation_author] => ) ) [citation_publication_date] => 2021/01/06 [citation_keywords_repeater] => Array ( [0] => Array ( [citation_keywords] => microfluidic injection ) ) [description_GS] => The MUX inj is a bidirectional 6-port / 2 position valve allowing to perform switches between two set-up configurations. One application is to perform a stable and unidirectional fluid recirculation or to inject a precisely controlled volume of drug. This application note focuses on the example and will walk you through the steps of a successful experiment. 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environment (drug screening or cell culture) with an Elveflow® MUX as shown on these pictures: ) [utiliser_acf] => [most_popular] => [application_type] => 6 [insert] => secinjection2 [pdf_application_note_link] => [citation_author_repeater] => [citation_publication_date] => [citation_keywords_repeater] => [description_GS] => [ID] => 510 [title] => Microfluidic controlled drug switch [permalink] => https://www.elveflow.com/microfluidic-applications/setup-microfluidic-flow-control/microfluidic-controlled-drug-switch/ [post_type] => note [post_type_name] => Application note ) [27] => stdClass Object ( [preview] => Array ( [image] => Array ( [ID] => 538 [id] => 538 [title] => microfluidic droplet on demand [filename] => microfluidic-droplet-on-demand.png [filesize] => 7044 [url] => https://www.elveflow.com/wp-content/uploads/2019/08/microfluidic-droplet-on-demand.png [link] => 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A simple guide to do dynamic cell culture by automating cell seeding in a microlfuidic chip
In this application note we describe how to do cell perfusion for dynamic cell culture and a way to enable uni-directional recirculation of medium.
This application note proposes a microfluidic cardiac cell culture model (μCCCM) to recreate mechanical loading conditions observed in the native heart (in both normal and pathological conditions) by using an Elveflow OB1 pressure and flow controller.
In this application note we describe how to create a medium recirculation for dynamic cell culture with a microfluidic setup.
This application note describes how co-culture of different cell types in separate but interconnected chambers is possible in a microfluidic platform
In this application note we describe how to stain cells for dynamic cell culture with different microfluidic setups.
Microfluidics is widely used to develop tools for cell biology. The micrometer scale of microfluidic devices is particularly adapted to work with cells.
This application note presents how to perform cell culture on chip using the Cell and Biology Pack and the MicroSlides developed by ALine Inc.
In this application note we describe how to set up medium recirculation by using microfluidic valves
Medium switch is widely used in cell biology. One application is the study of cell behavior under given flow conditions for different samples. In this tutorial, we walk you through the steps of a fast and stable medium switch using IBIDI© flow cells.
Study the impact of molecular transport on cell cultures with a cross flow membrane chip and microfluidic instruments.
This application note explains how pressure-driven flow control works, the advantages & disadvantages of the different technologies, and the technical choice to make to how to perform effectively your microfluidic experiment.
Biofilm testing using a simple microfluidic chip channel for in situ observation of their development under flow conditions.
The MUX Inj is a bidirectional 6-port / 2 position valve allowing to perform switches between two setup configurations. One application is to make a fluid recirculation set-up with a flow rate always going through the chip in the same direction. In this application note, we walk you through the steps of setting up a unidirectional recirculation through a semi permeable membrane.
Changing the injected liquid inside a microfluidic device has numerous applications, such as sequential sample injection for biochemistry and flow chemistry, or medium switch for cell biology and 3D cell culture on chip. The easiest solution is to replace the liquid injected, but it is often not possible:
Flow regulation is a compulsory operation in most of the microfluidics operations. In some applications such as 2D or 3D cell culture, flow regulation is essential since accurate micro-environmental parameters control is required. Elveflow do it’s best to make this operation as easy as possible to help you to focus on what really matter in your setup.
This article covers the work of Adam L. Nekimken et al. published in Lab Chip in January 2017. The research team introduced a novel microfluidic device that enables high-resolution imaging of cellular deformations in response to precise mechanical stimuli to the surface of the C. Elegans worm cuticle.
Elveflow developped a free online microfluidic calculator. This tool has been designed to help researchers and especially non specialists of the microfluidics field. It helps you assess key parameters to configure your microfluidic experiment.
Maintain a controlled flow rate? This application note aims to help you maintain a controlled flow rate during a flow line switch thanks to the Mux Distributor. This valve allows to switch between up to 10 lines to inject several fluids sequentially in your system. It has many applications such as sequential sample injection for biochemistry and flow chemistry, or medium switch for cell biology on chip.
This article covers the development of an experimental method enabling Shahar Sukenik et al. to study protein interactions and detect the dissociation of GAPDH and PGK proteins in order to quantify their stoichiometry directly inside the cell by modulating the cell-volume
The MUX inj is a bidirectional 6-port / 2 position valve allowing to perform switches between two set-up configurations. One application is to perform a stable and unidirectional fluid recirculation or to inject a precisely controlled volume of drug. This application note focuses on the example and will walk you through the steps of a successful experiment.
This application note explains how to study bacteria adaptation to stress and environmental changes such as antibiotics.
Prostate cancer is the second leading cause of cancer-related death for men. Circulating tumor cells (CTCs) are considered as a marker of early cancer diagnosis and disease severity. Their screening in blood is thus crucial to detect metastatic stage in cancer patients.
Microfluidic sequential production and trapping of droplets for in situ optical analysis.
This application note describes how to combine and synchronise liquid perfusion and imaging using an Olympus spinning disc confocal microscope together with an Elveflow pressure-driven flow controlled microfluidic system.
This application note explores the basic principle of pneumatic pumps and a flow controller based on the basic principle of pneumatic pumps, known as pressure driven flow control. It also demonstrates the applications of pressure driven flow control in a range of industrial & research fields.
This application note shows how to easily perform controlled drug switches on an hypothetic chemical or biological environment (drug screening or cell culture) with an Elveflow® MUX as shown on these pictures:
Active droplet generation in microfluidics is of high interest for a wide range of applications. It provides an additional degree of freedom in manipulating both the size and the formation frequency of micro-droplets. This additional control is extremely desirable for complex operations which rely on the accurate control of both parameters.
This application note describes how to generate a controlled flow inside a microfluidic chip and stop it completely (zero flow) by using an Elveflow® pressure control instrument (OB1) and valves.
Because of fluidic compliance of tubing and chip, achieving stop flow into a microfluidic device remain a challenge with conventional setup. One solution to achieve stop flow in hundreds of milliseconds into a microchip without residual flow is to use a pressure controller coupled with flow switch.
The application note describes how to convert various units of shear stress and/or pressure from one to another: shear stress conversion from Pascal, atmosphere, and N/m²...!
Elveflow's microfluidic calculator permits to calculate flow rate in microfluidics dependent upon the microfluidic system from the device, the tubing to your fluid properties.
In this review we will explain the operating principle of pressure driven flow control, the advantages / disadvantages of the different technologies and how to choose between a peristaltic pump and pressure control depending on your requirements.
Elveflow's developped a microfluidic resistance calculator dependent upon the microfluidic system from the flow rate, the device, the tubing to your fluid properties.
The application note describes how to convert various units of viscosity from one to another: viscosity conversion from Poise, Pa.s, Dyn.s/cm²...
Until recently, microfluidic devices have been employed to support tissue-engineering experiments on basal lamina, vascular tissue, liver, bone, cartilage and neurons as well as organ-on-chips.
Microfluidics for microscopy imaging in plant biology allows to observe, in vivo, the biological response of plant roots to various stimuli.
This application note will show you how highly monodispersed droplets can be easily generated to encapsulate single spore of fungus using a microfluidic droplet generation system.
In this application note, we will describe how to perform an automated and fast medium switch thanks to the Perfusion Pack.
Highly monodispersed alginate beads can be easily generated with a microfluidic droplet generation system.
Droplet-based microfluidics and single spore encapsulation offer the key for a breakthrough in antifungal screening and fungicide discovery.
Fluorescence reader for microfluidic qPCR: faster, more sensitive and less expensive than most optical microscopes, it is a smart alternative for real-time fluorescence measurements of your on-chip qPCR signal.
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The microfluidic calculator will help you determine flow rate, pressure to apply, the best tubing resistance length for your setup, wall shear stress for biology applications, cell culture, and many more. 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Within a fast, easy and inexpensive microstructuration method, microfluidic devices are fabricated in less 1 min. Furthermore microfabrication can be performed using a hot-embossing machine or a very simple press equipment, downstream FlexdymTM is amenable for rapid manufacturing technology such as injection molding, roll-to-roll, etc. Either on glass, various polymer and for monolithic devices, your microfluidic devices can be assembled and bonded easily without surface treatments and pressure loads, thanks to the FlexdymTM mechanical properties (i. e. Young Modulus 1.15 MPa). 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The authors describe the use of a dynamic dual micro-valve system, which can be used to screen cells based on their size and print single-cells into separate cell culture chambers.
Discover how a microfluidic constriction assay in large cardiac spheroids replicates myocardial ischemia and fibrotic remodeling to advance cardiovascular research.
Design, fabrication and application of microfluidics-based flexible microdevices to apply mechanical cell compression and stimulation
The study explores a method to dynamically screen and print single cells using microfluidics with pneumatic microvalves.
Discover how mechanical cues influence pathogen-host interactions, and the challenges in capturing these in real-time microscopy.
The authors developed, tested and validated a method that applies a pressure-driven flow controller to fabricate biocompatible micron-scale silk fibers by microfluidic wet spinning.
This review article explores the functioning of a human bladder on chip model to study the key features of bladder physiology that are important to early UPEC infection.
Electrokinetic sandwich assay and DNA mediated charge amplification
This short review presents the fabrication and testing of a 3D microfluidic micromixing device to perform on-chip particle manipulation.
Thus far, 3D laser-micro and nanoprinting offered a widespread alternative to prepare complex but single constituent 3D microfluidic structure. This work proposes a system based on a microfluidic chamber integrated into a state-of-the-art laser lithography apparatus that allows the use of several materials to 3D print complex structures in the most effective manner.
This article investigates the behaviour of immiscible two-phase flow through porous media focusing on how pore-scale processes impact macroscopic fluid front behaviour.
The authors describe the use of microfluidics for the fabrication of nanohydrogels made from hyaluronic acid derivatives, for drug-delivery.
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In combination with the flow sensor (MFS or BFS series), the OB1 flow controller allows to have a very stable medium perfusion. Moreover, the medium change is easily done using the MUX Distribution that allows to switch between 12 solutions. All these operations can be performed using only the ESI software interface. The software allows you to fine tune the flow parameters as well as to automate your experiment using the intuitive scheduler. Advantages Control both pressure & flow rate: Ideal for shear stress assays Perform fast switches between media or drugs: For imaging cell response to various media or drugs Stable & pulseless flow rate: No more coverslip expansion and cell stress Large range of flow rates: From 10nL/min to 5 mL/min Design flow injection sequences: Create complex patterns, such as oscillating flow to mimic physiological conditions Recirculation loop: Ideal for long assays Instantaneous stop flow: For controlled solution exposure experiments, such as calcium imaging Our dedicated pack can be adapted for more complex and advanced Cell & Biology experiments such as using 20 solutions, choosing the right microfluidic chip, removing bubbles or to have multiple chip/inlets perfusion. Contact us for more information! Computer: Control all the parameters with our software, and automate your experiment by creating injection sequences. Pressure & flow controller: Impose a given pressure in order to create a stable and pulseless flow rate. Splitter: Multiple your reservoir’s pressure inlet from a single controller pressure output using our splitter Reservoirs: Contain your medium or samples. Various sizes are available, from Eppendorf to bottles. Rotative valve | Mux Distribution: Select the injected liquid. Flow Sensor: Monitor and control the flow rate in real time. Perfusion chamber or microfluidic chip: Elveflow can help choosing a microfluidic chip fitting your cell culture requirements. Microfluidic calculator to estimate the shear stress in your cell culture chamber To help you determine your flow rate, pressure to apply, the best tubing resistance length for your setup, wall shear stress for biology applications, cell culture, and many more… Elveflow provides its microfluidic calculator and…to make the most of our microfluidic calculator, find below a set of dedicated application notes: What is a microfluidic calculator? Viscosity conversion Shear stress calculator How to calculate the flow rate with microfluidics Microfluidic resistance calculator [image] => ) [applications] => Array ( [texte] => Learn more on dynamic cell culture using this pack. Learn how to automate cell seeding with this pack. Learn more on long-term medium perfusion using this pack BIOLOGY-RELATED FIELDS OF APPLICATION: Cell confinement assays Dynamic single cell screening Drug screening Cell response to medium change Live cell imaging C.elegans stimulation Cell culture on chip Toxicity tests 3D cell culture Bioreactor studies Stem cells assays Cells entrapment APPLICATION NOTES Cell culture for live cell imaging How to stain cells cultured in a microfluidic chip for dynamic cell culture? medium switch and custom flow patterns in IBIDI© chips Microfluidic cardiac cell culture model Stimulation of C.Elegans mechanoreceptor neurons using microfluidics Circulating Tumor Cells entrapment using microfluidics Tissue-engineering: Heart Ventricule assisted by a microfluidic pressure pump Weak protein interactions quantified using a microfluidic pump …and more cell culture application notes Reviews Microfluidic gradient generators for cell biology Perfusion for live cell imaging: Methods and techniques Cell culture with perfusion systems For more, Check out our list of Cell biology-related reviews. Publications A. Chen et al., “Single Cell Mass Spectrometry with a Robotic Micromanipulation System for Cell Metabolite Analysis,” in IEEE Transactions on Biomedical Engineering, doi: 10.1109/TBME.2021.3093097. Proton gradients from light-harvesting E. coli control DNA assemblies for synthetic cells, Kevin Jahnke et al. Nature Communications (2021). DOI: 10.1038/s41467-021-24103-x A microfluidic device enabling drug resistance analysis of leukemia cells via coupled dielectrophoretic detection and impedimetric counting, Yağmur Demircan Yalçın et al., Scientific Reports (2021). DOI: 10.1038/s41598-021-92647-5 Highly sensitive wide-range target fluorescence biosensors of high-emittance metasurfaces, Masanobu Iwanaga, Biosensors and Biolectronics (2021). DOI: 10.1016/j.bios.2021.113423 Critical Frequency and Critical Stretching Rate for Reorientation of Cells on a Cyclically Stretched Polymer in a Microfluidic Chip, Jiandong Ding et al., ACS Applied Materials & Interfaces (2021). DOI: 10.1021/acsami.0c21186 In vitro skin model for characterization of sunscreen substantivity upon perspiration, Fatemeh Keshavarzi et al., International Journal of Cosmetic Science (2021). DOI: 10.1111/ics.12703 Electrokinetic sandwich assay and DNA mediated charge amplification for enhanced sensitivity and specificity, Siddharth Sourabh Sahu et al. Biosensors & Bioelectronics (2021). DOI: 10.1016/j.bios.2020.112917 The method to dynamically screen and print single cells using microfluidics with pneumatic microvalves, Chang Chen et al., MethodX (2020). DOI: 10.1016/j.mex.2020.101190 Cyclic on-chip bacteria separation and preconcentration, Vitaly Ryzhkov et al., Scientific Reports (2020). DOI: 1038/s41598-020-78298-y Synthetic Biology Bicistronic Designs Support Gene Expression Equally Well in vitro and in vivo, Owen Koucky et al., American Journal of Undergraduate Research (2020). DOI: 10.33697/ajur.2020.012 Zhu Z., Geng Y., Wang Y. (2021) Monitoring Single S. cerevisiae Cells with Multifrequency Electrical Impedance Spectroscopy in an Electrode-Integrated Microfluidic Device. In: Marchisio M.A. (eds) Computational Methods in Synthetic Biology. Methods in Molecular Biology, vol 2189. Humana, New York, NY. DOI: 10.1007/978-1-0716-0822-7_9 Investigating the Interaction Between Circulating Tumor Cells and Local Hydrodynamics via Experiment and Simulations Pepona, M., Balogh, P., Puleri, D.F. et al. . Cel. Mol. Bioeng. (2020). DOI: 10.1007/s12195-020-00656-7 A drug-compatible and temperature-controlled microfluidic device for live-cell imaging, Open Biology; Jul, 2016; T. Chen et al; DOI : 10.1098/rsob.160156 A microfluidic gradient generator to simulate the oxygen microenvironment in cancer cell culture; Microelectronic Engineering; Aug, 2018; Louise Orcheston-Findlay et el; DOI : 10.1016/j.mee.2018.04.011 Geometric Friction Directs Cell Migration; Physical Review Letters; Jul, 2013; Le Berre et al; DOI : 10.1103/PhysRevLett.111.198101 An Integrated Microfluidic Chip and Its Clinical Application for Circulating Tumor Cell Isolation and Single‐Cell Analysis; Cytometry; Oct, 2019; Mingxin Xu et al; DOI : 10.1002/cyto.a.23902 Mitotic Rounding Alters Cell Geometry to Ensure Efficient Bipolar Spindle Formation; Developmental Cell; Apr, 2013; Oscar M. Lancaster et al; DOI : 10.1016/j.devcel.2013.03.014 ) [specifications] => Array ( [texte] => The amazing benefits of microfluidics can be applied to many Cell & Biology perfusion experiments and applications. Therefore, the content of the Cell & Biology Pack can be adjusted to suit your specific needs. Contact us for more details. CONTENT Pressure & flow controller (OB1) Rotative valve (MUX Distributor) Microfluidic Flow Sensor (MFS) Reservoirs Pressure splitter manifold Tubing and connectors Software and SDK libraries (C++, Python, MATLAB, LabVIEW) OPTIONS Additional pumping channels Additional flow rate sensors Microfluidics chips Computer Microscope and camera [image] => ) [download] => Array ( [fichiers] => ) [faq] => Array ( [items] => ) [more] => Array ( [more_title] => Videos [texte] => Cell & biology webinars Cell & biology videos [image] => [video] => ) [outline1] => Array ( [text] => [image] => ) [customization] => Array ( [title] => [] => [image] => [texte_1] => [texte_2] => [texte_3] => ) [outline2] => Array ( [image] => [text] => ) [produits_associes] => Array ( [0] => 97261 [1] => 350 [2] => 375 [3] => 382 ) [application_packs_on] => [software_on] => 1 [distributors_on] => [services_on] => [citation_author_repeater] => [citation_publication_date] => [citation_keywords_repeater] => [description_GS] => [testimonials_prod] => Array ( [0] => Array ( [testimonial_] => Array ( [testimonial_text] => I appreciate rapid setup time and the fact that we can very precisely adjust the flow rates through the user-friendly interface. The dynamic and responsive engineering team was an additional benefit. [testimonial_author] => Dr. Caglar Elbuken, UNAM Bilkent University, Turkey [testimonial_product] => OB1 pressure-driven flow controller user ) ) [1] => Array ( [testimonial_] => Array ( [testimonial_text] => We really appreciate the stability and low response time of the flow controller, which is important when various flow conditions must be applied successively. The flow can be precisely and easily controlled thanks to a user-friendly interface. [testimonial_author] => Dr. Marie Frénéa-Robin, Laboratoire AMPERE Université Claude Bernard LYON 1, France [testimonial_product] => OB1 pressure-driven flow controller user ) ) [2] => Array ( [testimonial_] => Array ( [testimonial_text] => Elveflow provided an excellent solution to our problem, which was how to tightly control minute flows in multiple microfluidic channels. Elveflow’s support has been excellent and software development is continuing. [testimonial_author] => Dr. Robert Ward, Massey University, United States [testimonial_product] => OB1 pressure-driven flow controller user ) ) [3] => Array ( [testimonial_] => Array ( [testimonial_text] => Overall we are satisfied with the equipment and highly recommend it to anyone who is working in the microfluidics field. [testimonial_author] => Dr. Weiqiang Chen, Biomechanics Laboratory (IBBL) University of Michigan, United States [testimonial_product] => OB1 pressure-driven flow controller ) ) [4] => Array ( [testimonial_] => Array ( [testimonial_text] => I was suprised how quickly I was able to get things to work once I got going. [testimonial_author] => Jamie Stover, Research Assistant, M. Zernicka-Goetz Lab at California Institute of Technology (Caltech) [testimonial_product] => OB1 pressure-driven flow controller user ) ) ) [header__content___sidebar_ads2] => Array ( [image__header_ads2] => [subtitle_text__header_ads2] => [title__header_ad_ads2] => [sub_title_2_ads2] => [content__text__ad_ads2] => [link__header_ads2] => ) [announcement_box] => [ID] => 1106 [title] => Cell perfusion pack [permalink] => https://www.elveflow.com/microfluidics-application-packs/biology-packs/perfusion-for-cells-and-biology/ [post_type] => packs [post_type_name] => Application pack ) [1] => stdClass Object ( [preview] => Array ( [image] => Array ( [ID] => 85378 [id] => 85378 [title] => Mechanobiology pack Elveflow confiner Cobalt [filename] => Mechanobiology-pack-Elveflow-confiner-Cobalt.webp [filesize] => 29636 [url] => https://www.elveflow.com/wp-content/uploads/2022/04/Mechanobiology-pack-Elveflow-confiner-Cobalt.webp [link] => https://www.elveflow.com/microfluidics-application-packs/biology-packs/cell-confinement-pack/mechanobiology-pack-elveflow-confiner-cobalt/ [alt] => Mechanobiology pack Elveflow confiner Cobalt [author] => 27 [description] => Mechanobiology pack Elveflow confiner Cobalt [caption] => Mechanobiology pack Elveflow confiner Cobalt [name] => mechanobiology-pack-elveflow-confiner-cobalt [status] => inherit [uploaded_to] => 81813 [date] => 2022-05-23 10:12:53 [modified] => 2023-12-15 16:42:25 [menu_order] => 0 [mime_type] => image/webp [type] => image [subtype] => webp [icon] => https://www.elveflow.com/wp-includes/images/media/default.png [width] => 1000 [height] => 618 [sizes] => Array ( [thumbnail] => https://www.elveflow.com/wp-content/uploads/2022/04/Mechanobiology-pack-Elveflow-confiner-Cobalt.webp [thumbnail-width] => 150 [thumbnail-height] => 93 [medium] => https://www.elveflow.com/wp-content/uploads/2022/04/Mechanobiology-pack-Elveflow-confiner-Cobalt.webp [medium-width] => 300 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[is_best_seller] => [subtitle] => Cell confinement pack ) [avantages] => Array ( [liste] => Array ( [0] => Array ( [icon] => icon-arrow-right [titre] => Mechanobiological cell response [texte] => Allows advanced molecular and morphological analysis of confined cells ) [1] => Array ( [icon] => icon-arrow-right [titre] => Precise mechanical stimulus [texte] => Trigger mechanical stimulus by adjusting confinement in real time ) [2] => Array ( [icon] => icon-arrow-right [titre] => Standard cell culture plate [texte] => Compatible with 35 mm petri dishes and flat surfaces ) [3] => Array ( [icon] => icon-arrow-right [titre] => Easy to use [texte] => Fast experiment set-up with plug-and-play equipment ) ) ) [introduction_texte] => Elveflow® provides a perfusion system dedicated to cell culture, for lab-on-chips, flow cells and perfusion chambers. This pack includes all the necessary elements to create a continuous flow and monitor flow rate applied on the cells. Ideal for experiments requiring switches between different cell culture media. A computer-controlled valve allows sequential injections (up to 12 different medium or reagents, more on demand). The intuitive ESI control software allows to quickly automate your complex experimental workflows. 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[alt] => Mechanobiology pack Elveflow cell confiner microscope2 [author] => 27 [description] => Mechanobiology pack Elveflow cell confiner microscope2 [caption] => Mechanobiology pack Elveflow cell confiner microscope2 [name] => mechanobiology-pack-elveflow-cell-confiner-microscope2 [status] => inherit [uploaded_to] => 81813 [date] => 2022-05-23 10:12:53 [modified] => 2023-12-15 16:42:24 [menu_order] => 0 [mime_type] => image/webp [type] => image [subtype] => webp [icon] => https://www.elveflow.com/wp-includes/images/media/default.png [width] => 1000 [height] => 618 [sizes] => Array ( [thumbnail] => https://www.elveflow.com/wp-content/uploads/2022/04/Mechanobiology-pack-Elveflow-cell-confiner-microscope2-150x150.webp [thumbnail-width] => 150 [thumbnail-height] => 150 [medium] => https://www.elveflow.com/wp-content/uploads/2022/04/Mechanobiology-pack-Elveflow-cell-confiner-microscope2-300x185.webp [medium-width] => 300 [medium-height] => 185 [medium_large] => 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A standard setup contains a pressure controller and a cell confiner to better mimic in vivo cell culture conditions. The Cobalt pressure controller from Elveflow generates stable pressure while the cell confiner from 4D Cell provides micrometric control of the culture microenvironment. The combination of cell compression and spatial control provides insight into cell behavior in confined spaces and under mechanical stress, increasing the translational value of in vitro cell-based assays. Advantages for mechanobiology studies Control both pressure & physical space: Ideal for mechanical stress assays. Allows fast switches between pressure settings: For real-time observation of cell responses to mechanical stimulus. Stable & pulseless pressure: Total and stable control over cell compression. Non-destructive cell assay: Retrieve your cells for molecular analysis. Instantaneous start and stop pressure: Rapid trigger and release confinement. Our dedicated pack can be adapted for more complex and advanced experiments. Contact us for more information! Contents of the Cell confinement pack 1 x Cobalt autonomous microfluidic pump 1 x Security tank 3 x 4Dcell Confiner (PDMS suction cup) PDMS ring-shaped stencil – delimits the seeding area of cells to the region where confinment is applied 12 x Confinment slides: 10 mm diameter glass cover slips containing the PDMS micro-structures (pillars) Confinement heights from 1 to 20 µm (you may select up to 3 heights for your kit) 1 x Tubing and connectors kit [image] => ) [applications] => Array ( [texte] =>
Elveflow® provides a perfusion system dedicated to cell culture, for lab-on-chips, flow cells and perfusion chambers. This pack includes all the necessary elements to create a continuous flow and monitor flow rate applied on the cells.
Ideal for experiments requiring switches between different cell culture media. A computer-controlled valve allows sequential injections (up to 12 different medium or reagents, more on demand).
The intuitive ESI control software allows to quickly automate your complex experimental workflows.
A standard Cell & Biology Pack setup uses one pumping channel to flow multiple solutions into the microfluidic chip. In combination with the flow sensor (MFS or BFS series), the OB1 flow controller allows to have a very stable medium perfusion. Moreover, the medium change is easily done using the MUX Distribution that allows to switch between 12 solutions. All these operations can be performed using only the ESI software interface. The software allows you to fine tune the flow parameters as well as to automate your experiment using the intuitive scheduler.
Our dedicated pack can be adapted for more complex and advanced Cell & Biology experiments such as using 20 solutions, choosing the right microfluidic chip, removing bubbles or to have multiple chip/inlets perfusion.
Contact us for more information!
To help you determine your flow rate, pressure to apply, the best tubing resistance length for your setup, wall shear stress for biology applications, cell culture, and many more…
Elveflow provides its microfluidic calculator and…to make the most of our microfluidic calculator, find below a set of dedicated application notes:
Learn more on dynamic cell culture using this pack.
Learn how to automate cell seeding with this pack.
Learn more on long-term medium perfusion using this pack
The amazing benefits of microfluidics can be applied to many Cell & Biology perfusion experiments and applications. Therefore, the content of the Cell & Biology Pack can be adjusted to suit your specific needs. Contact us for more details.
We combined the Elveflow pressure control technology with the state-of-the art 4D Cell Confiner to create the ideal experiment conditions for biomechanical analyses of cells and tissues.
The Mechanobiology pack brings together 4Dcell‘s cell confinement technology with the best in pressure control from Elveflow.
A standard setup contains a pressure controller and a cell confiner to better mimic in vivo cell culture conditions. The Cobalt pressure controller from Elveflow generates stable pressure while the cell confiner from 4D Cell provides micrometric control of the culture microenvironment.
The combination of cell compression and spatial control provides insight into cell behavior in confined spaces and under mechanical stress, increasing the translational value of in vitro cell-based assays.
Our dedicated pack can be adapted for more complex and advanced experiments. Contact us for more information!
A complete microfluidic system for Organ-On-Chip experiments. Perform lung on a chip, kidney on a chip, gut on a chip… straight out-of-the-box! Dive into the cutting-edge field of Organ-On-Chip with Elveflow’s Pack!
Based on our best-selling OB1 pressure-driven flow controllers, this fully integrated solution contains all the required microfluidic parts for researchers to reproduce numerous characteristics of the in vivo environment of cells and tissues.
Elveflow’s Organ-On-Chip Pack setup uses one flow controller to flow a medium through a chip containing the organ cells. The software allows a very accurate and stable control of the flow rate over defined periods of time.
Whether you want to add multiple fluidic channels, customize your microfluidic chip or add a vacuum control, our microfluidic specialists can customize the pack so that it can best fit your experimental needs.
Organs-on-chips applications do not only have several advantages such as miniaturization, integration and low consumption, but also they allow researchers to accurately control the multiple parameters of a system such as chemical concentration gradient, fluid shear stress, cell patterning, tissue-tissue interface, organ-organ interaction and so on. They aim at mimicking the complex structure, microenvironment and physiological function of human organs.
These applications hold the promise of having a significant impact on improving the predictability of drug screening models and personalized medicine. Organ-on-a-chip technology supports these research areas by providing an environment that can mimic the human physiology and morphology in vivo better than traditional static cell culturing methods. Scalable organ-on-a-chip production is made possible by combining the technologies originating from both the semiconductor and molecular biology industries.
The Organ-On-Chip Pack can be used with any commercial chip or self-made chip.
ChipShop is a company offering versatile chips for several applications including 3D cell culture and organ-on-a-chip. Particularly, the cross flow membrane chip (Fluidic 480) enables to create a tissue interface to mimic organs functions (gut-on-chip, brain-on-chip, kidney-on-chip, skin-on-chip, etc.).This chip hosts two different culture chambers separated by a permeable membrane. Different cell types can be seeded on one or both sides of the membrane and exposed to dynamic flow conditions.
Microchip with 2 independant chambers for organ on chip studies
There are several chip designs available for organ-on-chips experiments, depending on the type of organs that scientists would like to mimic and the experimental protocols. Elveflow is working closely with several organ-on-chips providers to supply the most suitable chip for your science.
A microfluidic circulatory system integrated with capillary-assisted pressure sensors
Lab on Chip, Y. Chen, H. N. Chan, S. A. Michael, Y. Shen, Y. Chen, Q. Tian, L. Huang and H. Wu, 2017
Bioengineered Human Organ-on-Chip Reveals Intestinal Microenvironment and Mechanical Forces Impacting Shigella Infection
Cell Host & Microbe, Alexandre Grassart et al., 2019
A Fast Alternative to Soft Lithography for the Fabrication of Organ-on-a-Chip Elastomeric-Based Devices and Microactuators
Science Advances, Daniel A. Ferreira et al., 2021
Elveflow’s expert in liquid actuation & flow control recommend the use of relevant chips such as the MesoBioTech solutions with these advantages:
Elveflow’s Recirculation Pack is a complete system which enables automatic re-use and unidirectional recirculation of liquids in microfluidic experiments.
Using Elveflow’s MUX Recirculation 6/2 valve, this pack brings the many benefits of our technology such as pulseless smooth flow, reproducibility, accurate and precise flow rate control for cell culture experiments. It enables full automation of week-long experiments with limited media volumes or more advanced applications such as the modeling of complex biological flow patterns.
A standard Recirculation Pack setup uses two pumping channels to alternatively flow medium from one reservoir to another. An injection valve allows to switch between both reservoirs, to ensure that the medium is pumped from the most filled reservoir. The injection loop consists of a network of inputs/outputs that can be connected to each other in two configurations. Its very low internal volume (2.5 μL) makes it the ideal valve to perform the injection of a controlled volume of sample into a chip.
The Elveflow® smart interface (ESI) synchronizes the flow controller together with the injection valve in order to perform recirculation. It performs the switch between reservoirs automatically either after a predetermined period of time or, in combination with a the flow rate sensor (MFS), after a chosen amount of sample has gone through the chip. The software is also able to seamlessly run long term experiments thanks to its powerful scheduler.
Additional features such as medium switch, one-off sample injection, etc… are also possible by adding extra Elveflow’s equipment to your Recirculation Pack.
Contact us for further information!
Adaptable pulsatile flow generated from stem cell-derived cardiomyocytes using quantitative imaging-based signal transduction
T. Qian et. al, Lab on a Chip, 2020, 20, 3744-3756, DOI: 10.1039/D0LC00546K
Investigation of flow dynamics and structure in channels with structured surfaces
P. Vilkinis, 2020, Doctoral Thesis, Kaunas University of Technology, Lituanian Energy Institute
A standard Recirculation Pack contains the following:
An all-inclusive Pack to generate droplets out-of-the box. We setup a dedicated set of instruments, controlled by a unique software, to ease the droplet generation process.
It brings the many benefits of microfluidics, such as excellent monodispersity, reproducibility and scalability to your daily work in order to achieve great science.
It has never been easier to start generating droplets following a step-by-step User Guide and quickly master the process with the same system.
Schematics outlining multiplexed RNA and protein detection methods by Mayr U. et al.
This all-included, user-friendly, customizable, and automatable instruments pack is ideal for MERFISH/seqFISH/seqFISH+ experiments but can be easily adapted to other applications.
The pack is compatible with different instruments, and the entire setup is controlled via a single software.
This pack includes our premium Elveflow product line and our best-seller, the OB1 flow controller. Thanks to the high performance of this equipment, you will be able to:
*The sequence scheduler automatizes the platform to flow a large number of different solutions easily. Our experts can help you integrate your experiment using TTL triggers or direct software integration via SDK.
This pack is designed to be:
It allows you to perform multiplexed fluorescence in situ hybridization at a microfluidic scale, significantly decreasing the cost of each experiment by reducing the volume of the reagents required.
Elveflow microfluidic platforms are perfectly suited for long-term experiments with excellent stability and no risk of potentially harmful pressure spikes.
All the pack items are adjustable to your laboratory infrastructure and experimental requirements.
Fig 1: This is an illustrative setup example. The solutions’ number and volume can be adjusted according to the experimental requirements and protocol.
seqFISH is a highly sensitive technique that accurately detects low-copy number genes often not detected with single-cell RNAseq or immunostaining. Additionally, reverse transcription or PCR amplification often biases quantification in RT-PCR and RNA sequencing. Because seqFISH can be applied to any tissue type without preselecting genes, it enables the unbiased discovery of novel genes relevant to certain biological phenomena.
seqFISH generates accurate in situ quantification of mRNA levels [1]
SeqFISH and MERFISH use probe detection for single-cell spatial transcriptomics [1][2][3].
At first, in situ hybridization is done with one set of fluorescent FISH probes and a labeling dye. DNase is then used to remove the fluorophores, and the mRNA is hybridized with the identical FISH probes again but with a different labeling dye. The several rounds of hybridization and other dyes allow the barcoding of several genes in a single cell [4].
SeqFISH+ is the improved seqFISH technique ideal for spatial and biological processes studies of cells. It combines seqFISH with a confocal microscope generating super-resolution imaging and multiplexing of 10,000 genes in a single cell [5].
Multiplexed Error Robust Fluorescence In Situ Hybridization (MERFISH) is the improved single-molecule Fluorescence In Situ Hybridization (smFISH). The method massively parallelizes and simultaneously spatially identifies hundreds of thousands of RNA species. In addition, this method detects errors that can then be corrected in an error-robust way thanks to the use of some unassigned binary barcode. This is the main difference compared to seqFISH, which is coded in a color sequence [6].
Lab-on-chip technologies and microfluidic platforms improve seqFISH and MERFISH methods by reducing the cost and the experiment time, providing automation to the process, and improving reproducibility [7].
*Chips are available in several materials, heights, widths, lengths, and shapes, with different numbers of inlets/outlets, and are compatible with different FISH methods. We can advise you on different chips to fit this pack.
Build your pack in three quick and easy steps:
Using microfluidics is the most efficient method to perform MERFISH (Multiplexed Error-Robust Fluorescence In Situ Hybridization) or seqFISH (sequential Fluorescence In Situ Hybridization) and observe multiple genes and their spatial configuration because:
This pack can be combined with other microfluidic steps before this fluorescent in situ hybridization setup. For example, for single-cell isolation, you can use microfluidic single cell encapsulation [1].
Microfluidics can also be used for the method called MA-FISH, which uses oscillatory flows of diluted probe solutions or to perform barcoding (DBiT-seq).
Elveflow has been the microfluidic flow control expert company for more than 10 years and can provide its state-of-the-art expertise in biology and engineering, thus, being the perfect partner for you to transition to microfluidics.
Schematics outlining multiplexed RNA and protein detection methods. [1]
[1] Mayr U., Serra D., Liberali P. Exploring single cells in space and time during tissue development, homeostasis and regeneration. Development, 2019, 146(12),
Why Choose Microfluidics in Education?
Microfluidics offers significant benefits such as low sample and reagent consumption, high reproducibility, precise experimental control, easy automation, and continuous processing. This makes it an invaluable tool for teaching and research in various scientific fields.
Get in Touch
For more information or to discuss your specific educational needs, contact our experts. Let us help you bring the best of microfluidics to your classroom.
At small scale, liquid handling can be controlled precisely with the help of microfluidics.
This all-in-one system for the sequential injection of liquids/medium is based on two centerpieces: a pressure controller and a rotary valve.
The pressure-driven flow controller allows higher stability, better response time compared to systems using a peristaltic pump or a syringe pump,
This liquid handling pack is simple to set-up and to operate and enables easy system automation.
The platform can be adapted to any requirements and fully automated.
Our liquid handling pack enables to sequentially inject different liquids. It contains: a MUX Distribution bidirectional valve which works as a selector, a high accuracy flow controller, and Elveflow’s intuitive ESI control software.
The pack also contains a manifold to pressurize several reservoirs using a single pressure channel from the OB1 and a flow sensor to monitor and control the flow rate in real-time.
The MUX distribution rotary valve is characterized by:
If your experiment requires the use of a buffer in large quantities but also expensive reagents in tiny volumes, it is not an issue for our liquid handling pack. You can actually connect tanks with various volumes, from bottle to Eppendorf.
Moreover, there is no restriction concerning the use of liquids as the wetted materials of the system are resistant to aggressive reagents. The flow sensors can also be chosen to work with liquids of different properties.
More information on how to perform a stable and precise flow switch in our example application note.
Our liquid handling pack can be adapted for more complex and advanced experiments such as using 20 or more solutions, removing bubbles, integration into larger systems or testing multiple chip/devices simultaneously…
Contact us to know more!
To discover more tips and tricks…
Get in touch to claim our complete Userguide about Liquid handling & Sequential Injection!
The liquid handling pack can be used in various fields of application and for any experiment that require successive injection of liquids:
For more biology-related content, please refer to the cell perfusion application pack.
T. Qian et al., Lab on a Chip, 2020, 20, 3744-3756, DOI: 10.1039/D0LC00546K
Microfluidic platform for 3D cell culture with live imaging and clone retrieval
C. Mulas et al., Lab on a chip, 2020, 20, 2580-2591, DOI: 10.1039/D0LC00165A
The amazing benefits of microfluidics can be applied to almost any application and therefore the content of the liquid handling pack can be adjusted to suit your specific needs. Contact us for more details.
Selecting the best flow control method for your application is very important, since the performance and fluid handling efficiency of a microfluidic device depends highly on the flow control system. If you require to use our pack for the previously mentioned applications, the solution will contain:
You can choose from the following additional options:
Alginate beads production has never been that easy using our all-in-one hydrogel particle generation system!
Based on our high accuracy OB1 flow controllers, this all-in-one solution contains all the required parts for researchers to set up their own alginate beads generation system out of the box. It brings the many benefits of microfluidics, including excellent monodispersity, sphericality, reproducibility, and high throughput compared to other hydrogel particle production techniques.
A standard alginate beads generation pack contains one pumping channel to flow the aqueous alginate phase and another pumping channel to push the continuous oil phase through our droplet generation chip, enabling the generation of alginate droplets in oil.
The droplet size will be determined by the chip channel size and the flow rate ratio of both phases. Flow rates can be measured thanks to our multiple flow rate sensors (MFS or BFS series).
For the detailed experimental steps, please refer to our application note!
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Droplet microfluidics as a tool for production of bioactive calcium phosphate microparticles with controllable physicochemical properties, P. Habibovic et al., Acta Biomaterialia (2021).
3D flow-focusing microfluidic biofabrication: One-chip-fits-all hydrogel fiber architectures, Rui L. Reis et al. , Applied Materials Today (2021).
Additive batch electrospinning patterning of tethered gelatin hydrogel fibres with swelling-induced fibre curling
Elisabeth L. Gill, Wenyu Wang, Ruishan Liu, Yan Yan Shery Huang, Additive Manufacturing, 2020, Volume 36, 101456, DOI: 10.1016/j.addma.2020.101456
Highly Ordered Gelatin Methacryloyl Hydrogel Foams with Tunable Pore Size
The amazing benefits of microfluidics can be applied to many droplet applications and therefore the content of the alginate pack can be adjusted to suit your specific needs. Contact us for more details.
Microfluidics brings reproducibility and control over particle or droplet size that is not achievable by any other technique. Continuous hydrogel beads generation with constant size and flow rates control, results in highly monodisperse particles (PDI < 5%).
Moreover, microfluidics is an ideal technique for handling ultra small volumes and control what goes into each droplet or bead. Microfluidics give you flexibility to easily generate identical carrier with the same content. For more detailed protocole about alginate beads generation, please refer to our application note!
Elveflow offers a specific chip that allows you to generate a broad of range of beads size, shape and materials. For more information, please refer to our application note about alginate beads generation.
If you’re unsure about the settings that are best suited for your application, get in touch with one of our experts!
Are you working with small samples or are you looking into manufacturing 100x mLs of hydrogel beads solution? Elveflow offers a comprehensive range of reservoirs compatible with our OB1 flow controller, from 1.5 mL Eppendorf tubes to 100mL bottles.
To make monodisperse and stable alginate beads, Elveflow offers a range of oils and surfactants. For more detailed information, please refer to this application note!
Hydrogel-based delivery systems are finding more and more applications, from encapsulation, protection, to the release of bioactive agents in many fields such as pharmaceutical, supplements, food industries, for cell/bacteria/microbes culture & implantation, and cell-based genes in biological research.
Are you interested in implementing hydrogel beads production in your lab?
Contact us to have your dedicated your dedicated hydrogel beads generation pack that suits your application needs!
Highly monodispersed alginate droplets, size in the 50µm range. Experiment results by the Elveflow team.
Do you want to implement droplet-based microfluidics? We designed a comprehensive solution based on our high precision instruments set. This Pack includes all you need to understand the droplet generation process from 0 to 1.
Start from scratch and quickly master all key parameters triggering and controlling droplet generation.
Droplet forming inside the microfluidic chip
Microfluidics brings reproducibility and control over droplet size that are not achievable with any other technique.
Moreover, it is an ideal technique to handle ultra small volumes and control droplet generation precisely. It give you flexibility to easily generate identical droplets with the same content or to create individual droplets with unique payloads.
This Droplet Generation Pack includes our OB1 flow controller best-seller.
Thanks to the performance of this instrument, you will be able to generate:
We can provide you with two Droplet Pack versions : the small version, to generate smaller droplets ranging from 10 to 40µm and the large version, for droplets ranging from 50 to 80µm diameter. Each Pack is composed of:
All our application notes
All our reviews
And more!
Our two versions only differ by the flow rate range of the oil phase.
Important note: our products are all intercompatible and you may acquire additional flow rate sensors or add extra products for specific applications. You will still benefit from the centralised ESI software control.
Elveflow developped a microfluidic calculator that allows you to estimate all the key parameters at stake in your microfluidic system. Find out more below !
The microfluidic chip is where the two immiscible phases (water and oil here) are precisely injected and mixed to generate monodisperse droplets (CV < 3%). The two microfluidic chips are made of Topas. Topas is a cyclic olefin copolymer (COC) resin which is a chemical relative of polyethylene and other polyolefin plastics.
Are you working with small samples or are you looking into manufacturing 100x mLs of emulsion? Elveflow offers a comprehensive range of reservoirs compatible with our OB1 Flow Controller, from 1.5 mL Eppendorf tubes to 100mL bottles.
Flow resistances are used to increase the resistivity of the microfluidic system to improve the stability and control of the flow rate in the system.
Simple and intuitive instructions are provided to quickly and easily make droplets and control droplet generation parameters. When starting out, the user can follow step by step the provided protocol to obtain droplets of the specific size. In a second stage, the user can rely on the numerous tips provided and explore the “going further” section to complete its training in droplet generation and microfluidic flow control.
Everything you need to start running microfluidics experiments right away.
Elveflow’s Starter Pack contains all necessary elements for you to start your own microfluidic experiments. Based on Elveflow’s popular OB1 Flow Controller, this easy-to-use system covers the majority of microfluidics researchers’ needs. It is fully compatible with the whole Elveflow product range, enabling you to upgrade your system as your needs grow.
From simple single-channel microfluidic flow to multi-channel droplet experiments, the Starter Pack covers hardware needs for a wide range of applications. The whole system is controlled by the powerful ESI software which allows you to simply set pressures and monitor your experiment, or try advance functions such as full automation and running scripts.
You can add different Elveflow modules to suit your experimental needs. These modules include valves, sensors, bubble traps and many more.
Experiments performed with the microfluidics Starter Pack
Microfluidics can be applied to lots of varied applications and therefore the content of the Starter Pack can be tweaked to suit your specific needs.
A standard starter pack contains the following:
Microfluidics is the science of manipulating and controlling fluids, usually in the range of microliters (10-6) to picoliters (10-12). It is an amazing technology that brings lots of benefits to many varied applications areas of science such as:
Pressure-driven flow controlled microfluidics: Switch from an accurate pressure control to an effective flow rate control by adding Elveflow’s Flow Rate Sensors.
Those sensors, combined with the ESI software and its built-in feedback loop control, enable monitoring and control of liquid flow rates. You can dial in a flow rate value and your system will quickly and accurately reach that flow rate by adjusting the system pressure.
Elveflow offers a variety of chips designed for specific applications such as droplet generation, flow focusing, cell encapsulation, and many more.
The OB1 Flow Controller is a powerful instrument that can be tailored to suit your needs. We offer different channels, for vacuum and/or pressure, from -900 mBar up to 8000 mBar. You can swap or add channels to your OB1 Flow Controller at a later date. Contact us to find out more about the procedure.
An all-in-one flow control system for fast switching of fluids while maintaining a defined flow rate.
Several experiments or tests can be carried out with one system at once so as to automate your multiple liquid injection / testing and save time!
Elveflow’s sequential fluid injection pack is dedicated to any system that requires to quickly swap between several fluids while maintaining a precise flow rate. This makes it a perfect fit for your complex organic chemistry reactions, organic synthesis, biosensors, biochemical sensors or electrochemical sensors, test rigs, flow chemistry, Seq-Fish experiment, analytical chemistry setup, drug and toxicity testing, particle synthesis, organocatalysis experiment, mother machine for antibiotic resistance testing and many more…
This pack is suitable for both biological and chemical experiments such as multiple perfusion switch of liquid media and automated sequential reagent injection.
This pack includes all the necessary elements to sequentially inject up to 12 (or more on request) solutions in a fully automated fashion using our computer-controlled 12 to 1 MUX Distribution bidirectional valve, high accuracy OB1 flow controller, and intuitive ESI control software.
A large flow rate range (from 7 nL/min to 30+ mL/min) and volumes (100 µL to up to several Liters) are accessible with this system.
The typical Sequential fluid injection pack setup uses only one single pressure channel to sequentially flow multiple solutions into your microfluidic system. Medium change can then easily be carried out using the MUX Distribution‘s rotary valve that acts as a liquid selector and allows fast switching and selection between your solutions.
In combination with an MFS or BFS flow sensors, the OB1 flow controller allows an extremely stable liquid perfusion (down to 0.006% of the flow rate).
All these operations can be performed using the ESI software interface. The software allows you to fine-tune the flow parameters as well as to automate your experiment using our intuitive scheduler.
Our sequential fluid injection pack can be adapted for more complex and advanced experiments such as using 20 or more solutions, removing bubbles, integration into larger systems or testing multiple chip/devices simultaneously…
Get in touch to claim our complete Userguide about Automated Fluid Sequential Injection!
The sequential fluid injection pack can be used in various fields of application and for any experiment that require successive injection of fluids: from organic synthesis, flow chemistry, drug screening, biochemical and electrochemical sensor calibration, calibration (bench testing), sensors test rig, toxicity test, Seq-Fish experiment…
The amazing benefits of microfluidics can be applied to almost any application and therefore the content of the Sequential fluid injection Pack can be adjusted to suit your specific needs. Contact us for more details.
The Lipid Nanoparticle Synthesis Pack includes all the parts needed to easily synthesize your lipid nanoparticles with high monodispersity, production rate and reproducibility for the optimal encapsulation of your mRNA or siRNA molecules.
Microfluidics presents tremendous advantages to overcome the issues encountered with common Liposome bulk preparation. Being characterised by laminar flow conditions and diffusive mass transfer, microfluidics allows for the production of liposomes with excellent control over size and lamellarity. In addition, it enables in situ monitoring of lipid nanoparticles formation process, continuous production and straighforward scale up.
Based on our high accuracy OB1 flow controller, this solution reduces lipid nanoparticle synthesis time and allow to work at small and large volumes (µL to L) while optimizing reagent consumption.
The lipid nanoparticle generation Pack offered by Elveflow is designed for researchers with no experience in microfluidics and/or lipid nanoparticle generation to easily produce lipid nanoparticles (LNP) using microfluidics techniques.
Thanks to our pressure-driven flow control system, a wide range of flow rates (TFR) – from µL/min to 10s of mL/min – can accommodate both low (µL) and large (L) volume production.
This versatile platform can be easily scaled up to meet your requirements from the screening to the production stage.
Lipid nanoparticle (LNP), solid lipid nanoparticles (SLN) and nanoliposomes can be synthesized using this instrument pack.
Historically, the generation of LNPs was carried out using standard bulk processes (precipitation, emulsion, solvent evaporation and sonication). However, these techniques suffer from broad size distribution and poor batch-to-batch reproducibility. This is highly problematic in clinical trials and production stages of drug development. Microfluidics is a highly promising alternative that has attracted a lot of attention for its advantages as a LNP fabrication method.
Some of these advantages include:
For more information you can read our review about nanoparticles synthesis in microfluidics.
We have conducted extensive characterisation of LNPs generated using this pack including DLS and cryoTEM measurements. Using various TFR and FRR conditions, we synthesised LNPs ranging from 60 up to 250 nm diameter.
Intensity (yellow), number (green) and volume (blue) profiles of LNPs generated using the Herringbone micromixer with a 3:1 FRR and a 4ml/min TFR.
Plot of ImageJ analysis of LNPs size generated using the Herringbone micromixer with a 3:1 FRR and a 4ml/min TFR.
Mixing the two aqueous/RNA and ethanol/lipid phases is the key step of NPs generation. In this Pack, based on microfluidics efficiency, this is done using micromixing chips. For instance, one would use a flow focusing micromixer allowing for passive nucleation of nanoparticles.
This Pack and our experts help you choosing the appropriate chip!
Ribonucleic acid (RNA) is a critical polymeric molecule for the regulation and expression of genes. RNA interference (RNAi) is a method that silences genes by using sequence-specific small interfering RNA (siRNA). Based on their mRNA counterpart nucleotide sequences siRNAs block the production of specific proteins [1-3]. To deliver siRNA for therapeutic applications, lipid nanoparticles (LNP) are the most commonly used system for in vivo applications like anti-tumor agent or polyneuropathies treatment [4-5]. The BioNTech/Pfizer’s BNT162b2 and Moderna’s mRNA-1273 vaccines also use lipid nanoparticles as vehicles for mRNA delivery into the cytoplasm of host cells that lead to the production of COVID-neutralizing antibodies [5]. Thus, lipid nanoparticle synthesis have played a critical role in the development of COVID-19 vaccines and other nanomedicines and are considered to be very promising for the development of new drug delivery systems [6].
Lipid nanoparticles can be used for other applications like using solid lipid nanoparticle (SLN) as cosmetic delivery systems [7].
The lipid nanoparticle synthesis pack includes staggered herringbone micromixers that create chaotic flows. They are the most commonly used microfluidic chips for LNP synthesis [8]. The structure generates chaotic advection which is able to induce rapid mixing at Reynolds numbers smaller than 1 [9] thus allowing a better encapsulation efficiency than bulk methods [10].
Lipid nanoparticle (LNP) formulation example
The amazing benefits of microfluidics can be applied to many Liposome and Lipid Nanoparticle applications and therefore the content of the Liposome and Lipid Nanoparticle Synthesis Pack can be adjusted to suit your specific needs!
There are a few ways to generate lipid nanoparticles in microfluidics. In any case, the most important part relies on the fast mixing of two phases (organic and aqueous). The more efficient and homogeneous the mixing is, the better the control over the size and its distribution. The following figure illustrates how the speed of mixing influences the LNPs size; a slow dilution of the ethanol phase (a) leads to large particles in comparison with a faster dilution (b).
The Herringbone ChipShop Fluidic 187
The Flow focusing ChipShop Fluidic 386
Contact our experts to know which one will be best suited to your requirements!
The Liposome and Lipid nanoparticle synthesis pack is designed to suit your application requirements.
It contains at least two pumping channels to push the two chemical solutions needed to perform the Liposome and lipid nanoparticle synthesis process inside at least one herringbone micromixer chip. Lipid nanoparticle (LNP), solid lipid nanoparticles (SLN) and nanoliposomes can be synthesized using this instrument pack.
Microfluidics chips are used in this system to induce the mixing of your two solutions at a microfluidic scale. The first liquid contains the lipids in ethanol and the second one is the aqueous solution with possibly the hydrophile load that will be encapsulated inside the newly formed LNP such as siRNA or mRNA for example (see the application tab).
Two different chip designs can be provided with this pack, depending on your requirements:
The production can be easily scaled up by increasing the volumes and flow rates, and/or parallelizing several micromixers instead of one, thus increasing the overall throughput of the system while maintaining monodispersity and yield.
The stability and the speed of the reaction directly depend on the flow rates of each fluid and their ratios in the microfluidic channel. The flow is created by the Elveflow OB1 mk3+ flow controller and the flow rates are measured and regulated thanks to flow rate sensors (MFS or BFS series) permitting a very high accuracy and stable flow control. The combination of these instruments is the fastest and most precise microfluidic flow control available on the market which guaranties the best possible LNP monodispersity and reproducibility. Furthermore, the lipid nanoparticle synthesis process can be automated thanks to the software controlling the Elveflow instruments.
Pressure-driven flow control systems are well-suited for Liposome and Lipid nanoparticle synthesis compared to peristaltic or syringe pumps as they offer the most pulsless flow and can be easily adapted for small and large volumes.
The fluidic 187 herringbone chip from microfluidic ChipShop is composed of three separate channels that are 200 µm deep and 600 µm wide. The two inlets for a single channel are 300 µm wide and the single outlet is 600 µm wide. The microfluidic chip is available in polycarbonate (PC) or Zeonor cyclo-olefin copolymer (COP) materials: these materials are optically transparent and harder than the classically used PDMS.
Herringbone micromixer, ChipShop Fluidic187
Alternatively, you can choose a flow focusing micromixer wich will allow for nanoparticle nucleation thanks to diffusion at the interface of the phases.
The microfluidic platform flexibility can be increased by adding a MUX Distribution 12:1 valve after the reservoirs that allows switching between up to 12 different solutions in an instant. This can be, for example, used to quickly change the load of the lipid nanoparticle!
A broad range of reservoirs are compatible with our OB1 flow controller, from 1.5 mL Eppendorf tubes to 100 mL bottles. It is also possible to add pumping channels on the OB1 pressure control pump to increase the number of parallel micromixer channels further.
Contact our experts to answer any questions about this lipid nanoparticle synthesis pack and how it can match your specifications!
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When paired with the OB1 MKIII+ pressure-driven flow controller, the MUX Recirculation 6/2 allows to: Recirculate a fluid in a closed loop Inject small samples in buffers This valve owes its high chemical compatibility to the PCTFE – PTFE material it is made of. This versatile & biocompatible valve makes long-term experiments easier to automate & run. 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=> inherit [uploaded_to] => 32021 [date] => 2020-11-17 09:50:54 [modified] => 2023-12-15 16:35:54 [menu_order] => 0 [mime_type] => image/jpeg [type] => image [subtype] => jpeg [icon] => https://www.elveflow.com/wp-includes/images/media/default.png [width] => 1000 [height] => 618 [sizes] => Array ( [thumbnail] => https://www.elveflow.com/wp-content/uploads/2020/11/XX_MUX_RECIRCULATION_valve_software_B_ELVEFLOW_MICROFLUIDICS-150x150.jpg [thumbnail-width] => 150 [thumbnail-height] => 150 [medium] => https://www.elveflow.com/wp-content/uploads/2020/11/XX_MUX_RECIRCULATION_valve_software_B_ELVEFLOW_MICROFLUIDICS-300x185.jpg [medium-width] => 300 [medium-height] => 185 [medium_large] => https://www.elveflow.com/wp-content/uploads/2020/11/XX_MUX_RECIRCULATION_valve_software_B_ELVEFLOW_MICROFLUIDICS-768x475.jpg [medium_large-width] => 768 [medium_large-height] => 475 [large] => https://www.elveflow.com/wp-content/uploads/2020/11/XX_MUX_RECIRCULATION_valve_software_B_ELVEFLOW_MICROFLUIDICS.jpg [large-width] => 1000 [large-height] => 618 [1536x1536] => https://www.elveflow.com/wp-content/uploads/2020/11/XX_MUX_RECIRCULATION_valve_software_B_ELVEFLOW_MICROFLUIDICS.jpg [1536x1536-width] => 1000 [1536x1536-height] => 618 [2048x2048] => https://www.elveflow.com/wp-content/uploads/2020/11/XX_MUX_RECIRCULATION_valve_software_B_ELVEFLOW_MICROFLUIDICS.jpg [2048x2048-width] => 1000 [2048x2048-height] => 618 ) ) ) [introduction_texte] => ) [principe] => Array ( [texte] => The MUX Recirculation 6/2 valve is a microfluidic 6-ports / 2-positions valve. The MUX Recirculation 6/2 valve, when combined with the OB1 MKIII+ pressure-driven flow controller, allows to: Recirculate a fluid in a closed loop Inject small volume samples in a liquid stream Run & automate long-term experiments! + Experimental versatility with its high bio- & chemical compatibility (PCTFE – PTFE material) The MUX Recirculation valve has 6 inputs and 2 interchangeable configurations shown on the images below. Each input is connected to a neighbour output, either on the right or the left depending on the configuration. Contact our team of experts to get the perfect fit for your experimental needs! How to perform fluid recirculation with the MUX Recirculation 6/2 valve? For more insights on how to recirculate media in a microfluidic chip, please check out our dedicated application pack! Main features & benefits No overpressure Switch between 2 flow configurations in less than 180ms, thanks to the use of a fast & precise valve motor Biocompatible High chemical compatibility, PCTFE and PTFE material can be used with organic solvent. No waste No dead volume & low carryover volume (0.5µL), for the best solution usage efficiency. Easy ¼-28 connection allows for a leak-free operation and quick connection using standard fittings Reproducibility & Reliability Perform identical injections over and over [image] => ) [applications] => Array ( [texte] => The MUX recirculation can be employed for many applications from dynamic cell culture on chip, cell response to medium change, drug screening, toxicity tests, sample injection, stem cells assays, organ on chip, SPR or TIR imaging coupled with microfluidics… and many more! Application notes How to perform fluid recirculation in your microfluidic system? Unidirectional medium recirculation for microfluidic cell culture How to Control microfluidic sample injection An easy guide to do microfluidic perfusion for dynamic cell culture Reviews Microfluidics and its applications: a short review Microfluidics for PLGA nanoparticles synthesis: a review Microfluidics as a tool for drug delivery Publications Adaptable pulsatile flow generated from stem cell-derived cardiomyocytes using quantitative imaging-based signal transduction T. Qian et. al, Lab on a Chip, 2020, 20, 3744-3756, DOI: 10.1039/D0LC00546K Investigation of flow dynamics and structure in channels with structured surfaces P. Vilkinis, 2020, Doctoral Thesis, Kaunas University of Technology, Lituanian Energy Institute For all the publications using our products, please click here. For all the application notes and reviews based on our product, please click here. ) [specifications] => Array ( [texte] => Category Parameter Value Performances Port to port switching time (ms) 180 ms Max recommended pressure 7 bar Internal diameter 0.5 mm Internal volume(1) 2.5µL Carryover volume(2) 0.5 µL Dead volume (3) None Wetted materials PCTFE, PTFE Number of ports 6 Number of positions 2 Operating temperature 5-40°C Operating Humidity 20-70% non condensing Footnote: Volume inside the system from entrance to exit Volume of liquid that will be mixed with the next liquid. It is not stuck, but will be swept next time a liquid passes. 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microfluidic applications. Thanks to our proprietary Piezoelectronic Technology, the OB1 MK3+ is 10 times more stable and up to 10 times faster than other microfluidic flow controllers. 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[texte] => Cutting-edge Microfluidic Flow Controller Designed by scientists for scientists, the versatile and powerful OB1 MK4 pressure controller provides the perfect flow control for all kinds of applications. Whether you need pressure or vacuum, low or high flow rate, for short or week-long processes and experiments, the OB1 MK4 is the ideal instrument for your microfluidic needs. Check out our OB1 animation here! the Best performance in the market: Piezoelectric Technology The Elveflow OB1 MK4 is the only microfluidic flow control instrument worldwide to use piezoelectric regulators. The piezoelectric technology gives you 20 times more precise and 10 times faster flow control than any other flow controller on the market. Customizable & upgradable: 1 Module, up to 4 channels, 5 pressure & vacuum ranges available The OB1 MK4 can be configured according to your needs. In one piece of equipment, you can have up to 4 pressure and/or vacuum channels (and other customized options). If your needs change, the instrument can be upgraded later, in any way you want. get the Fastest flow rate control when paired with a flow sensor Connect the OB1 MK4 to a standard liquid flow rate sensor (MFS) or our premium Coriolis flow sensor (BFS, suited to both liquid and gas) to directly control the flow rate in your chip. The system continuously calculates the pressure and maintains the desired and constant flow rate. Full control software, SDK, and UART communication Single and intuitive software to get started in a few clicks and automate the most complex and long experiments. The SDK libraries allow you to control the OB1 MK4 using your own code while connecting it to other instruments. The MK4 is also equipped with UART communication protocol in addition to the ESI and SDKs control, allowing it to communicate with most control systems, such as Mac, Linux, Arduino, PLC. OEM version available The OB1 MK4 can be used on a bench setup or embedded in your own product. Elveflow has a solution for every step of your research & development. Discover our OB1 MK4 OEM solution. [image] => ) [applications] => Array ( [texte] => Droplet microfluidics Microfluidic Alginate Bead generation Flow Chemistry Polymer synthesis Cell culture: cell perfusion Mixing & sequential injection Organ on a Chip Cell confinement assays Recirculation Lab on a chip Enhance oil recovery Videos Publications For all the publications using our products, please click here. For all the application notes and reviews based on our product, please click here. ) [specifications] => Array ( [texte] => This table summarizes the main specifications of the Elveflow® OB1 MK4 pressure controller. To provide quality & performance, all of our instruments are tested and calibrated independently (response time, stability, repeatability… ) after being assembled and all test results are kept. Non-contractual information, may be changed without notice (1) Max pressure value might vary by +/- 2.5% (2) Pressure stability (standard deviation) measured over the full pressure range with an external high accuracy pressure sensor (Druck DPI150) (3) Time required to reach 5% of the setting point. Depends on the computer operating system (4) Time required to reach 95% of the set point. Volume dependent – Measurement was done on 12 mL reservoir for a set point from 0 to 200 mbar (5) A vacuum source is mandatory for calibration and use of dual channels even if the channels are to be used in pressure only. FLOW CONTROL Flow sensor compatibility Compatible with the whole MFS and BFS range Monitoring and feedback loop flow control available Flow rates From 0,1 µL/min to 500 mL/min (indicative, please refer to the MFS and BFS series) Liquid Compatibility Non contact pump Any aqueous, oil, or biological sample solution. CONTROL & MONITORING Software control Elveflow Smart Interface Windows 7, 8 and 10, both 32 and 64 bit versions supported Software Development Kit Librairies available: Matlab, Python, Labview, C++ Windows 7, 8 and 10, both 32 and 64 bit versions supported Serial/UART communication protocol on request Data management Possibility to log and extract data (.csv): channel and sensor detailed information using ESI Input profiles Possibility to load profiles: ramp, sine, triangle, square or custom Automation Generate step-by-step sequences using the ESI built-in sequencer Load and save custom configurations (.csv) Screen LCD screen showing pressure and sensor from the channel ELECTRICAL CONNECTION USB connection USB B Sensor connection One M8-4 pins connecter available per channel Compatible with Elveflow sensors: MFS, MPS, MFP, MBD Custom analog sensor supply: 5-24VCustom analog sensor readout : 0-10V TTL trigger In and out available 0-5V OTHER Power consumption 12 W (100 V to 240 V – 50 Hz to 60 Hz) Casing dimensions (5) (length x width x height): 240 x 223 x 80 mm Weight 1.4 kg to 2.9 kg Non-contractual information, may be changed without notice [image] => ) [download] => Array ( [fichiers] => ) [faq] => Array ( [items] => Array ( [0] => Array ( [question] => What pressure range should I choose? [reponse] => The performances of the OB1 (for example, the pressure stability) is tied to the full operating pressure range. In order to achieve optimum performance, we recommend our users to choose the smallest pressure range that covers the required experimental pressures. ) [1] => Array ( [question] => Can I achieve direct flow control? [reponse] => By coupling a MFS or BFS flow sensor to the OB1 flow controller, you can use the software to directly control the flow rate by directly inputting the flow rate value. The software uses a PID loop to control the flow rate by setting automatically the pressure to reach the requested flow rate. Doing so, you can benefit of the best of the two worlds to get a fast and precise (pressure driven) volume flow rate (flow sensor). ) [2] => Array ( [question] => Can I connect a Pressure Sensor to the OB1? - [reponse] => Pressure and flow sensors can be connected directly to the OB1 MK4. The sensors can be used in passive mode (for monitoring only) or active mode (to enslave). ) [3] => Array ( [question] => Do I need a pressure and/or vacuum source to operate the OB1 Pressure controller ? [reponse] => Yes, you will need a pressure source to work with the OB1 pressure controller. You can either use the air supply of your lab if there is one, use a compressed air bottle or use an air compressor. Elveflow has selected a compressor that is perfectly fitted for most application using the OB1. If your OB1 has a vacuum channel, you will need a vacuum pump. Elveflow also proposes a selected pump that work perfectly with the OB1. ) [4] => Array ( [question] => How to install the OB1? [reponse] => Click here Unboxing video ) ) ) [more] => Array ( [more_title] => Software [texte] => ESI is Elveflow’s dedicated software interface, built to make experimenter’s life better. It is perfectly adapted for the control of simple and advanced setups and workflow automation. It integrates several modules that make time-consuming and painful tasks so simple. It comes with SDK libraries to control Elveflow system from your own code. The OB1 control window allows to: Visualize the key parameters of each channel: the command pressure the output pressure Visualize sensor output when one is connected to the OB1 (a flow rate sensor on the first channel in the screenshot presented below) Set any pressure or flow rate pattern (predefined or custom) Easily switch in flow rate control mode (sensor mode) Use the pause/play button to preset the parameters on each channel and launch them simultaneously Save configurations to gain time in the future or automate your workflow thanks to the sequencer Open a graph to visualize and save data Launch and use dedicated modules to simplify specific experimental tasks [image] => [video] => ) [outline1] => Array ( [text] => Advantages of Pressure control in microfluidics Fast settling time (down to 40ms) High stability and pulseless flow Possibility to handle fluid volumes of several liters Enable both flow and pressure control when used with a flow meter For more details about Pressure driven flow control, please read this application note. [image] => ) [customization] => Array ( [title] => Get the perfect instrument [] => [image] => Array ( [ID] => 1627 [id] => 1627 [title] => ELVEFLOW PRESSURE CONTROLLER OUTLETS@2x 768x441 [filename] => ELVEFLOW-PRESSURE-CONTROLLER-OUTLETS@2x-768x441.jpg [filesize] => 29701 [url] => https://www.elveflow.com/wp-content/uploads/2019/10/ELVEFLOW-PRESSURE-CONTROLLER-OUTLETS@2x-768x441.jpg [link] => https://www.elveflow.com/?attachment_id=1627 [alt] => ELVEFLOW PRESSURE CONTROLLER OUTLETS@2x 768x441 [author] => 27 [description] => ELVEFLOW PRESSURE CONTROLLER OUTLETS@2x 768x441 [caption] => ELVEFLOW PRESSURE CONTROLLER OUTLETS@2x 768x441 [name] => elveflow-pressure-controller-outlets2x-768x441 [status] => inherit [uploaded_to] => 324 [date] => 2019-10-11 14:34:22 [modified] => 2024-08-19 14:21:20 [menu_order] => 0 [mime_type] => image/jpeg [type] => image [subtype] => jpeg [icon] => https://www.elveflow.com/wp-includes/images/media/default.png [width] => 768 [height] => 441 [sizes] => Array ( [thumbnail] => https://www.elveflow.com/wp-content/uploads/2019/10/ELVEFLOW-PRESSURE-CONTROLLER-OUTLETS@2x-768x441-150x150.jpg [thumbnail-width] => 150 [thumbnail-height] => 150 [medium] => https://www.elveflow.com/wp-content/uploads/2019/10/ELVEFLOW-PRESSURE-CONTROLLER-OUTLETS@2x-768x441-300x172.jpg [medium-width] => 300 [medium-height] => 172 [medium_large] => https://www.elveflow.com/wp-content/uploads/2019/10/ELVEFLOW-PRESSURE-CONTROLLER-OUTLETS@2x-768x441.jpg [medium_large-width] => 768 [medium_large-height] => 441 [large] => https://www.elveflow.com/wp-content/uploads/2019/10/ELVEFLOW-PRESSURE-CONTROLLER-OUTLETS@2x-768x441.jpg [large-width] => 768 [large-height] => 441 [1536x1536] => https://www.elveflow.com/wp-content/uploads/2019/10/ELVEFLOW-PRESSURE-CONTROLLER-OUTLETS@2x-768x441.jpg [1536x1536-width] => 768 [1536x1536-height] => 441 [2048x2048] => https://www.elveflow.com/wp-content/uploads/2019/10/ELVEFLOW-PRESSURE-CONTROLLER-OUTLETS@2x-768x441.jpg [2048x2048-width] => 768 [2048x2048-height] => 441 ) ) [texte_1] => Directly connect a flow sensor Control the flow inside your microfluidic setup By connecting a MFS or BFS Flow Sensor to the OB1 MK4, you enable direct rapid and precise regulation of the flow rate. The PID algorithm of the software reads the flow rate and adjusts the pressure accordingly to reach the target flow rate with speed and precision. [texte_2] => 5 pressure ranges for each channel Choose the pressure range best suited to your need We have several pressure channel options available covering a wide range, up to 8000 mbar (116 PSI) and down to -900 mbar (-13 PSI). Choose the pressure channel that covers your experimental pressure requirements. [texte_3] => Choose between 1 to 4 channels, and more… How many channels do you want? The OB1 MK4 is a compact module that can accommodate up to 4 channels (pressure and/or vacuum). Each module can be upgraded at any time and have channels added or changed. You can control as many channels as you want with one computer. Need more channels on one module (8, 16, 64…. or even more)? Our custom service would design a specific pressure controller that fits your needs. ) [outline2] => Array ( [image] => [text] => ) [produits_associes] => Array ( [0] => 375 [1] => 382 [2] => 1084 [3] => 369 ) [application_packs_on] => [software_on] => 1 [distributors_on] => [services_on] => [citation_author_repeater] => [citation_publication_date] => [citation_keywords_repeater] => [description_GS] => [testimonials_prod] => Array ( [0] => Array ( [testimonial_] => Array ( [testimonial_text] => We are using the device extensively at the moment and I have been quite impressed by its capabilities [testimonial_author] => Dr. Hayden Taylor, University of California, Berkeley, United States [testimonial_product] => OB1 flow controller user ) ) [1] => Array ( [testimonial_] => Array ( [testimonial_text] => I found the systems quite robust and easy to connect and use [testimonial_author] => Martino Chiara - deMello Group, ETH Zurich, Switzerland [testimonial_product] => Droplet pack user ) ) [2] => Array ( [testimonial_] => Array ( [testimonial_text] => The OB1 is performing so well and we are very impressed by its behavior so far. [testimonial_author] => Eric Pedrol Ripoll, Facultat de Quimica, Universitat Rovira i Virgili, Spain [testimonial_product] => OB1 flow controller user ) ) [3] => Array ( [testimonial_] => Array ( [testimonial_text] => The Elveflow OB1 enables us precise pressure and flow control and measurement in various microfluidic and lab scale projects from tissue engineering to membrane filtration analysis. [testimonial_author] => Arne Lüken, Chemical Process Engineering at RWTH Aachen, Germany [testimonial_product] => OB1 flow controller user ) ) [4] => Array ( [testimonial_] => Array ( [testimonial_text] => We are the proud owners of an OB1 we bought about a decade ago. It is still going strong. We would like to ask for a quote for another OB1. [testimonial_author] => Dr. David Tadres, University of California, Santa Barbara [testimonial_product] => OB1 flow controller user ) ) [5] => Array ( [testimonial_] => Array ( [testimonial_text] => After an easy installation, we are now using the OB1 and flow sensors routinely and are very satisfied by both the flow stability and the software's user-friendly interface. [testimonial_author] => Nelson BC Serre and Matyáš Fendrych, Cell Growth Lab, Department of Experimental Plant Biology, Charles University, Czech Republic [testimonial_product] => OB1 flow controller user ) ) [6] => Array ( [testimonial_] => Array ( [testimonial_text] => We have used Elveflow's products for about 9 years now and are big fans, and have made lots of recommendations to friends in Wageningen and the Netherlands. They are so easy to use, reliable and the pricing is sharp. We are very happy customers! [testimonial_author] => Prof. Dr. Joris Sprakel, Physical Chemistry and soft Matter, Wageningen university, Netherlands [testimonial_product] => OB1 flow controller user ) ) [7] => Array ( [testimonial_] => Array ( [testimonial_text] => I've extensively used Elveflow systems in the World's most Brilliant X-ray source. Elveflow systems are precise, controlled and adaptive to many samples under adverse conditions. [testimonial_author] => Pushparani Micheal Raj, PhD, Max Planck Institute of Quantum Optics, Lund, Sweden & MAX IV Laboratory, Bavaria, Germany [testimonial_product] => OB1 flow controller user ) ) [8] => Array ( [testimonial_] => Array ( [testimonial_text] => We use Elveflow instrument in several projects and they are great to work with. The LabVIEW implementation was also very successful. I hope more people can get to know the OB1 system. 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Plug and Play valve system with easy software ) ) ) [introduction_texte] => The Multiplex Flow Matrix is Microfluidic Valve matrix with a custom manifold for a multitude of functions in one easy click Thanks to its integrated manifold and the ROCKER® valve technology, this valve matrix allows you to carry out a multitude of functions in one easy click: instantaneous flow stop, sequences of injection phases, flow focusing, cleaning and stabilizing the flow inside your cross-formed microfluidic chip. 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Connect any of the 4 inputs with any of the 4 outputs and open/close the valves using the software. Zero flow Thanks to the « Zero Flow » function, you will no longer have any residual Poiseuille flow during observation or separation phases within your cross-formed chip. Achieve performance and reproducible results like never before! Low volume injection By using a liquid buffer as a carrier phase, and by introducing your samples using a T Junction or the Elveflow chip holder, the valve matrix allows for the controlled injection of very low volumes (<10µL) into your microfluidic chip. Other instrument highlights include: Plug & Play instrument designed to stop the flow in your microfluidic device in 100ms with no residual flow PEEK valves compatible with 10-32 microfluidic fittings Complete equilibrium & stop flow in 100ms Internal/external trigger [image] => ) [applications] => Array ( [texte] => Sample premixing Fast medium switching – Drug Testing On-chip peristaltic pumping Sequential sample injection Cell/particle sample screening Medium perfusion switch for cell biology Diffusion studies Unidirectionnal recirculation Organ-on-chip Cell culture Publications For all the publications using our products, please click here. For all the application notes and reviews based on our product, please click here. ) [specifications] => Array ( [texte] => • One Trigger IN and one Trigger OUT TTL output. [image] => ) [download] => Array ( [fichiers] => ) [faq] => Array ( [items] => ) [more] => Array ( [more_title] => Software [texte] => ESI is Elveflow’s advanced software interface that is built to make every experimenter’s life better. It is perfectly adapted from simple setups to very advanced setup and workflow automation. It integrates several modules that make time-consuming and painful tasks very simple. Elveflow also provides Software Development Kit (SDK) libraries to integrate Elveflow systems using your own code. ADD your instrument Configure your instrument with just a click Automate your setup and communicate with other instruments using the ESI sequencer Using the ESI interface, you can perform multiple automation functions such as:
Ideal microfluidic valve for precise sample injection and fluid recirculation
The MUX Recirculation 6/2 valve is a microfluidic 6-ports / 2-positions valve. When paired with the OB1 MKIII+ pressure-driven flow controller, the MUX Recirculation 6/2 allows to:
This valve owes its high chemical compatibility to the PCTFE – PTFE material it is made of. This versatile & biocompatible valve makes long-term experiments easier to automate & run.
The MUX Recirculation 6/2 valve is a microfluidic 6-ports / 2-positions valve. The MUX Recirculation 6/2 valve, when combined with the OB1 MKIII+ pressure-driven flow controller, allows to:
The MUX Recirculation valve has 6 inputs and 2 interchangeable configurations shown on the images below. Each input is connected to a neighbour output, either on the right or the left depending on the configuration.
Contact our team of experts to get the perfect fit for your experimental needs!
For more insights on how to recirculate media in a microfluidic chip, please check out our dedicated application pack!
The MUX recirculation can be employed for many applications from dynamic cell culture on chip, cell response to medium change, drug screening, toxicity tests, sample injection, stem cells assays, organ on chip, SPR or TIR imaging coupled with microfluidics… and many more!
Footnote:
The OB1 allows you to control the output pressure of up to 4 channels independently, from -900 mbar to 8 bar, for a wide variety of advanced microfluidic applications.
Thanks to our proprietary Piezoelectronic Technology, the OB1 MK3+ is 10 times more stable and up to 10 times faster than other microfluidic flow controllers.
Designed by scientists for scientists, the versatile and powerful OB1 MK4 pressure controller provides the perfect flow control for all kinds of applications. Whether you need pressure or vacuum, low or high flow rate, for short or week-long processes and experiments, the OB1 MK4 is the ideal instrument for your microfluidic needs.
Check out our OB1 animation here!
The Elveflow OB1 MK4 is the only microfluidic flow control instrument worldwide to use piezoelectric regulators. The piezoelectric technology gives you 20 times more precise and 10 times faster flow control than any other flow controller on the market.
The OB1 MK4 can be configured according to your needs. In one piece of equipment, you can have up to 4 pressure and/or vacuum channels (and other customized options). If your needs change, the instrument can be upgraded later, in any way you want.
Connect the OB1 MK4 to a standard liquid flow rate sensor (MFS) or our premium Coriolis flow sensor (BFS, suited to both liquid and gas) to directly control the flow rate in your chip. The system continuously calculates the pressure and maintains the desired and constant flow rate.
Single and intuitive software to get started in a few clicks and automate the most complex and long experiments. The SDK libraries allow you to control the OB1 MK4 using your own code while connecting it to other instruments. The MK4 is also equipped with UART communication protocol in addition to the ESI and SDKs control, allowing it to communicate with most control systems, such as Mac, Linux, Arduino, PLC.
The OB1 MK4 can be used on a bench setup or embedded in your own product. Elveflow has a solution for every step of your research & development. Discover our OB1 MK4 OEM solution.
For all the publications using our products, please click here.
For all the application notes and reviews based on our product, please click here.
This table summarizes the main specifications of the Elveflow® OB1 MK4 pressure controller. To provide quality & performance, all of our instruments are tested and calibrated independently (response time, stability, repeatability… ) after being assembled and all test results are kept.
Non-contractual information, may be changed without notice
(1) Max pressure value might vary by +/- 2.5% (2) Pressure stability (standard deviation) measured over the full pressure range with an external high accuracy pressure sensor (Druck DPI150) (3) Time required to reach 5% of the setting point. Depends on the computer operating system (4) Time required to reach 95% of the set point. Volume dependent – Measurement was done on 12 mL reservoir for a set point from 0 to 200 mbar (5) A vacuum source is mandatory for calibration and use of dual channels even if the channels are to be used in pressure only.
The performances of the OB1 (for example, the pressure stability) is tied to the full operating pressure range.
In order to achieve optimum performance, we recommend our users to choose the smallest pressure range that covers the required experimental pressures.
By coupling a MFS or BFS flow sensor to the OB1 flow controller, you can use the software to directly control the flow rate by directly inputting the flow rate value.
The software uses a PID loop to control the flow rate by setting automatically the pressure to reach the requested flow rate. Doing so, you can benefit of the best of the two worlds to get a fast and precise (pressure driven) volume flow rate (flow sensor).
Pressure and flow sensors can be connected directly to the OB1 MK4. The sensors can be used in passive mode (for monitoring only) or active mode (to enslave).
Yes, you will need a pressure source to work with the OB1 pressure controller.
You can either use the air supply of your lab if there is one, use a compressed air bottle or use an air compressor. Elveflow has selected a compressor that is perfectly fitted for most application using the OB1.
If your OB1 has a vacuum channel, you will need a vacuum pump. Elveflow also proposes a selected pump that work perfectly with the OB1.
Click here
Unboxing video
ESI is Elveflow’s dedicated software interface, built to make experimenter’s life better. It is perfectly adapted for the control of simple and advanced setups and workflow automation. It integrates several modules that make time-consuming and painful tasks so simple. It comes with SDK libraries to control Elveflow system from your own code.
The OB1 control window allows to:
For more details about Pressure driven flow control, please read this application note.
By connecting a MFS or BFS Flow Sensor to the OB1 MK4, you enable direct rapid and precise regulation of the flow rate. The PID algorithm of the software reads the flow rate and adjusts the pressure accordingly to reach the target flow rate with speed and precision.
We have several pressure channel options available covering a wide range, up to 8000 mbar (116 PSI) and down to -900 mbar (-13 PSI). Choose the pressure channel that covers your experimental pressure requirements.
The OB1 MK4 is a compact module that can accommodate up to 4 channels (pressure and/or vacuum). Each module can be upgraded at any time and have channels added or changed. You can control as many channels as you want with one computer.
Need more channels on one module (8, 16, 64…. or even more)? Our custom service would design a specific pressure controller that fits your needs.
The Multiplex Flow Matrix is Microfluidic Valve matrix with a custom manifold for a multitude of functions in one easy click
Thanks to its integrated manifold and the ROCKER® valve technology, this valve matrix allows you to carry out a multitude of functions in one easy click: instantaneous flow stop, sequences of injection phases, flow focusing, cleaning and stabilizing the flow inside your cross-formed microfluidic chip.
The MUX Cross Chip is a matrix of 4 x 4 valves. Connect any of the 4 inputs with any of the 4 outputs and open/close the valves using the software.
Thanks to the « Zero Flow » function, you will no longer have any residual Poiseuille flow during observation or separation phases within your cross-formed chip. Achieve performance and reproducible results like never before!
By using a liquid buffer as a carrier phase, and by introducing your samples using a T Junction or the Elveflow chip holder, the valve matrix allows for the controlled injection of very low volumes (<10µL) into your microfluidic chip.
Other instrument highlights include:
• One Trigger IN and one Trigger OUT TTL output.
ESI is Elveflow’s advanced software interface that is built to make every experimenter’s life better. It is perfectly adapted from simple setups to very advanced setup and workflow automation. It integrates several modules that make time-consuming and painful tasks very simple. Elveflow also provides Software Development Kit (SDK) libraries to integrate Elveflow systems using your own code.
Using the ESI interface, you can perform multiple automation functions such as:
Flow switch matrix to control Quake (or any other bilayer) PDMS valves
Open and close your Quake Valves in 20 ms. The 3:2 valve matrix coupled with Elveflow software allows you to control the opening and closing of Quake valves in one click. To automate your experiment, you can also program opening and closing sequences in a few easy clicks.
ROCKER® valve technology
With this matrix of 3:2 valves, you can simply choose when to put your PDMS bilayer valve under pressure. When fed by our OB1 MK3+ pressure controller, the 0.005% stability enables fine control and stable valve position.
Our profile editor will allow you to easily program subtle valve patterns and to repeat a set of steps in a loop to automate the most sophisticated protocols.
The MUX Quake offers input/output TTL triggers set to easily synchronize your instrument with any Elveflow® device, microscopes or mechanical shutter. Thus, you can have a full control of all the devices involved in your microfluidic experiment
Using the ESI interface, you can perform multiple automation functions such as: – Save the Quake Valve configuration – Sequence the execution of the Quake Valve with other Elveflow’s instruments using the intuitive scheduler – Synchronize the Quake Valve with other Elveflow or third party instruments using the device’s external/internal TTL triggers
Discover our New 12/1 Distribution Valve.
Perfect microfluidic bidirectional rotary selector valve for handling several samples & automating experiments!
Perform microfluidic sequential injection with the 12/1 Rotary Selector Bidirectional Valve.
Combined with the OB1 MK4 pressure controller, the MUX Distribution valve will allow you to automate injection in microfluidic experiments, program perfusion experiments, sequentially supply different reagents and many more applications.
Several solutions from one outlet or one feed directed into different outlets.
Use the MUX Distribution valve to sequentially inject up 12 different solutions into one microfluidic line for sample collections or parallel experiments.
Bidirectional 13 ports/ 12 positions sequential injection from 12 different samples into 1 output/input
The MUX Distrib’ 12/1 can be used in various fields of application and for any experiment that requires successive injection of fluids: from organic synthesis, flow chemistry, drug screening, biochemical and electrochemical sensor calibration, calibration (bench testing), sensors test rig, toxicity test, Seq-Fish experiment…
Microfluidic Control of Nucleation and Growth of CaCO3
D. K. Dysthe et al. Cryst. Growth Des. 2018, 18, 8, 4528–4535
Add and control 2-way and 3-way valves anywhere in your microfluidic setup
With Elveflow’s MUX Wire, you can plug up to 8 microfluidic valves – either Elveflow’s 2-way or 3-way valves or your own. Each valve is connected individually to the MUX Wire through mini-USB cables that provide power and trigger signal.
The compact design and ease-of-use of the MUX Wire and the valves make them suitable for any microfluidic setups and OEM integration.
The MUX Wire version 3 (V3) microfluidic valve controller works perfectly with all of Elveflow’s microfluidic valves and devices through the same software interface.
It is equipped with internal and external trigger connectors that synchronize it with the other equipment in the setup, such as microscopes or analytical instruments.
The LED indicator on the microfluidic valve controller displays each valve’s type and status, facilitating the use, visualization, and control of all the valves in your setup.
Smart valves can be independently or simultaneously controlled through Elveflow’s software interface (ESI). The ESI automatically detects these valves once plugged into the Mux Wire.
Three microfluidic valve models are available. The valve model relates to the supported pressure – high pressure (until 6 bars), low pressure (until 3 bars), and low volume (for low internal volume requirements).
The valve type indicates the flow path and the normally open (NO) or closed (NC) state of the valve. The V3 range offers three types of valves: 2/2 NO, 2/2 NC, and 3/2 valves.
The combination of valve models and valve types gives different possibilities to fulfill specific setup requirements.
Contact our experts for the valve that perfectly fits your needs!
Elveflow’s smart valves have been carefully selected for their convenience and performance to suit most microfluidic applications. Our valves provide 20ms or less switching time, low internal volume, high chemical compatibility, and no disruption flow.
It is possible to power the MUX wire, plug valves, and control them manually via the push button on the valve. This way, the system works in a standalone mode, without the ESI or a computer!
The 3/2 way valve is ideal for bidirectional control: it can be used to choose between two inlets and one outlet or one inlet and two outlets.
ESI is Elveflow’s advanced software interface that is built to make every experimenter’s life better. It is perfectly adapted from simple setups to very advanced setups and workflow automation. It integrates several modules that make time-consuming and painful tasks very simple. Elveflow also provides Software Development Kit (SDK) libraries to integrate Elveflow systems using your own code.
Some additional features are available when connecting the MUX to the ESI. The new ESI MUX interface offers detailed information about the type and state of the valves connected to the instrument.
Each valve displays:
The smart valves are automatically added once connected to the MUX Wire. A custom valve or an older valve will not be automatically recognized, you will have to add it manually in the software.
The MUX wire V3 allows you to activate your valves in two ways:
Automate your experiment and switch from one configuration to another through the ESI Sequencer:
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It guarantees the best performances on the market, with a perfect reliability. Thanks to our proprietary Piezoelectronic Technology, the OB1 MK3+ is 10 times more stable and up to 10 times faster than other microfluidic flow controllers. 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Our OB1–OEM system takes full advantage of our experience in the field of scientific research, but is also fitted to industrial constraints. Your system will benefit from our high-quality, robust and easy to integrate technology. The performances of a cutting-edge research instrument
Our flow controller OB1 – OEM derives directly from our best seller product, used by hundreds of academics and industrial laboratories worldwide. It guarantees the best performances on the market, with a perfect reliability.
Elveflow is the only company offering cutting edge piezoelectric technology for flow control, and we developed a unique expertise. Our OB1–OEM system takes full advantage of our experience in the field of scientific research, but is also fitted to industrial constraints. Your system will benefit from our high-quality, robust and easy to integrate technology.
The OEM version of the flow controller is designed to be integrated in your complex systems. We have carefully thought of the mechanical and software design aspects in order to perform the integration.
The pressure controller can be customized to fit your needs. Contact us so that we can provide you with the best product for your needs.
This table summarizes the main specifications of the Elveflow® OB1 MK4 OEM pressure controller. To provide quality & performance, all of our instruments are tested and calibrated independently (response time, stability, repeatability… ) after being assembled and all test results are kept.
0.015 % FS / 30 µbar (0.0004 psi)
Development Kit
*Max pressure value might vary by +/- 2.5% (1) Pressure stability (standard deviation) measured over the full pressure range with an external high accuracy pressure sensor (Druck DPI150) (2) Time required to reach 5% of the setting point. Depends on the computer operating system (3) Time required to reach 95% of the set point. Volume dependent – Measurement was done on 12 mL reservoir for a set point from 0 to 200 mbar (4) A vacuum source is mandatory for calibration and use of dual channels even if the channels are to be used in pressure only (5) (length x width x height).
ESI is Elveflow’s more advanced software interface, built to make every experimenter’s life better. It is perfectly adapted from simple setups control to very advanced setups and workflows automation. It integrates several modules that make time-consuming and painful tasks so simple. It comes with SDK libraries (C++, Python, MATLAB, LabVIEW) to control the Elveflow system from with own code.
Our engineers work together in interdisciplinary teams and our project managers will provide you with experienced-based guidance to the final result. By centralizing our expertise, and thanks to an efficient and fast communication, we are able to translate your ideas into a product within a short time frame. We are available for our partners at any time.
Working with us enables you to move resources away from technical developments and focus on innovation.
Benefit from our expertise – Our management is based on senior engineers. We launched up to 15 new microfluidic products in the last 4 years and are used to drive projects with external partners. Our R&D team is familiar with the challenge of industrializing a new product and will provide you with advice and guidance.
A receptive and efficient partner – We are well aware of the importance of keeping up with the fast-changing market you want to address. At Elveflow, we are driven by a reactive and efficient project management. You are ensured that your projects are delivered on time and match your requirements.
A soft intellectual property policy – We believe that intellectual property should never be an obstacle to innovation. With our open-source philosophy, you are able to integrate our OEM solutions to your products and easily patent your innovation.
A trusted manufacturer – We have a proven track record working with high profile companies worldwide.
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These valves have been carefully selected for their convenience and performances to fulfill the needs of most microfluidic applications. We designed a smart interlocking case allowing to use the valves anywhere in your setup a convenient manner. 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controlled through Elveflow’s software interface (ESI). The ESI automatically detects the valves when connected to the Mux Wire V3 valve controller. Three valve models are available. The valve model relates to the supported pressure – high pressure (until 6 bars), low pressure (until 3 bars), and low volume (for low internal volume requirements). The valve type defines the flow path and can have two states by default: normally open (NO) or normally closed (NC) state. The V3 range offers three types of valves:
We provide a wide range of 2-way and 3-way valves to be used with the MUX WIRE. These valves have been carefully selected for their convenience and performances to fulfill the needs of most microfluidic applications. We designed a smart interlocking case allowing to use the valves anywhere in your setup a convenient manner.
Smart valves can be independently or simultaneously controlled through Elveflow’s software interface (ESI). The ESI automatically detects the valves when connected to the Mux Wire V3 valve controller.
Three valve models are available. The valve model relates to the supported pressure – high pressure (until 6 bars), low pressure (until 3 bars), and low volume (for low internal volume requirements).
The valve type defines the flow path and can have two states by default: normally open (NO) or normally closed (NC) state.
The V3 range offers three types of valves:
The MUX Wire version 3 (V3) valve controller can control up to eight valves in your setup. The valves can be independently or simultaneously controlled through the Elveflow Smart Interface (ESI).
It is possible to control the valves manually via the push button on top of the valve. This way, the system works in a standalone mode, without the ESI or a computer!
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Perfect microfluidic bidirectional selector valve for handling several samples & automating experiments! 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[sizes] => Array ( [thumbnail] => https://www.elveflow.com/wp-content/uploads/2012/05/02_MUX_DISTRIB_Distribution_valve_ELVEFLOW_MICROFLUIDICS-150x150.jpg [thumbnail-width] => 150 [thumbnail-height] => 150 [medium] => https://www.elveflow.com/wp-content/uploads/2012/05/02_MUX_DISTRIB_Distribution_valve_ELVEFLOW_MICROFLUIDICS-300x185.jpg [medium-width] => 300 [medium-height] => 185 [medium_large] => https://www.elveflow.com/wp-content/uploads/2012/05/02_MUX_DISTRIB_Distribution_valve_ELVEFLOW_MICROFLUIDICS-768x475.jpg [medium_large-width] => 768 [medium_large-height] => 475 [large] => https://www.elveflow.com/wp-content/uploads/2012/05/02_MUX_DISTRIB_Distribution_valve_ELVEFLOW_MICROFLUIDICS.jpg [large-width] => 1000 [large-height] => 618 [1536x1536] => https://www.elveflow.com/wp-content/uploads/2012/05/02_MUX_DISTRIB_Distribution_valve_ELVEFLOW_MICROFLUIDICS.jpg [1536x1536-width] => 1000 [1536x1536-height] => 618 [2048x2048] => 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Common benefits Several solutions from one outlet or one feed directed into different outlets. Use the MUX Distrib to sequentially inject up 12 different solutions into one microfluidic line for sample collections or parallel experiments. Low internal volume Fast switching time Workflow microfluidic automation Smooth switches: no disruption of the flow Easy setup and operation (plug and play software + standard fluidic connection) Standard 10/1 Valve Bidirectionnal 11 ports/ 10 positions rotary valve where peripheral ports can selectively be connected to a central one. Key advantages: Low internal volume 11.6 uL port to port Fast switching time 280 ms actuation time New 12/1 Valve Bidirectional 13 ports/ 12 positions sequential injection from to 12 different samples into 1 output/input Key advantages: Valve flexibility: 13 ports Easy setup: standard ¼-28 fluidic fittings Possibility to chose the sense of rotation High chemical compatibility Lowest internal volume: 3.5 µL No dead volume Switching time (average): 156 ms Wetted materials: PCTFE, PTFE How to perform microfluidic multiple liquid switching ? For more information on how to perform a stable and precise flow switch, please check our dedicated sequential fluid injection pack! [image] => ) [applications] => Array ( [texte] => The MUX Distribution 10-1 or MUX Distributor 12-1 can be used in various fields of application and for any experiment that require successive injection of fluids: from organic synthesis, flow chemistry, drug screening, biochemical and electrochemical sensor calibration, calibration (bench testing), sensors test rig, toxicity test, Seq-Fish experiment… Application notes How to control the flow while flow line changing with a Mux Distributor ? How to perform fast drug or medium switch? How to perform controlled drug switches with an Elveflow® MUX? How to perform an ultrafast microfluidic medium switch with an Elveflow® pressure & flow control instrument (OB1) How to perform the injection of a controlled volume of sample? For more biology-related content, please refer to the cell perfusion application pack. Reviews Microfluidics as a tool for drug delivery Electrochemistry and Microfluidics : A short review Microreactors & Microfluidics in Chemistry: a Review Microfluidic Nanoparticle Synthesis: A short review Publications Adaptable pulsatile flow generated from stem cell-derived cardiomyocytes using quantitative imaging-based signal transduction T. Qian et al., Lab on a Chip, 2020, 20, 3744-3756, DOI: 10.1039/D0LC00546K Microfluidic platform for 3D cell culture with live imaging and clone retrieval C. Mulas et al., Lab on a chip, 2020, 20, 2580-2591, DOI: 10.1039/D0LC00165A ) [specifications] => Array ( [texte] => Standard 10/1 valve NEW 12/1 valve Category Parameter Value Performances Port to port switching time (ms) 280 ms 156 ms Max recommended pressure 9 bar (125 PSI) 7 bar Internal diameter 0.5 mm 0.5 mm Internal volume(1) 11.6 µL 3.5 µL Carryover volume(2) 11.6 µL 1.7 µL Dead volume (3) None None Wetted materials PCTFE, PTFE PCTFE, PTFE Number or ports 11 13 Number of positions 10 12 Operating temperature 5-40°C 5-40°C Operating Humidity 20-70% noncondensing 20-70% noncondensing [image] => ) [download] => Array ( [fichiers] => ) [faq] => Array ( [items] => ) [more] => Array ( [more_title] => [texte] => [image] => [video] => ) [outline1] => Array ( [text] => [image] => ) [customization] => Array ( [title] => [] => [image] => [texte_1] => [texte_2] => [texte_3] => ) [outline2] => Array ( [image] => [text] => ) [produits_associes] => Array ( [0] => 13079 [1] => 324 [2] => 32021 ) [application_packs_on] => [software_on] => [distributors_on] => [services_on] => [citation_author_repeater] => [citation_publication_date] => [citation_keywords_repeater] => [description_GS] => [announcement_box] => [testimonials_prod] => [oem_product_banner] => Array ( [product_banner] => [product_title] => [product_description] => [button_label] => [button_url] => ) [ID] => 31032 [title] => Distribution Valve V2 [permalink] => https://www.elveflow.com/microfluidic-products/microfluidics-flow-measurement-sensors/distribution-valve-v2/ [post_type] => measurement [post_type_name] => Flow measurement ) ) )
Pick one of the two versions: Standard 10/1 Valve or the New 12/1 Valve.
Perfect microfluidic bidirectional selector valve for handling several samples & automating experiments!
Perform microfluidic sequential injection with the Standard 10/1 Valve or New 12/1 Valve versions of our versatile valve in combination with the OB1 MK3+ pressure controller to automate injection in microfluidic experiments, program perfusion experiments, sequentially supply different reagents and many more applications.
Use the MUX Distrib to sequentially inject up 12 different solutions into one microfluidic line for sample collections or parallel experiments.
Key advantages:
The MUX Distribution 10-1 or MUX Distributor 12-1 can be used in various fields of application and for any experiment that require successive injection of fluids: from organic synthesis, flow chemistry, drug screening, biochemical and electrochemical sensor calibration, calibration (bench testing), sensors test rig, toxicity test, Seq-Fish experiment…
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It was designed for basic control, complex workflow and automation setups. Unlimited creativity for your experiments – Thanks to the versatility and modularity of the ESI Software, any application can be easily performed and automated: droplet generation, medium perfusion and medium switch for cell and tissue culture, multiple liquid handling, nanoparticle formulation, complex sequential fluid injection, and many more. 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=> Array ( [texte] => One software for all instruments The ESI can control up to 16 Instruments (OB1 pressure controller, flow rate and pressure sensors, sets of valves and valves controller) via the same interface. Thanks to the TTL triggers, you can synchronize your Elveflow® system with any other instrument in your laboratory (from microscopes to any electrical instrument, and more). Intuitive interface The intuitive interface of the ESI microfluidic software facilitates navigation from one instrument to another. Start from simple setups and add instruments (valves, sensors, etc) through the same interface. Even the most complicated setups can be controlled in just a few clicks! A Play/Pause button allows changing pressure and flow channels, maintaining the current state of the controller while simultaneously adapting the system to the new settings. Workflow automation Built-in scheduler The scheduler is a user-friendly tool that automates complex steps of your experiments and protocols. Gain precious experimental time by: creating or editing instrument configurations (pressure or flow rate profiles, valves positions, etc) “dragging and dropping” each step to sequentially arrange them controlling steps timing building a complex sequence with loops (for) and conditions (if) integrating your setup to a wider experimental environment using trigger signals (IN and/or OUT) saving important data using the play/pause button to create a file and record the progress of your experiment saving and loading entire experiments. Advanced Software Development Kit (SDK) control Our package allows developers to integrate Elveflow systems into their own control programs. Those libraries enable fast & easy integration of our instruments to your lab project. We provide the following libraries free of charge (with documentation and example codes): C++ library, Python library, MATLAB library, LabVIEW library Discover the ESI in video Thanks to this video, you will learn how to use pressure-driven flow control with the Elveflow Software! By coupling our pressure controller with one of our flow sensors, you can perform ultra precise and responsive pressure-driven flow control. You can request a flow rate value in the Elveflow Software and the pressure controller will automatically adjust pressure to reach the requested value thanks to a customizable PID Feedback loop. [image] => ) [applications] => Array ( [texte] => ) [specifications] => Array ( [texte] => Key features Independent pressure control of each OB1 regulator channel (or flow control when connected to a flow sensor). Easy addition and control of Elveflow valves, sensors, and accessories. Real time intuitive Graph functionalities with customizable windows. Create configurations to save and recall instrument settings and easily switch from one instrument state to another (works with keyboard shortcuts). Record any data (.txt files) in a user-defined time interval up to 100Hz. Data are saved continuously to optimize computer active memory for long run experiments. Visualize your data by generating real-time graph display (pressure, flow rate, etc). Software in English. Minimum system requirement Processor: 3.0 GHz Pentium 4 RAM: 1 GB USB 2.0 1 GB of free hard disk space OS Compatibility : Windows 10, Windows 8. [image] => ) [download] => Array ( [fichiers] => ) [faq] => Array ( [items] => Array ( [0] => Array ( [question] => Do I have to pay an extra charge to obtain the ESI? [reponse] => Absolutely not, the ESI is free of charge with unlimited access. The software comes on a USB key with any instrument and can be download on this website. ) [1] => Array ( [question] => Can I have access to the ESI's latest version? [reponse] => New versions are released on a regular basis, they propose new and improved features. Good news, they are free of charge and can be downloaded directly from this page. You can also ask our support team (customer@elveflow.com) for it. ) [2] => Array ( [question] => Can I use the ESI in Mac or Linux environment? [reponse] => Unfortunately no, the ESI is only compatible with Windows. ) [3] => Array ( [question] => Is it compulsory to use the ESI to control Elveflow instruments and sensors? [reponse] => Absolutely not, free SDK librairies and documented code examples (in C++, Labview, Matlab and Python) are also available to control Elveflow instruments and sensors from your own code or software. ) ) ) [more] => Array ( [more_title] => [texte] => [image] => [video] => ) [outline1] => Array ( [text] => Use trigger signals for advanced synchronisation The Elveflow instruments and the ESI allow in & out TTL signal management. In addition to the centralized control of the entire Elveflow range, combining the workflow automation together with trigger signals allow advanced synchronization of e.g. experiments with microscope illumination and flow control. Learn more in this this application review done in collaboration with the Biozentrum Imaging core facility at the University of Basel, and Olympus France. [image] => ) [customization] => Array ( [title] => Workflow automation: a step-by-step example [] => [image] => Array ( [ID] => 80415 [id] => 80415 [title] => elveflow smart interface automation [filename] => elveflow-smart-interface-automation.jpg [filesize] => 40980 [url] => https://www.elveflow.com/wp-content/uploads/2022/02/elveflow-smart-interface-automation.jpg [link] => https://www.elveflow.com/microfluidic-products/microfluidics-software/elveflow-software-sdk/elveflow-smart-interface-automation/ [alt] => elveflow smart interface automation [author] => 27 [description] => elveflow smart interface automation [caption] => elveflow smart interface automation [name] => elveflow-smart-interface-automation [status] => inherit [uploaded_to] => 76500 [date] => 2022-03-29 07:27:14 [modified] => 2024-08-19 14:21:51 [menu_order] => 0 [mime_type] => image/jpeg [type] => image [subtype] => jpeg [icon] => https://www.elveflow.com/wp-includes/images/media/default.png [width] => 1000 [height] => 618 [sizes] => Array ( [thumbnail] => https://www.elveflow.com/wp-content/uploads/2022/02/elveflow-smart-interface-automation.jpg [thumbnail-width] => 150 [thumbnail-height] => 93 [medium] => https://www.elveflow.com/wp-content/uploads/2022/02/elveflow-smart-interface-automation.jpg [medium-width] => 300 [medium-height] => 185 [medium_large] => https://www.elveflow.com/wp-content/uploads/2022/02/elveflow-smart-interface-automation.jpg [medium_large-width] => 768 [medium_large-height] => 475 [large] => https://www.elveflow.com/wp-content/uploads/2022/02/elveflow-smart-interface-automation.jpg [large-width] => 1000 [large-height] => 618 [1536x1536] => https://www.elveflow.com/wp-content/uploads/2022/02/elveflow-smart-interface-automation.jpg [1536x1536-width] => 1000 [1536x1536-height] => 618 [2048x2048] => https://www.elveflow.com/wp-content/uploads/2022/02/elveflow-smart-interface-automation.jpg [2048x2048-width] => 1000 [2048x2048-height] => 618 ) ) [texte_1] => 1. Setup example The OB1 flow controller with a single 0-2000 mbar channel A gas splitter to apply pressure onto 4 independant reservoirs A rotary valve allowing the injection of up to 12 different liquids (4 in this example) A downstream flow sensor to accurately measure the flow rate and power up the OB1 pressure regulator into a flow controller using a precise, responsive, and stable flow rate feedback loop control. [texte_2] => 2. User requirement I want to perform the following sequence: inject solution 1 (red) at 5µl/min during 25 minutes to provide buffer to my cells switch to medium 2 (blue) containing my molecule of interest while keeping the same flow rate for 2 minutes switch to molecule 2 (yellow) containing a second molecule of interest still under continuous 5µl/min perfusion for 2 minutes perform a final washing step using medium 4 (green) and increase flow rate to 15µl/min perform this sequence three times [texte_3] => 3. ESI scheduler ) [outline2] => Array ( [image] => [text] => ) [produits_associes] => Array ( [0] => 97261 [1] => 375 [2] => 350 ) [application_packs_on] => [software_on] => [distributors_on] => [services_on] => [citation_author_repeater] => [citation_publication_date] => [citation_keywords_repeater] => [description_GS] => [testimonials_prod] => Array ( [0] => Array ( [testimonial_] => Array ( [testimonial_text] => After an easy installation, we are now using the OB1 and flow sensors routinely and are very satisfied by both the flow stability and the software's user-friendly interface [testimonial_author] => Nelson BC Serre and Matyáš Fendrych, Cell Growth Lab, Department of Experimental Plant Biology, Charles University, Czech Republic [testimonial_product] => OB1 flow controller and flow sensors user ) ) [1] => Array ( [testimonial_] => Array ( [testimonial_text] => We use Elveflow instrument in several projects and they are great to work with. The LabVIEW implementation was also very successful. I hope more people can get to know the OB1 system [testimonial_author] => Seongjin Park, Senior Engineer, Illumina [testimonial_product] => OB1 flow controller user ) ) ) [announcement_box] => [ID] => 76500 [title] => Elveflow Software and SDK [permalink] => https://www.elveflow.com/microfluidic-products/microfluidics-software/elveflow-software-sdk/ [post_type] => software [post_type_name] => Software ) ) )
Intuitive yet allowing workflow automation – The Elveflow Smart Interface provides easy and intuitive control of our microfluidic instruments. It was designed for basic control, complex workflow and automation setups.
Unlimited creativity for your experiments – Thanks to the versatility and modularity of the ESI Software, any application can be easily performed and automated: droplet generation, medium perfusion and medium switch for cell and tissue culture, multiple liquid handling, nanoparticle formulation, complex sequential fluid injection, and many more.
The ESI can control up to 16 Instruments (OB1 pressure controller, flow rate and pressure sensors, sets of valves and valves controller) via the same interface.
Thanks to the TTL triggers, you can synchronize your Elveflow® system with any other instrument in your laboratory (from microscopes to any electrical instrument, and more).
The intuitive interface of the ESI microfluidic software facilitates navigation from one instrument to another. Start from simple setups and add instruments (valves, sensors, etc) through the same interface. Even the most complicated setups can be controlled in just a few clicks!
A Play/Pause button allows changing pressure and flow channels, maintaining the current state of the controller while simultaneously adapting the system to the new settings.
The scheduler is a user-friendly tool that automates complex steps of your experiments and protocols.
Gain precious experimental time by:
Our package allows developers to integrate Elveflow systems into their own control programs. Those libraries enable fast & easy integration of our instruments to your lab project.
We provide the following libraries free of charge (with documentation and example codes):
C++ library, Python library, MATLAB library, LabVIEW library
Thanks to this video, you will learn how to use pressure-driven flow control with the Elveflow Software!
By coupling our pressure controller with one of our flow sensors, you can perform ultra precise and responsive pressure-driven flow control.
You can request a flow rate value in the Elveflow Software and the pressure controller will automatically adjust pressure to reach the requested value thanks to a customizable PID Feedback loop.
Absolutely not, the ESI is free of charge with unlimited access. The software comes on a USB key with any instrument and can be download on this website.
New versions are released on a regular basis, they propose new and improved features.
Good news, they are free of charge and can be downloaded directly from this page. You can also ask our support team (customer@elveflow.com) for it.
Unfortunately no, the ESI is only compatible with Windows.
Absolutely not, free SDK librairies and documented code examples (in C++, Labview, Matlab and Python) are also available to control Elveflow instruments and sensors from your own code or software.
The Elveflow instruments and the ESI allow in & out TTL signal management. In addition to the centralized control of the entire Elveflow range, combining the workflow automation together with trigger signals allow advanced synchronization of e.g. experiments with microscope illumination and flow control.
Learn more in this this application review done in collaboration with the Biozentrum Imaging core facility at the University of Basel, and Olympus France.
I want to perform the following sequence: inject solution 1 (red) at 5µl/min during 25 minutes to provide buffer to my cells switch to medium 2 (blue) containing my molecule of interest while keeping the same flow rate for 2 minutes switch to molecule 2 (yellow) containing a second molecule of interest still under continuous 5µl/min perfusion for 2 minutes perform a final washing step using medium 4 (green) and increase flow rate to 15µl/min perform this sequence three times
I want to perform the following sequence:
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