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This review focuses on sodium alginate and its applications in microfluidics
Finding the right technique for particle encapsulation using micro and nanoparticles is key for a successful particle encapsulation protocol.
Cell culture consists in growing cells in an artificial environment in order to study their behavior in response to their environment[1]. Different kinds of cell cultures can be found nowadays, and some would be more suited than others depending on its properties and applications.
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Highly monodispersed alginate beads can be easily generated with a microfluidic droplet generation system.
This application note explores the basic principle of pneumatic pumps and a flow controller based on the basic principle of pneumatic pumps, known as pressure driven flow control. It also demonstrates the applications of pressure driven flow control in a range of industrial & research fields.
Elveflow developped a free online microfluidic calculator. This tool has been designed to help researchers and especially non specialists of the microfluidics field. It helps you assess key parameters to configure your microfluidic experiment.
This application note explains how pressure-driven flow control works, the advantages & disadvantages of the different technologies, and the technical choice to make to how to perform effectively your microfluidic experiment.
Elveflow's microfluidic calculator permits to calculate flow rate in microfluidics dependent upon the microfluidic system from the device, the tubing to your fluid properties.
Elveflow's developped a microfluidic resistance calculator dependent upon the microfluidic system from the flow rate, the device, the tubing to your fluid properties.
The application note describes how to convert various units of viscosity from one to another: viscosity conversion from Poise, Pa.s, Dyn.s/cm²...
The application note describes how to convert various units of shear stress and/or pressure from one to another: shear stress conversion from Pascal, atmosphere, and N/m²...!
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All you need to know to perform hydrogel particle production! 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Based on our high accuracy OB1 flow controllers, this all-in-one solution contains all the required parts for researchers to set up their own alginate beads generation system out of the box. It brings the many benefits of microfluidics, including excellent monodispersity, sphericality, reproducibility, and high throughput compared to other hydrogel particle production techniques. 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618 ) ) ) [introduction_texte] => ) [principe] => Array ( [texte] => A standard alginate beads generation pack contains one pumping channel to flow the aqueous alginate phase and another pumping channel to push the continuous oil phase through our droplet generation chip, enabling the generation of alginate droplets in oil. The droplet size will be determined by the chip channel size and the flow rate ratio of both phases. Flow rates can be measured thanks to our multiple flow rate sensors (MFS or BFS series). For the detailed experimental steps, please refer to our application note! User guide alginate beads production DOWNLOAD THE USER GUIDE To help you determine your flow rate, pressure to apply, the best tubing resistance length for your setup, wall shear stress for biology applications, cell culture, and many more… Elveflow provides its microfluidic calculator and…to make the most of our microfluidic calculator, find below a set of dedicated application notes: What is a microfluidic calculator? Viscosity conversion Shear stress calculator How to calculate the flow rate with microfluidics Microfluidic resistance calculator [image] => ) [applications] => Array ( [texte] => Application notes Liquid foam templating for scaffold generation Nanohydrogel generation Microgel generation & Encapsulation How does microfluidics alginate beads production work? Reviews Microfluidics for PLGA nanoparticles synthesis: a review Confined particles in microfluidic devices: a review Sodium Alginate and applications: a review Introduction to droplet-based microfluidics Microfluidic magnetic particle sorting and separation : a short review Microfluidics as a tool for drug delivery Microfluidic Nanoparticle Synthesis: A short review Publications Droplet microfluidics as a tool for production of bioactive calcium phosphate microparticles with controllable physicochemical properties, P. Habibovic et al., Acta Biomaterialia (2021). 3D flow-focusing microfluidic biofabrication: One-chip-fits-all hydrogel fiber architectures, Rui L. Reis et al. , Applied Materials Today (2021). Additive batch electrospinning patterning of tethered gelatin hydrogel fibres with swelling-induced fibre curling Elisabeth L. Gill, Wenyu Wang, Ruishan Liu, Yan Yan Shery Huang, Additive Manufacturing, 2020, Volume 36, 101456, DOI: 10.1016/j.addma.2020.101456 Highly Ordered Gelatin Methacryloyl Hydrogel Foams with Tunable Pore Size Friederike Dehli, Lisa Rebers, Cosima Stubenrauch, and Alexander Southan, Biomacromolecules 2019 20 (7), 2666-2674 DOI: 10.1021/acs.biomac.9b00433 ) [specifications] => Array ( [texte] => The amazing benefits of microfluidics can be applied to many droplet applications and therefore the content of the alginate pack can be adjusted to suit your specific needs. Contact us for more details. CONTENT OB1 FLOW CONTROLLER with at least two channels 0/2000 mbar 2x flow sensors MFS2 0/7 µL/min (see also the Coriolis flow sensor for the best performance) Kit starter pack Luer Lock + 1/32 tubings + 1/32 sleeves + 23G needles 2x 15 mL Falcon Microfluidic chip (hydrophobic channels) 1 x vial of droplet oil control and automation software User guide which includes a beads size diagram, allowing you to pick up the right flow rates to achieve a desired bead size Options Additional pumping channels Additional flow rate sensors Computer Microscope and camera [image] => ) [download] => Array ( [fichiers] => ) [faq] => Array ( [items] => ) [more] => Array ( [more_title] => Videos [texte] => Videos [image] => [video] => ) [outline1] => Array ( [text] => Why choosing microfluidics for alginate beads generation? Microfluidics brings reproducibility and control over particle or droplet size that is not achievable by any other technique. Continuous hydrogel beads generation with constant size and flow rates control, results in highly monodisperse particles (PDI < 5%). Moreover, microfluidics is an ideal technique for handling ultra small volumes and control what goes into each droplet or bead. Microfluidics give you flexibility to easily generate identical carrier with the same content. For more detailed protocole about alginate beads generation, please refer to our application note! [image] => ) [customization] => Array ( [title] => Configure your alginate pack [] => [image] => [texte_1] => 1. Select the right parameters to obtain the desired beads size Elveflow offers a specific chip that allows you to generate a broad of range of beads size, shape and materials. For more information, please refer to our application note about alginate beads generation. If you’re unsure about the settings that are best suited for your application, get in touch with one of our experts! [texte_2] => 2. Choose your reservoir size Are you working with small samples or are you looking into manufacturing 100x mLs of hydrogel beads solution? Elveflow offers a comprehensive range of reservoirs compatible with our OB1 flow controller, from 1.5 mL Eppendorf tubes to 100mL bottles. [texte_3] => 3. Oil & surfactants To make monodisperse and stable alginate beads, Elveflow offers a range of oils and surfactants. For more detailed information, please refer to this application note! ) [outline2] => Array ( [image] => [text] => Interested in highly monodisperse hydrogel beads production? Hydrogel-based delivery systems are finding more and more applications, from encapsulation, protection, to the release of bioactive agents in many fields such as pharmaceutical, supplements, food industries, for cell/bacteria/microbes culture & implantation, and cell-based genes in biological research. Are you interested in implementing hydrogel beads production in your lab? Contact us to have your dedicated your dedicated hydrogel beads generation pack that suits your application needs! Highly monodispersed alginate droplets, size in the 50µm range. Experiment results by the Elveflow team. ) [produits_associes] => Array ( [0] => 97261 [1] => 375 [2] => 382 [3] => 1025 ) [application_packs_on] => [software_on] => [distributors_on] => [services_on] => [citation_author_repeater] => [citation_publication_date] => [citation_keywords_repeater] => [description_GS] => [testimonials_prod] => Array ( [0] => Array ( [testimonial_] => Array ( [testimonial_text] => We are very satisfied of the ELVEFLOW pressure pumps! The ELVEFLOW pressure pumps enable us to perform portable experiments with accurate pressure control. [testimonial_author] => Dr. Wiebcke Drenckhan, Liquids Interfaces Group of the Physic of Solids Lab - CNRS UMR 8502 at Orsay University, France [testimonial_product] => OB1 pressure-driven flow controller user [testimonial_image] => [testimonial_university_country_v2] => ) ) [1] => Array ( [testimonial_] => Array ( [testimonial_text] => I appreciate rapid setup time and the fact that we can very precisely adjust the flow rates through the user-friendly interface. [testimonial_author] => Dr. Caglar Elbuken, UNAM - Bilkent University, Turkey [testimonial_product] => OB1 pressure-driven flow controller user [testimonial_image] => [testimonial_university_country_v2] => ) ) [2] => Array ( [testimonial_] => Array ( [testimonial_text] => We are satisfied with the ELVEFLOW instrument, the regulation accuracy is well fitted with our applications. [testimonial_author] => Pr. 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[2048x2048-width] => 444 [2048x2048-height] => 251 ) ) [part_1] => Droplet Pack [part_2] => Droplet generation pack [part_3] => Turnkey system to easily generate droplets and control all key parameters. 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Set up. Get droplets. ) [1] => Array ( [icon] => icon-check [titre] => Reproducible & easy generation [texte] => Produce highly monodisperse droplets (CV<3%) ) [2] => Array ( [icon] => icon-check [titre] => Perfect for many applications [texte] => Particle generation, encapsulation, emulsions... and many more! ) ) ) [introduction_texte] => An all-inclusive Pack to generate droplets out-of-the box. We setup a dedicated set of instruments, controlled by a unique software, to ease the droplet generation process. It brings the many benefits of microfluidics, such as excellent monodispersity, reproducibility and scalability to your daily work in order to achieve great science. It has never been easier to start generating droplets following a step-by-step User Guide and quickly master the process with the same system. 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We designed a comprehensive solution based on our high precision instruments set. This Pack includes all you need to understand the droplet generation process from 0 to 1.
Alginate beads production has never been that easy using our all-in-one hydrogel particle generation system!
Based on our high accuracy OB1 flow controllers, this all-in-one solution contains all the required parts for researchers to set up their own alginate beads generation system out of the box. It brings the many benefits of microfluidics, including excellent monodispersity, sphericality, reproducibility, and high throughput compared to other hydrogel particle production techniques.
A standard alginate beads generation pack contains one pumping channel to flow the aqueous alginate phase and another pumping channel to push the continuous oil phase through our droplet generation chip, enabling the generation of alginate droplets in oil.
The droplet size will be determined by the chip channel size and the flow rate ratio of both phases. Flow rates can be measured thanks to our multiple flow rate sensors (MFS or BFS series).
For the detailed experimental steps, please refer to our application note!
DOWNLOAD THE USER GUIDE
To help you determine your flow rate, pressure to apply, the best tubing resistance length for your setup, wall shear stress for biology applications, cell culture, and many more…
Elveflow provides its microfluidic calculator and…to make the most of our microfluidic calculator, find below a set of dedicated application notes:
Droplet microfluidics as a tool for production of bioactive calcium phosphate microparticles with controllable physicochemical properties, P. Habibovic et al., Acta Biomaterialia (2021).
3D flow-focusing microfluidic biofabrication: One-chip-fits-all hydrogel fiber architectures, Rui L. Reis et al. , Applied Materials Today (2021).
Additive batch electrospinning patterning of tethered gelatin hydrogel fibres with swelling-induced fibre curling
Elisabeth L. Gill, Wenyu Wang, Ruishan Liu, Yan Yan Shery Huang, Additive Manufacturing, 2020, Volume 36, 101456, DOI: 10.1016/j.addma.2020.101456
Highly Ordered Gelatin Methacryloyl Hydrogel Foams with Tunable Pore Size
The amazing benefits of microfluidics can be applied to many droplet applications and therefore the content of the alginate pack can be adjusted to suit your specific needs. Contact us for more details.
Microfluidics brings reproducibility and control over particle or droplet size that is not achievable by any other technique. Continuous hydrogel beads generation with constant size and flow rates control, results in highly monodisperse particles (PDI < 5%).
Moreover, microfluidics is an ideal technique for handling ultra small volumes and control what goes into each droplet or bead. Microfluidics give you flexibility to easily generate identical carrier with the same content. For more detailed protocole about alginate beads generation, please refer to our application note!
Elveflow offers a specific chip that allows you to generate a broad of range of beads size, shape and materials. For more information, please refer to our application note about alginate beads generation.
If you’re unsure about the settings that are best suited for your application, get in touch with one of our experts!
Are you working with small samples or are you looking into manufacturing 100x mLs of hydrogel beads solution? Elveflow offers a comprehensive range of reservoirs compatible with our OB1 flow controller, from 1.5 mL Eppendorf tubes to 100mL bottles.
To make monodisperse and stable alginate beads, Elveflow offers a range of oils and surfactants. For more detailed information, please refer to this application note!
Hydrogel-based delivery systems are finding more and more applications, from encapsulation, protection, to the release of bioactive agents in many fields such as pharmaceutical, supplements, food industries, for cell/bacteria/microbes culture & implantation, and cell-based genes in biological research.
Are you interested in implementing hydrogel beads production in your lab?
Contact us to have your dedicated your dedicated hydrogel beads generation pack that suits your application needs!
Highly monodispersed alginate droplets, size in the 50µm range. Experiment results by the Elveflow team.
An all-inclusive Pack to generate droplets out-of-the box. We setup a dedicated set of instruments, controlled by a unique software, to ease the droplet generation process.
It brings the many benefits of microfluidics, such as excellent monodispersity, reproducibility and scalability to your daily work in order to achieve great science.
It has never been easier to start generating droplets following a step-by-step User Guide and quickly master the process with the same system.
Do you want to implement droplet-based microfluidics? We designed a comprehensive solution based on our high precision instruments set. This Pack includes all you need to understand the droplet generation process from 0 to 1.
Start from scratch and quickly master all key parameters triggering and controlling droplet generation.
Droplet forming inside the microfluidic chip
Microfluidics brings reproducibility and control over droplet size that are not achievable with any other technique.
Moreover, it is an ideal technique to handle ultra small volumes and control droplet generation precisely. It give you flexibility to easily generate identical droplets with the same content or to create individual droplets with unique payloads.
This Droplet Generation Pack includes our OB1 flow controller best-seller.
Thanks to the performance of this instrument, you will be able to generate:
We can provide you with two Droplet Pack versions : the small version, to generate smaller droplets ranging from 10 to 40µm and the large version, for droplets ranging from 50 to 80µm diameter. Each Pack is composed of:
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All our application notes
All our reviews
And more!
Our two versions only differ by the flow rate range of the oil phase.
Important note: our products are all intercompatible and you may acquire additional flow rate sensors or add extra products for specific applications. You will still benefit from the centralised ESI software control.
Droplet-based webinars
Droplet-based applications
Elveflow developped a microfluidic calculator that allows you to estimate all the key parameters at stake in your microfluidic system. Find out more below !
The microfluidic chip is where the two immiscible phases (water and oil here) are precisely injected and mixed to generate monodisperse droplets (CV < 3%). The two microfluidic chips are made of Topas. Topas is a cyclic olefin copolymer (COC) resin which is a chemical relative of polyethylene and other polyolefin plastics.
Are you working with small samples or are you looking into manufacturing 100x mLs of emulsion? Elveflow offers a comprehensive range of reservoirs compatible with our OB1 Flow Controller, from 1.5 mL Eppendorf tubes to 100mL bottles.
Flow resistances are used to increase the resistivity of the microfluidic system to improve the stability and control of the flow rate in the system.
Simple and intuitive instructions are provided to quickly and easily make droplets and control droplet generation parameters. When starting out, the user can follow step by step the provided protocol to obtain droplets of the specific size. In a second stage, the user can rely on the numerous tips provided and explore the “going further” section to complete its training in droplet generation and microfluidic flow control.
Everything you need to start running microfluidics experiments right away.
Elveflow’s Starter Pack contains all necessary elements for you to start your own microfluidic experiments. Based on Elveflow’s popular OB1 Flow Controller, this easy-to-use system covers the majority of microfluidics researchers’ needs. It is fully compatible with the whole Elveflow product range, enabling you to upgrade your system as your needs grow.
From simple single-channel microfluidic flow to multi-channel droplet experiments, the Starter Pack covers hardware needs for a wide range of applications. The whole system is controlled by the powerful ESI software which allows you to simply set pressures and monitor your experiment, or try advance functions such as full automation and running scripts.
You can add different Elveflow modules to suit your experimental needs. These modules include valves, sensors, bubble traps and many more.
Experiments performed with the microfluidics Starter Pack
Microfluidics can be applied to lots of varied applications and therefore the content of the Starter Pack can be tweaked to suit your specific needs.
A standard starter pack contains the following:
Microfluidics is the science of manipulating and controlling fluids, usually in the range of microliters (10-6) to picoliters (10-12). It is an amazing technology that brings lots of benefits to many varied applications areas of science such as:
Pressure-driven flow controlled microfluidics: Switch from an accurate pressure control to an effective flow rate control by adding Elveflow’s Flow Rate Sensors.
Those sensors, combined with the ESI software and its built-in feedback loop control, enable monitoring and control of liquid flow rates. You can dial in a flow rate value and your system will quickly and accurately reach that flow rate by adjusting the system pressure.
Elveflow offers a variety of chips designed for specific applications such as droplet generation, flow focusing, cell encapsulation, and many more.
The OB1 Flow Controller is a powerful instrument that can be tailored to suit your needs. We offer different channels, for vacuum and/or pressure, from -900 mBar up to 8000 mBar. You can swap or add channels to your OB1 Flow Controller at a later date. Contact us to find out more about the procedure.
Organoids and spheroids are three-dimensional structures composed of self-assembled cells that mimic the characteristics of tissues and organs. These structures are typically grown by culturing cells under non-adherent conditions that allow them to self-organize into these complex structures [1]. A current challenge is to integrate cellular complexity into organoids in a controlled manner, leading to organized assembly and takeover of tissue function [2].
Using microfluidics for organoids or spheroids in droplets:
Elveflow offers you a versatile computer-controlled microfluidic setup to enable organoid & spheroid encapsulation in an efficient manner.
One technique for creating organoids and spheroids is to encapsulate cells in droplets of a hydrogel, such as alginate or agarose, using microfluidic devices. The droplets act as small bioreactors, providing the cells with the necessary nutrients and oxygen for growth, while also allowing for the exchange of waste products.
This pack is composed of the premium Elveflow product line and our best-seller, the OB1 flow controller. Thanks to the high performance of this equipment set, you will be able to:
The solution can be configured as per the user’s requirements. Our experts can help you define the best possible setup to reach your goal.
Thanks to the versatility of the instruments, and the upgradability of our range, Elveflow’s solution can be adapted to your needs.
As proof of concept, you can consult this Nature paper on Tumour-on-chip microfluidic platform for assessment of drug pharmacokinetics and treatment response
References
[1] Kratochvil, M. J., Seymour, A. J., Li, T. L., Paşca, S. P., Kuo, C. J., & Heilshorn, S. C. (2019). Engineered materials for organoid systems. Nature Reviews Materials, 4(9), 606-622.
[2] Takebe, T., & Wells, J. M. (2019). Organoids by design. Science, 364(6444), 956-959.
Beta Packs, such as the Dielectrophoretic Sorting Pack pack, are an assembly of instruments that include Elveflow’s catalog products and, at least, one beta instrument. The beta instrument has recently been developed by our Innovation team, and it is in testing phase. If you are interested in this beta pack, its applications, or possible variations to better fit your needs, contact our experts!
Our Beta Packs, such as the Dielectrophoretic sorting are available under specific conditions. As these packs are still in the developmental stage, we have some eligibility criteria to ensure their optimal success rate. A consultation with our experts is necessary to evaluate your eligibility to our Beta Testing Program.
At Elveflow, we believe in tailoring our approach to suit each researcher’s unique needs. Therefore, before purchasing a Beta Pack, we encourage you to reach out to our technical team for a detailed discussion of your research objectives and requirements.
To learn more about our Beta Packs or to schedule a consultation with our experts, please contact us at innovation@elvesys.com. We look forward to collaborating with you and supporting your research endeavors.
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Explore how phase separation, dynamic compartmentalization, and lab-on-a-chip techniques redefine synthetic cell engineering. 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Array ( [image] => Array ( [ID] => 3563 [id] => 3563 [title] => Fig 3 Chiara Martino microgel generation encapsulation e1578930851922 [filename] => Fig-3-Chiara-Martino-microgel-generation-encapsulation-e1578930851922.png [filesize] => 17362 [url] => https://elveflow.com/wp-content/uploads/2020/01/Fig-3-Chiara-Martino-microgel-generation-encapsulation-e1578930851922.png [link] => https://elveflow.com/microfluidics-research-summaries/microgel-generation-encapsulation-microdroplets/fig-3-chiara-martino-microgel-generation-encapsulation/ [alt] => Fig 3 Chiara Martino microgel generation encapsulation e1578930851922 [author] => 27 [description] => Fig 3 Chiara Martino microgel generation encapsulation e1578930851922 [caption] => Fig 3 Chiara Martino microgel generation encapsulation e1578930851922 [name] => fig-3-chiara-martino-microgel-generation-encapsulation [status] => inherit [uploaded_to] => 3556 [date] => 2020-01-13 15:53:49 [modified] => 2024-08-19 14:21:27 [menu_order] => 0 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A continuous-flow photolithography was set up within a cross junction to generate and simultaneously to in-situ encapsulate fiber-like structures in pico-droplets. ) [insert] => ob1fc2 [utiliser_acf] => [most_popular] => [review_type] => 11 [citation_author_repeater] => Array ( [0] => Array ( [citation_author] => Elveflow Team ) ) [citation_publication_date] => 2021/02/05 [citation_keywords_repeater] => Array ( [0] => Array ( [citation_keywords] => droplet based microfluidics ) ) [description_GS] => PEGDA (poly (ethylene glycol) diacrylate) microgels were generated and encapsulated within pico-droplets by combining droplet-based microfluidics and microscope projection photolithography. A continuous-flow photolithography was set up within a cross junction to generate and simultaneously to in-situ encapsulate fiber-like structures in pico-droplets. 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Discover how a microfluidic constriction assay in large cardiac spheroids replicates myocardial ischemia and fibrotic remodeling to advance cardiovascular research.
The authors developed, tested and validated a method that applies a pressure-driven flow controller to fabricate biocompatible micron-scale silk fibers by microfluidic wet spinning.
PEGDA (poly (ethylene glycol) diacrylate) microgels were generated and encapsulated within pico-droplets by combining droplet-based microfluidics and microscope projection photolithography. A continuous-flow photolithography was set up within a cross junction to generate and simultaneously to in-situ encapsulate fiber-like structures in pico-droplets.
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Thanks to our proprietary Piezoelectronic Technology, the OB1 MK3+ is 10 times more stable and up to 10 times faster than other microfluidic flow controllers. 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perfect flow control for all kinds of applications. Whether you need pressure or vacuum, low or high flow rate, for short or week-long processes and experiments, the OB1 MK4 is the ideal instrument for your microfluidic needs. Check out our OB1 animation here! The best performance on the market: Piezoelectric Technology The Elveflow OB1 MK4 is one of the best performing microfluidic flow control instruments worldwide that uses piezoelectric regulators. The piezoelectric technology gives you 20 times more precise and 10 times faster flow control than any other flow controller on the market. Customizable & upgradable: 1 module, up to 4 channels, 5 pressure & vacuum ranges available The OB1 MK4 can be configured according to your needs. In one piece of equipment, you can have up to 4 pressure and/or vacuum channels (and other customized options). If your needs change, the instrument can be upgraded later, in any way you want. Get the fastest flow rate control when paired with a flow sensor Connect the OB1 MK4 to a standard liquid flow rate sensor (MFS) or our premium Coriolis flow sensor (BFS, suitable for both liquid and gas) to directly control the flow rate in your chip. The system continuously calculates the pressure and maintains the desired and constant flow rate. Full control software, SDK, and UART communication Single and intuitive software to get started in a few clicks and automate complex and long experiments. The SDK libraries allow you to control the OB1 MK4 using your own code while connecting it to other instruments. The MK4 is also equipped with UART communication protocol in addition to the ESI and SDKs control, allowing it to communicate with most control systems, such as Mac, Linux, Arduino, PLC. OEM version available The OB1 MK4 can be used on a bench setup or embedded in your own product. Elveflow has a solution for every step of your research & development. Discover our OB1 MK4 OEM solution. [image] => ) [applications] => Array ( [texte] => Applications of Pressure-Driven Flow Control Thanks to its ultra-fast response time, high stability, and precise flow control, the OB1 pressure controller is a preferred choice for a wide range of liquid handling microfluidic applications, compared to syringe pumps or peristaltic pumps. Combined with precision flow sensors (like MFS or BFS), the OB1 pressure controller can accurately control flow rate in any microfluidic setups. Below is an overview of how pressure-driven flow control improves research across different domains. Droplet & Bead Generation Droplet Microfluidics Flow control microfluidics offers unmatched stability and responsiveness when generating monodisperse droplets in microchannels. The precise flow control is essential for the generation of identical droplets avoiding most post-synthesis steps. This monodispersity is essential in applications like digital PCR, single-cell analysis, or encapsulation. Explore droplet generation setup Alginate Bead Generation In biomedical research, alginate beads are used to encapsulate cells or molecules. Pressure-driven systems allow fine-tuned control over flow rates, ensuring uniform bead size and reliable encapsulation efficiency. See the alginate bead pack. Biology & Cell Culture Cell Perfusion Pressure-based perfusion ensures bubble-free, pulsation-free delivery of nutrients or drugs to cultured cells over extended periods, ideal for mimicking physiological flow conditions. Discover perfusion setup Organ-on-a-Chip Simulating organ-level responses on chips requires controlled fluid environments. Pressure-driven systems provide the accuracy and reactivity needed to replicate dynamic flow patterns and shear stress. Learn about Organ-on-a-Chip systems Cell Confinement Assays Experiments that study how cells adapt to physical constraints benefit from stable pressure control to reproducibly apply mechanical stress. See confinement assay example Cell Constriction Assays Revealing cellular responses to fibrotic environments or disease models requires reproducible deformation of cells, enabled by finely tuned pressure-controlled flows. View ischemia model example Cell Trapping & Synthetic Compartments Pressure-driven flows help trap and localize cells or materials in adapted microfluidic chips, useful in synthetic biology or compartmentalization studies. Read the synthetic cells study Flow Control & Mixin Sequential Injection & Mixing Delivering fluids in a precise sequence or generating on-chip gradients requires fast and programmable flow transitions. Pressure controllers enable seamless transitions between multiple fluid inlets. Explore the injection pack Recirculation Some in vitro assays require long term dynamic flow of reagents or cell culture media. In most cases it is essential to reuse the media, in order to mimic physiological conditions or avoid expenses. Using pressure-based flow control, one-way recirculation is possible to program for long term experiments.Learn about the recirculation setup Analytical Chemistry & Imaging Flow Chemistry Pressure-driven systems provide accurate reagent dosing and fast startup/shutdown, essential for reaction optimization in continuous-flow chemistry. This is particularly useful in the development of immunoassays and biosensors. See flow chemistry application Liquid-Phase Electron Microscopy (LPEM) Stable, pulseless flow is critical for imaging samples in real time at the nanoscale. Pressure control allows precise delivery of fluids during live-cell imaging under an electron beam, especially to study samples in their natural environment. Explore LPEM research Light-Field Flow Cytometry (LFC) In high-throughput single-cell analysis, stable and controlled flow conditions are key to accurate imaging and data capture. Pressure-based systems enable high-speed and consistent sample handling. Learn about 3D LFC Industrial Applications Enhanced Oil Recovery (EOR) Microfluidic chips simulating porous media allow researchers to visualize oil displacement mechanisms. Pressure control enables fine adjustment of flow to mimic subsurface conditions with high reproducibility. Read the full EOR review Cosmetic Formulation & Testing Microfluidics offers new possibilities for precision formulation of cosmetic emulsions and nano-encapsulation of active ingredients. Pressure-driven flow ensures reproducibility and scalability for testing stability, texture, or skin permeability on-chip. Explore cosmetic applications Integrated Systems Lab-on-a-Chip Platforms At the core of any lab-on-a-chip system is the need for precise, responsive, and stable flow control, features inherently supported by pressure-driven microfluidics. Whether it’s diagnostics, environmental sensing, or point-of-care testing, the OB1 system provides the control backbone required for success. Discover some lab-on-chip examples in this review Videos Publications For all publications featuring our products, please click here. For all application notes based on our product, please click here. For reviews of our product, please click here. ) [specifications] => Array ( [texte] => This table summarizes the main specifications of the Elveflow OB1 MK4 pressure controller. OB1 MK4 CHANNEL PRESSURE RANGE 0 to 200 mbar1 (0 to 2.9 psi) 0 to 2,000 mbar1 (0 to 29 psi) 0 to 8,000 mbar1 (0 to 116 psi) -900 to 1,000 mbar1 (-13 to 14.5 psi) -900 to 6,000 mbar1 (-13 to 87 psi) Pressure stability (2) 0.015% FS 30 µbar (0.0004 psi) 0.005% FS 100 µbar (0.0014 psi) 0.006% FS 500 µbar (0.007 psi) -900 to 500 mbar: 0.005% FS 100 µbar (0.0014 psi) 500 to 1,000 mbar: 0.007% FS 150 µbar (0.0021 psi) -900 to 2,000 mbar: 0.005% FS 350 µbar (0.005 psi) 2,000 to 6,000 mbar: 0.007% FS 525 µbar (0.008 psi) Response time (3) down to 10 ms Settling time (4) down to 50 ms Minimum pressure increment 0.006% FS 12 µbar – 0.00017 psi 0.006% FS 120 µbar – 0.0017 psi 0.006% FS 480 µbar – 0.007 psi 0.0064% FS 120 µbar – 0.0017 psi 0.0061% FS 420 µbar – 0.006 psi Pressure supply 1.5 bar to 10 bar Non corrosive, non explosive, dry and oil-free gases, e.g., air, argon, N2, CO2, … Input vacuum (5) / / / Any value from -0.7 to -1 bar Compatible with vacuum pump or vacuum line Any value from -0.7 to -1 bar Compatible with vacuum pump or vacuum line Liquid compatibility Non contact pump Any aqueous, oil, or biological sample solution. Non-contractual information, may be changed without notice (1) Max pressure value might vary by +/- 2.5% (2)Pressure stability (standard deviation) measured over the full pressure range with an external high accuracy pressure sensor (Druck DPI150) (3) Time required to reach 5% of the setting point. Depends on the computer operating system (4) Time required to reach 95% of the set point. Volume dependent – Measurement was done on 12 mL reservoir for a set point from 0 to 200 mbar (5) A vacuum source is mandatory for calibration and use of dual channels even if the channels are to be used in pressure only Flow control Flow sensor compatibility Compatible with the whole MFS and BFS range Monitoring and feedback loop flow control available Flow rates From 0,1 µL/min to 500 mL/min (indicative, please refer to the MFS and BFS series) Liquid compatibility Non-contact pump Any aqueous, oil, or biological sample solution. Control & monitoring Software control Elveflow Smart Interface – Windows 7, 8, 10, both 32 and 64 bit versions supported Software Development Kit Libraries available: Matlab, Python, LabView, C++ – Windows 7 & 10, both 32 & 64 bit versions supported Serial/UART communication protocol on request Data management Possibility to log and extract data (CSV): channel and sensor detailed information using ESI Input profiles Possibility to load profiles: ramp, sine, triangle, square, or custom Automation Generate step-by-step sequences using the ESI built-in sequence management Log and export custom configurations (CSV) Screen LCD screen showing pressure and sensor flow rate in real time Electrical Specifications Input Voltage (V) 24V Typical Power (W) 12W Provided Power Supply Specifications Supply Voltage Range (V): 100 to 240 VAC Supply AC Frequency (Hz): 50 to 60 Hz Maximum Output Current (A): 1.5 A Maximum Output Power (W): 36W Interface USB Type B Communication Type Serial Software Control ESI Sensor Connection One M8 4-pins connector per channel Compatibility Elveflow sensors: MFS, MPS, MFP, MBD Custom sensors: 5 to 24V supply voltage, 0 to 10V readout voltage Triggers Input and Output TTL signal 0V or 5V Other Casing dimensions (length x width x height): 240 x 223 x 80 mm Weight 1.4 kg to 2.9 kg Non-contractual information, may be changed without notice [image] => ) [download] => Array ( [fichiers] => ) [faq] => Array ( [items] => Array ( [0] => Array ( [question] => What pressure range should I choose? [reponse] => The performances of the OB1 (for example, the pressure stability) is tied to the full operating pressure range. In order to achieve optimum performance, we recommend our users to choose the smallest pressure range that covers the required experimental pressures. ) [1] => Array ( [question] => Can I achieve direct flow control? [reponse] => By coupling a MFS or BFS flow sensor to the OB1 flow controller, you can use the software to directly control the flow rate by directly inputting the flow rate value. The software uses a PID loop to control the flow rate by setting automatically the pressure to reach the requested flow rate. Doing so, you can benefit of the best of the two worlds to get a fast and precise (pressure driven) volume flow rate (flow sensor). ) [2] => Array ( [question] => Can I connect a Pressure Sensor to the OB1? - [reponse] => Pressure and flow sensors can be connected directly to the OB1 MK4. The sensors can be used in passive mode (for monitoring only) or active mode (to enslave). ) [3] => Array ( [question] => Do I need a pressure and/or vacuum source to operate the OB1 Pressure controller ? [reponse] => Yes, you will need a pressure source to work with the OB1 pressure controller. You can either use the air supply of your lab if there is one, use a compressed air bottle or use an air compressor. Elveflow has selected a compressor that is perfectly fitted for most application using the OB1. If your OB1 has a vacuum channel, you will need a vacuum pump. Elveflow also proposes a selected pump that work perfectly with the OB1. ) [4] => Array ( [question] => How to install the OB1? [reponse] => Click here Unboxing video ) ) ) [more] => Array ( [more_title] => Software [texte] => Software ESI is Elveflow’s dedicated software interface, built to make life easier for experimenters. It is perfectly adapted for the control of simple and complex setups and workflow automation. It integrates several modules that make time-consuming and painful tasks simple. It comes with SDK libraries to control the Elveflow system from your own code. The OB1 control window allows to:
The OB1 allows you to control the output pressure of up to 4 channels independently, from -900 mbar to 8 bar, for a wide variety of advanced microfluidic applications.
Thanks to our proprietary Piezoelectronic Technology, the OB1 MK3+ is 10 times more stable and up to 10 times faster than other microfluidic flow controllers.
Designed by scientists for scientists, the versatile and powerful OB1 MK4 pressure controller provides the perfect flow control for all kinds of applications. Whether you need pressure or vacuum, low or high flow rate, for short or week-long processes and experiments, the OB1 MK4 is the ideal instrument for your microfluidic needs.
Check out our OB1 animation here!
The Elveflow OB1 MK4 is one of the best performing microfluidic flow control instruments worldwide that uses piezoelectric regulators. The piezoelectric technology gives you 20 times more precise and 10 times faster flow control than any other flow controller on the market.
The OB1 MK4 can be configured according to your needs. In one piece of equipment, you can have up to 4 pressure and/or vacuum channels (and other customized options). If your needs change, the instrument can be upgraded later, in any way you want.
Connect the OB1 MK4 to a standard liquid flow rate sensor (MFS) or our premium Coriolis flow sensor (BFS, suitable for both liquid and gas) to directly control the flow rate in your chip. The system continuously calculates the pressure and maintains the desired and constant flow rate.
Single and intuitive software to get started in a few clicks and automate complex and long experiments. The SDK libraries allow you to control the OB1 MK4 using your own code while connecting it to other instruments. The MK4 is also equipped with UART communication protocol in addition to the ESI and SDKs control, allowing it to communicate with most control systems, such as Mac, Linux, Arduino, PLC.
The OB1 MK4 can be used on a bench setup or embedded in your own product. Elveflow has a solution for every step of your research & development. Discover our OB1 MK4 OEM solution.
Thanks to its ultra-fast response time, high stability, and precise flow control, the OB1 pressure controller is a preferred choice for a wide range of liquid handling microfluidic applications, compared to syringe pumps or peristaltic pumps. Combined with precision flow sensors (like MFS or BFS), the OB1 pressure controller can accurately control flow rate in any microfluidic setups. Below is an overview of how pressure-driven flow control improves research across different domains.
Droplet Microfluidics Flow control microfluidics offers unmatched stability and responsiveness when generating monodisperse droplets in microchannels. The precise flow control is essential for the generation of identical droplets avoiding most post-synthesis steps. This monodispersity is essential in applications like digital PCR, single-cell analysis, or encapsulation. Explore droplet generation setup
Alginate Bead Generation In biomedical research, alginate beads are used to encapsulate cells or molecules. Pressure-driven systems allow fine-tuned control over flow rates, ensuring uniform bead size and reliable encapsulation efficiency. See the alginate bead pack.
Cell Perfusion Pressure-based perfusion ensures bubble-free, pulsation-free delivery of nutrients or drugs to cultured cells over extended periods, ideal for mimicking physiological flow conditions. Discover perfusion setup
Organ-on-a-Chip Simulating organ-level responses on chips requires controlled fluid environments. Pressure-driven systems provide the accuracy and reactivity needed to replicate dynamic flow patterns and shear stress. Learn about Organ-on-a-Chip systems
Cell Confinement Assays Experiments that study how cells adapt to physical constraints benefit from stable pressure control to reproducibly apply mechanical stress. See confinement assay example
Cell Constriction Assays Revealing cellular responses to fibrotic environments or disease models requires reproducible deformation of cells, enabled by finely tuned pressure-controlled flows. View ischemia model example
Cell Trapping & Synthetic Compartments Pressure-driven flows help trap and localize cells or materials in adapted microfluidic chips, useful in synthetic biology or compartmentalization studies. Read the synthetic cells study
Sequential Injection & Mixing Delivering fluids in a precise sequence or generating on-chip gradients requires fast and programmable flow transitions. Pressure controllers enable seamless transitions between multiple fluid inlets. Explore the injection pack
Recirculation Some in vitro assays require long term dynamic flow of reagents or cell culture media. In most cases it is essential to reuse the media, in order to mimic physiological conditions or avoid expenses. Using pressure-based flow control, one-way recirculation is possible to program for long term experiments.Learn about the recirculation setup
Flow Chemistry Pressure-driven systems provide accurate reagent dosing and fast startup/shutdown, essential for reaction optimization in continuous-flow chemistry. This is particularly useful in the development of immunoassays and biosensors. See flow chemistry application
Liquid-Phase Electron Microscopy (LPEM) Stable, pulseless flow is critical for imaging samples in real time at the nanoscale. Pressure control allows precise delivery of fluids during live-cell imaging under an electron beam, especially to study samples in their natural environment. Explore LPEM research
Light-Field Flow Cytometry (LFC) In high-throughput single-cell analysis, stable and controlled flow conditions are key to accurate imaging and data capture. Pressure-based systems enable high-speed and consistent sample handling. Learn about 3D LFC
Enhanced Oil Recovery (EOR) Microfluidic chips simulating porous media allow researchers to visualize oil displacement mechanisms. Pressure control enables fine adjustment of flow to mimic subsurface conditions with high reproducibility. Read the full EOR review
Cosmetic Formulation & Testing
Microfluidics offers new possibilities for precision formulation of cosmetic emulsions and nano-encapsulation of active ingredients. Pressure-driven flow ensures reproducibility and scalability for testing stability, texture, or skin permeability on-chip. Explore cosmetic applications
Lab-on-a-Chip Platforms At the core of any lab-on-a-chip system is the need for precise, responsive, and stable flow control, features inherently supported by pressure-driven microfluidics. Whether it’s diagnostics, environmental sensing, or point-of-care testing, the OB1 system provides the control backbone required for success. Discover some lab-on-chip examples in this review
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This table summarizes the main specifications of the Elveflow OB1 MK4 pressure controller.
Non-contractual information, may be changed without notice
(1) Max pressure value might vary by +/- 2.5% (2)Pressure stability (standard deviation) measured over the full pressure range with an external high accuracy pressure sensor (Druck DPI150) (3) Time required to reach 5% of the setting point. Depends on the computer operating system (4) Time required to reach 95% of the set point. Volume dependent – Measurement was done on 12 mL reservoir for a set point from 0 to 200 mbar (5) A vacuum source is mandatory for calibration and use of dual channels even if the channels are to be used in pressure only
The performances of the OB1 (for example, the pressure stability) is tied to the full operating pressure range.
In order to achieve optimum performance, we recommend our users to choose the smallest pressure range that covers the required experimental pressures.
By coupling a MFS or BFS flow sensor to the OB1 flow controller, you can use the software to directly control the flow rate by directly inputting the flow rate value.
The software uses a PID loop to control the flow rate by setting automatically the pressure to reach the requested flow rate. Doing so, you can benefit of the best of the two worlds to get a fast and precise (pressure driven) volume flow rate (flow sensor).
Pressure and flow sensors can be connected directly to the OB1 MK4. The sensors can be used in passive mode (for monitoring only) or active mode (to enslave).
Yes, you will need a pressure source to work with the OB1 pressure controller.
You can either use the air supply of your lab if there is one, use a compressed air bottle or use an air compressor. Elveflow has selected a compressor that is perfectly fitted for most application using the OB1.
If your OB1 has a vacuum channel, you will need a vacuum pump. Elveflow also proposes a selected pump that work perfectly with the OB1.
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Unboxing video
ESI is Elveflow’s dedicated software interface, built to make life easier for experimenters. It is perfectly adapted for the control of simple and complex setups and workflow automation. It integrates several modules that make time-consuming and painful tasks simple. It comes with SDK libraries to control the Elveflow system from your own code.
The OB1 control window allows to:
For more details about Pressure driven flow control, please read this application note.
By connecting a MFS or BFS Flow Sensor to the OB1 MK4, you enable direct rapid and precise regulation of the flow rate. The PID algorithm of the software reads the flow rate and adjusts the pressure accordingly to reach the target flow rate with speed and precision.
We have several pressure channel options available covering a wide range, up to 8000 mbar (116 PSI) and down to -900 mbar (-13 PSI). Choose the pressure channel that covers your experimental pressure requirements.
The OB1 MK4 is a compact module that can accommodate up to 4 channels (pressure and/or vacuum). Each module can be upgraded at any time and have channels added or changed. You can control as many channels as you want with one computer.
Need more channels on one module (8, 16, 64…. or even more)? Our custom service would design a specific pressure controller that fits your needs.
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