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[introduction_texte] => Image Credit: J. Adam Fenster / University of Rochester. Licence: Attribution 4.0 International (CC BY 4.0)
Image Credit: Norovirus – colour-enhanced. David Gregory & Debbie Marshall. Licence: Attribution 4.0 International (CC BY 4.0)
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[texte] => Biosensor testing
what is it?
This beta pack is dedicated to the test, characterization, and calibration of biosensors with small volumes, reproducibility, and automatization . We have sought to create a system that unifies and automates the tests necessary to calibrate any biosensor based on Elveflow instruments .
The main objective of this pack is to create a scalable system that allows you to accurately calibrate a biosensor through microfluidic technology. With this system, you can test different solutions (drugs or reagents at different concentrations) in order to calibrate or test your biosensor in an easy way. In addition, this system can be adapted to test any fluid, liquid or gaseous in order to calibrate a biosensor for any application with a sequential fluid Injection system .
AN ALL-IN-ONE PACK TO EASILY TEST YOUR BIOSENSOR
Biosensors are analytical devices that have taken on an important role in the biotechnology sector. This is because they have a wide variety of applications and can be adjusted to different criteria of sensitivity and precision . Biosensors are responsible for converting biochemical signals into signals that can be quantified, which are typically electrical or optical signals. It is for this reason that biosensors must be properly calibrated in order to quantify and minimize the error in each one of them. For this reason, it is necessary to develop an automated system that allows performing different tests to optimize the performance of a biosensor. This is an important activity since one of the most important activities of a biosensor is to obtain accurate information in real-time about the measurements it performs. Also, because a biosensor uses different biological components, it is necessary to take into account a cleaning system between each calibration test.
Why use microfluidics for biosensor testing?
While for a proof of concept, manual testing works very well, this requires a lot of pipetting time that you may want to get rid of for scalability reasons. As soon as you need more accurate professional biosensor testing, an automated system can take you to the next level ensuring better control and accuracy in calibration .
In general, microfluidics will allow you to have high precision because thanks to a microfluidic pump you can control the flow of each sample precisely. This will decrease the variability between results and increase the accuracy obtained in each of the measurements. Similarly, through a microfluidic system, you can decrease the test time by increasing the transport speed of the samples.
In addition, this microfluidic system will allow you to obtain great flexibility since it can be adapted to each biosensor you want to calibrate and at the same time to the specifications of each application. Thanks to the performance of this set of equipment, you will be able to control all the parameters that you need.
KEY ADVANTAGES:
This microfluidic pack allows precise control of the flow through the channel, reducing the amount of sample required and improving detection efficiency
Consistent and uniform sample delivery
Enables you to obtain high reliability for each sensor you want to test
Helps you establish a relationship between the signal produced by the biosensor and the concentration of the measured substance
High reproducibility for testing biosensors for applications in medicine, biotechnology, food, and environmental industries
Overview of the biosensor setup
How to choose the better configuration of the pack?
If you already want to start testing your biosensors you can explore the different configurations that this Elveflow beta pack has for you.
SETUP AND CONTENT:
If you already want to start with the setup of this beta pack you can check the following examples of sensor calibration beta packs on different types of microfluidic chips and type of experiments. In general, these are based on the calibration through the injection of different types of samples thanks to the sequential injection system. For more detailed information, please contact our experts or check our multiple fluid switching application pack.
MODEL of the setup of the pack:
Depending on the intended application of your biosensor it is important that you choose how you want your biosensor testing system to perform. For example, this setup can be used when you want to test a biosensor by varying the input substances.
With our beta pack you will be able to test different liquids in your biosensor. However, if you then wish to vary the concentration, you can consult our beta pack for concentration calibration in biosensors.
Each Pack is composed of:
Hardware:
Contact our experts to answer any questions about this biosensor testing pack and how it can match your specifications!
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sequential vs parallel testing
What tests can I carry out to characterize my biosensor?
To perform any experiment involving a biosensor it is important to first test its performance . This activity will allow you to determine how sensitive and reliable your sensor will be to different samples. To determine the functionality of your biosensor it is necessary to determine some parameters such as:
Once you have decided which parameters are most important for your research you can move on to using your microfluidic device along with a customized Elvwflow pack. All these parameters can be tested easily and automatically through our all-in-one kit. Normally, the main applications of this beta pack are based on the characterization of different samples that can be further enhanced thanks to the MUX distribution.
Concentration variation:
One of the many applications of this kit is based on the use of a large number of sample concentrations. That way you can test parameters such as Concentration measure, Range of concentrations or Response time . These tests are important to know the sensitivity of your biosensor.
For example, you can place up to four biosensors (one biosensor for each channel) thanks to our OB1 pressure controller. It is important to mention that thanks to the high customization provided by this beta pack, these biosensors can be different or the same so that you can ensure the repeatability of your testing. Then, you can join up to 12 samples of different sizes and administer them sequentially to your biosensors.
This way, you can have automated testing of your biosensors in simple steps, compared to using a conventional system that will only allow you to test one sample on a biosensor per step. Therefore, with the biosensor testing pack you will optimize the testing time as well as the amount of samples you need. Microfluidic testing only requires tiny volumes of samples or reagents, so you can easily run different tests and automate them with each other.
Sequential testing
Parallel testing
It is important to note that because biosensors treat biological samples , it is necessary to have a buffer cleaning step between each test. This process generates more accurate and closer to reality results; however, it increases the testing process if a conventional calibration method is used.
For that reason, with the Elveflow beta pack you can save processing time as the buffer cleaning process can be easily included in the pack.
BUFFER CLEANING IN SEQUENTIAL testing
BUFFER CLEANING IN Parallel testing
One of the most common applications for biosensors is based on the variation of the type of drug that these sensors can measure. Thanks to the beta test pack you can vary the type of drug to be tested to evaluate which one has a better performance for your experiment. Also, at the same time you can decide whether to test the performance of different biosensors as you can add up to 4 biosensors for simultaneous testing. The variation of drugs in experimentation has a high relevance and needs an accurate testing system like the one offered by this beta pack since this type of system can be used for the detection of drugs in biological fluids such as sweat, saliva, blood or urine. In addition, it can be used to ensure safety in the quality control process in drug production or in the food industry.
If you wish to go further with the testing of your biosensor you can consult our MUX cross chip and contact our experts who will help you determine which beta pack fits your needs.
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[question] => What kind of biosensors can be adapted to this beta pack?
[reponse] =>
The microfluidic testing system for biosensors can be used to calibrate biosensors by delivering different solutions to the sensor. For this reason, there are different types of biosensors that can be tested with a microfluidic calibration system. For example, one of them can be biochemical biosensors, which bind substances to generate reactions to detect a specific substance. Electrochemical biosensors, which use electrical signals to detect the presence of substances, can also be used. Similarly, surface plasmon resonance biosensors can be used for testing since the substances that can be measured must be varied in order to calibrate it. These sensors use the interaction of light with a metal surface to detect the presence of a given substance. In addition, optical biosensors that use optical signals (such as absorbance, fluorescence or refraction) can be used to detect different concentrations of the substance of interest.
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[question] => Is it possible to parallelize the testing?, What are the possible configurations that can be given to the setup of this beta pack?
[reponse] =>
Yes, parallelization can be achieved with this beta pack and will allow you to simultaneously analyze multiple samples or detect multiple analytes. By incorporating multiple samples a biosensor can increase its performance and efficiency, making it suitable for multiplexed analysis or simultaneous monitoring. This will allow you to have faster analysis of multiple samples, reduce the time required for data collection, and improve the overall sensitivity and specificity of the biosensor.
With our system it will be easier for you to obtain data so that you can obtain a calibration curve for your biosensor. Depending on the intended use of your biosensor, you can apply different types of configurations. For instance, if you want to test multiple sensors at the same time, you can add a manifold to distribute the inlet fluid to all the biosensors. Additionally, it is possible to add a valve to maximize the use of each pressure channel.
Moreover, it is possible to integrate a mixing chip before biosensor testing to perform concentration tests with different substances, as the chip will allow the fluids you choose to combine properly.
Furthermore, it is possible to add an external buffer substance input to have better control over the cleaning of each part of the system between each test.
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[question] => Why do I need all these instruments? Is this pack upgradable?
[reponse] =>
The real-time feedback on the Flow rate pressure and pressure of fluids will be useful to optimize the production process and troubleshoot any issues that arise. This all-in-one pack will guarantee you good compatibility between the different instruments of the pack as they are all controlled by a single software. For this reason, you will be able to use the system quickly to start your experiment right away and focus it on a wide variety of different applications . This feature makes Elveflow packs the best choice as it will allow you to configure your entire microfluidic experiment and customize it according to your needs. Our experts will be able to support you during the whole configuration process and pack start-up.
Yes, this beta pack can be used together with any other Elveflow equipment since they are all compatible with each other. This feature allows you to adapt this pack to your needs.
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[question] => Can I sterilize my lines?
[reponse] =>
Sterilization in biosensor testing is important to ensure the accuracy, reliability and safety of the tests you perform. In that sense, having good contaminant management can help you ensure reproducibility and comparability of results. For that reason, this beta pack considers the buffer cleaning steps in each test cycle of your biosensors. However, for specific applications just contact our expert to check if your method is suitable with our instruments or follow the protocol they will share with you.
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[question] => How much volume do I need to test this?
[reponse] =>
Elveflow equipment is ready for you to use in a wide range of volumes, ranging from microliters to liters. However, to find out which Elveflow equipment and features match your applications, contact our experts.
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CUSTOMIZE YOUR BETA PACK
Elveflow’s products and Beta packs are fully customizable so they will be perfectly adapted to your needs. Our specialists and researchers can help you choose the best instruments and accessories. In addition, they will accompany you during the setup of the microfluidic platform until you can get the experimental results you need.
Elveflow can provide you with a wide range of different flow sensors, bubble detectors and traps or any relevant microfluidic instrument for the applications you need.
Please contact our experts for any questions about this pack and how it can fit your specifications.
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A standard Cell & Biology Pack setup uses one pumping channel to flow multiple solutions into the microfluidic chip. In combination with the flow sensor (MFS or BFS series), the OB1 flow controller allows to have a very stable medium perfusion. Moreover, the medium change is easily done using the MUX Distribution that allows to switch between 12 solutions . All these operations can be performed using only the ESI software interface. The software allows you to fine tune the flow parameters as well as to automate your experiment using the intuitive scheduler .
Advantages
Control both pressure & flow rate : Ideal for shear stress assays
Perform fast switches between media or drugs : For imaging cell response to various media or drugs
Stable & pulseless flow rate : No more coverslip expansion and cell stress
Large range of flow rates : From 10nL/min to 5 mL/min
Design flow injection sequences : Create complex patterns, such as oscillating flow to mimic physiological conditions
Recirculation loop : Ideal for long assays
Instantaneous stop flow : For controlled solution exposure experiments, such as calcium imaging
Our dedicated pack can be adapted for more complex and advanced Cell & Biology experiments such as using 20 solutions, choosing the right microfluidic chip, removing bubbles or to have multiple chip/inlets perfusion.
Contact us for more information!
Computer: Control all the parameters with our software, and automate your experiment by creating injection sequences.
Pressure & flow controller: Impose a given pressure in order to create a stable and pulseless flow rate.
Splitter: Multiple your reservoir’s pressure inlet from a single controller pressure output using our splitter
Reservoirs: Contain your medium or samples. Various sizes are available, from Eppendorf to bottles.
Rotative valve | Mux Distribution: Select the injected liquid.
Flow Sensor: Monitor and control the flow rate in real time.
Perfusion chamber or microfluidic chip: Elveflow can help choosing a microfluidic chip fitting your cell culture requirements .
Microfluidic calculator to estimate the shear stress in your cell culture chamber
To help you determine your flow rate, pressure to apply, the best tubing resistance length for your setup, wall shear stress for biology applications, cell culture, and many more…
Elveflow provides its microfluidic calculator and…to make the most of our microfluidic calculator, find below a set of dedicated application notes :
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BIOLOGY-RELATED FIELDS OF APPLICATION:
Cell confinement assays
Dynamic single cell screening
Drug screening
Cell response to medium change
Live cell imaging
C.elegans stimulation
Cell culture on chip
Toxicity tests
3D cell culture
Bioreactor studies
Stem cells assays
Cells entrapment
Publications
A. Chen et al ., “Single Cell Mass Spectrometry with a Robotic Micromanipulation System for Cell Metabolite Analysis ,” in IEEE Transactions on Biomedical Engineering , doi: 10.1109/TBME.2021.3093097.
Proton gradients from light-harvesting E. coli control DNA assemblies for synthetic cells , Kevin Jahnke et al. Nature Communications (2021). DOI: 10.1038/s41467-021-24103-x
A microfluidic device enabling drug resistance analysis of leukemia cells via coupled dielectrophoretic detection and impedimetric counting , Yağmur Demircan Yalçın et al., Scientific Reports (2021). DOI: 10.1038/s41598-021-92647-5
Highly sensitive wide-range target fluorescence biosensors of high-emittance metasurfaces , Masanobu Iwanaga, Biosensors and Biolectronics (2021). DOI: 10.1016/j.bios.2021.113423
Critical Frequency and Critical Stretching Rate for Reorientation of Cells on a Cyclically Stretched Polymer in a Microfluidic Chip , Jiandong Ding et al., ACS Applied Materials & Interfaces (2021). DOI: 10.1021/acsami.0c21186
In vitro skin model for characterization of sunscreen substantivity upon perspiration , Fatemeh Keshavarzi et al., International Journal of Cosmetic Science (2021). DOI: 10.1111/ics.12703
Electrokinetic sandwich assay and DNA mediated charge amplification for enhanced sensitivity and specificity , Siddharth Sourabh Sahu et al. Biosensors & Bioelectronics (2021). DOI: 10.1016/j.bios.2020.112917
The method to dynamically screen and print single cells using microfluidics with pneumatic microvalves , Chang Chen et al., MethodX (2020). DOI: 10.1016/j.mex.2020.101190
Cyclic on-chip bacteria separation and preconcentration , Vitaly Ryzhkov et al., Scientific Reports (2020). DOI: 1038/s41598-020-78298-y
Synthetic Biology Bicistronic Designs Support Gene Expression Equally Well in vitro and in vivo, Owen Koucky et al., American Journal of Undergraduate Research (2020). DOI: 10.33697/ajur.2020.012
Zhu Z., Geng Y., Wang Y. (2021) Monitoring Single S. cerevisiae Cells with Multifrequency Electrical Impedance Spectroscopy in an Electrode-Integrated Microfluidic Device . In: Marchisio M.A. (eds) Computational Methods in Synthetic Biology. Methods in Molecular Biology, vol 2189. Humana, New York, NY. DOI: 10.1007/978-1-0716-0822-7_9
Investigating the Interaction Between Circulating Tumor Cells and Local Hydrodynamics via Experiment and Simulations
Pepona, M., Balogh, P., Puleri, D.F. et al. . Cel. Mol. Bioeng. (2020). DOI: 10.1007/s12195-020-00656-7
A drug-compatible and temperature-controlled microfluidic device for live-cell imaging , Open Biology; Jul, 2016; T. Chen et al; DOI : 10.1098/rsob.160156
A microfluidic gradient generator to simulate the oxygen microenvironment in cancer cell culture; Microelectronic Engineering; Aug, 2018; Louise Orcheston-Findlay et el; DOI : 10.1016/j.mee.2018.04.011
Geometric Friction Directs Cell Migration; Physical Review Letters; Jul, 2013; Le Berre et al; DOI : 10.1103/PhysRevLett.111.198101
An Integrated Microfluidic Chip and Its Clinical Application for Circulating Tumor Cell Isolation and Single‐Cell Analysis ; Cytometry; Oct, 2019; Mingxin Xu et al; DOI : 10.1002/cyto.a.23902
Mitotic Rounding Alters Cell Geometry to Ensure Efficient Bipolar Spindle Formation; Developmental Cell; Apr, 2013; Oscar M. Lancaster et al; DOI : 10.1016/j.devcel.2013.03.014
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The amazing benefits of microfluidics can be applied to many Cell & Biology perfusion experiments and applications. Therefore, the content of the Cell & Biology Pack can be adjusted to suit your specific needs. Contact us for more details.
Additional pumping channels
Additional flow rate sensors
Microfluidics chips
Computer
Microscope and camera
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[testimonial_text] => I appreciate rapid setup time and the fact that we can very precisely adjust the flow rates through the user-friendly interface. The dynamic and responsive engineering team was an additional benefit.
[testimonial_author] => Dr. Caglar Elbuken, UNAM Bilkent University, Turkey
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[testimonial_text] => We really appreciate the stability and low response time of the flow controller, which is important when various flow conditions must be applied successively. The flow can be precisely and easily controlled thanks to a user-friendly interface.
[testimonial_author] => Dr. Marie Frénéa-Robin, Laboratoire AMPERE Université Claude Bernard LYON 1, France
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[testimonial_text] => Elveflow provided an excellent solution to our problem, which was how to tightly control minute flows in multiple microfluidic channels. Elveflow’s support has been excellent and software development is continuing.
[testimonial_author] => Dr. Robert Ward, Massey University, United States
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[testimonial_text] => Overall we are satisfied with the equipment and highly recommend it to anyone who is working in the microfluidics field.
[testimonial_author] => Dr. Weiqiang Chen, Biomechanics Laboratory (IBBL) University of Michigan, United States
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[testimonial_text] => I was suprised how quickly I was able to get things to work once I got going.
[testimonial_author] => Jamie Stover, Research Assistant, M. Zernicka-Goetz Lab at California Institute of Technology (Caltech)
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Several experiments or tests can be carried out with one system at once so as to automate your multiple liquid injection / testing and save time !
Elveflow’s sequential fluid injection pack is dedicated to any system that requires to quickly swap between several fluids while maintaining a precise flow rate . This makes it a perfect fit for your complex organic chemistry reactions , organic synthesis , biosensors, biochemical sensors or electrochemical sensors, test rigs, flow chemistry, Seq-Fish experiment, analytical chemistry setup, drug and toxicity testing, particle synthesis, organocatalysis experiment, mother machine for antibiotic resistance testing and many more…
This pack is suitable for both biological and chemical experiments such as multiple perfusion switch of liquid media and automated sequential reagent injection.
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This pack includes all the necessary elements to sequentially inject up to 12 (or more on request) solutions in a fully automated fashion using our computer-controlled 12 to 1 MUX Distribution bidirectional valve , high accuracy OB1 flow controller , and intuitive ESI control software.
A large flow rate range (from 7 nL/min to 30+ mL/min) and volumes (100 µL to up to several Liters) are accessible with this system.
The typical Sequential fluid injection pack setup uses only one single pressure channel to sequentially flow multiple solutions into your microfluidic system. Medium change can then easily be carried out using the MUX Distribution ‘s rotary valve that acts as a liquid selector and allows fast switching and selection between your solutions.
In combination with an MFS or BFS flow sensors, the OB1 flow controller allows an extremely stable liquid perfusion (down to 0.006% of the flow rate) .
All these operations can be performed using the ESI software interface. The software allows you to fine-tune the flow parameters as well as to automate your experiment using our intuitive scheduler .
Advantages
A large number of samples: Possibility to sequentially inject up to 12 (or more) solutions in your system
Stable & pulseless flow rate : Very stable fluid delivery for most accurate results thanks to our pressure-driven flow controller
High versatility : Use it with any fluidic system such as microfluidic chip, sensor test rigs, flow chemistry setup
Perform fast switches between media : Switching time below 150 ms
For small and large volume for long term experiments: Work with volumes from few µL to several liters
Control both pressure & flow rate : For maximum versatility on your fluid injection
A broad range of flow rates accessible : From 7nL/min to 30+ mL/min with an accuracy down to 0,2%
Design flow injection sequences : Create complex patterns and fully automatize your system to test any working conditions over hours or days
High parallelization possibilities: Test multiple devices with one setup
Software development kits: in C, LabView, Matlab, and Python for integration into your platform.
How to perform microfluidic multiple liquid switching?
More information on how to perform a stable and precise flow switch in our example application note.
Our sequential fluid injection pack can be adapted for more complex and advanced experiments such as using 20 or more solutions , removing bubbles , integration into larger systems or testing multiple chip/devices simultaneously…
Contact us to know more!
Computer: Control all the parameters with our software, & automate your experiment by creating injection sequences
Pressure & flow controller: Impose a given pressure in order to create a stable and pulseless flow rate
Manifold: Split the pressure path to pressurize multiple reservoirs with a single pressure controller
Reservoirs: Contain your medium or samples. Various sizes are available, from Eppendorf to bottles
Rotary valve: Select the injected liquid
Flow Sensor: Monitor & control the flow rate in real-time
Microfluidic chip: any fluidic system can be hooked up to our fast fluid switching pack
To discover more tips and tricks…
Claim our complete User guide about Automated Fluid Sequential Injection !
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The sequential fluid injection pack can be used in various fields of application and for any experiment that require successive injection of fluids: from organic synthesis, flow chemistry, d rug screening , biochemical and electrochemical sensor calibration, c alibration (bench testing), sensors test rig, toxicity test, Seq-Fish experiment…
Publications
Adaptable pulsatile flow generated from stem cell-derived cardiomyocytes using quantitative imaging-based signal transduction
T. Qian et al. , Lab on a Chip , 2020, 20 , 3744-3756, DOI: 10.1039/D0LC00546K
Microfluidic platform for 3D cell culture with live imaging and clone retrieval
C. Mulas et al., Lab on a chip, 2020, 20 , 2580-2591, DOI: 10.1039/D0LC00165A
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The amazing benefits of microfluidics can be applied to almost any application and therefore the content of the Sequential fluid injection Pack can be adjusted to suit your specific needs. Contact us for more details .
Additional pumping channels
Additional flow rate sensors
Microfluidic chip
Computer
Manifold
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Multiple Liquid switch videos
Related microfluidics webinars
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[testimonial_text] => The auto-calibration function for the flow control is really nice, after that the response is very fast and the flow is stable. We played a bit with different diameter tubing, but I've worked with microfluidic setups before, and it was all very intuitive and easy to use. I already prefer this system over our syringe pumps
[testimonial_author] => Pr. Thomas Hermans, Laboratoire des systèmes complexes hors équilibres - Institut de Science et d'Ingénierie Supramoléculaires (ISIS) - Université de Strasbourg, France
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Single-Molecule Localization Microscopy (SMLM) for High-Resolution Imaging
Single-molecule localization microscopy (SMLM) is a cutting-edge technique designed to explore the intricate structures of biological systems with unparalleled resolution. At the forefront of this innovation is DNA-PAINT (DNA-based point accumulation for imaging in nanoscale topography), a rapidly evolving fluorescence super-resolution method.
DNA-PAINT leverages the stochastic and transient binding of fluorescently labeled DNA probes to achieve exceptional spatial resolution, often below 10 nm . This technique is particularly advantageous for imaging multiple targets within the same sample, making it a powerful tool for researchers.
Initially, DNA-PAINT faced challenges such as low throughput, long acquisition times, and difficulties in integrating with live-cell imaging. However, recent advancements have overcome these obstacles, enabling faster image acquisition and more efficient DNA-PAINT imaging, even in complex live-cell environments.
Our versatile, computer-controlled microfluidic setup is designed to streamline multiplexed DNA-PAINT , making it more accessible and efficient. By utilizing base pairing between short, fluorescently labeled DNA oligonucleotides, our system allows for precise and continuous imaging. The use of modified DNA nucleotide or amino acid-based backbones further enhances the reliability of the technique, addressing challenges related to binding frequency and imaging speed.
Whether you’re studying fixed cells, live cells, or tissues, our DNA-PAINT setup offers a comprehensive solution for high-resolution imaging, helping you achieve breakthrough results in your research.
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DNA-PAINT Pack Applications
DNA-based point accumulation for imaging in nanoscale topography (DNA-PAINT) is a powerful tool in biological research, offering numerous applications. It is particularly valuable for studying genome organization, gene expression, and chromatin structure. As a proof of concept, the DNA-PAINT setup was effectively used to label and image proteins on mitochondria, the Golgi apparatus, and chromatin. This approach was demonstrated in the study titled “Nanobody Detection of Standard Fluorescent Proteins Enables Multi-Target DNA-PAINT with High Resolution and Minimal Displacement Errors” by Shama Sograte-Idrissi et al. [1].
DNA-PAINT enables researchers to visualize specific DNA sequences within cells and tissues, providing critical insights into the genome’s structure and function.
Examples of DNA-PAINT Applications
Genome Organization: DNA-PAINT allows researchers to study the spatial organization of the genome within the nucleus. By using fluorescent probes to label specific DNA sequences, the technique visualizes their location and interactions with other genomic regions, providing insights into nuclear architecture.
Gene Expression: This technique can track gene expression patterns by labeling RNA transcripts with fluorescent probes. It enables the visualization of the location and dynamics of these transcripts within the cell, offering a detailed understanding of gene regulation.
Chromatin Structure: DNA-PAINT is used to explore the structure of chromatin, the DNA-protein complex forming chromosomes. Researchers can label specific chromatin regions to study their organization and behavior within the nucleus, advancing knowledge of chromatin dynamics.
Diagnosis and Disease Research: The technique is valuable in diagnostics and disease research. By designing probes to target disease-associated DNA sequences, DNA-PAINT detects and analyzes genomic changes linked to various diseases, aiding in understanding and diagnosis.
Nanotechnology: DNA-PAINT also finds applications in nanotechnology, such as creating DNA-based nanodevices and biosensors. Researchers can use DNA as a scaffold to construct complex, precisely controlled structures and devices for various technological applications.
References
1. Sograte-Idrissi, S., Oleksiievets, N., Isbaner, S., Eggert-Martinez, M., Enderlein, J., Tsukanov, R., & Opazo, F. (2019). Nanobody detection of standard fluorescent proteins enables multi-target DNA-PAINT with high resolution and minimal displacement errors. Cells, 8(1), 48.
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Customizable DNA-PAINT Setup for High-Resolution Imaging
This illustrative setup example for DNA-PAINT is highly adaptable, allowing you to adjust the number and volume of solutions based on your experimental needs and protocols. The setup is designed to enhance your research with precise control, high reproducibility, and seamless automation.
A typical DNA-PAINT pack includes:
Our solution is fully configurable to meet your specific requirements. Our experts are available to help you define the best possible setup for your research goals. The versatility of the instruments and the upgradability of our range ensure that the pack can be tailored to your exact needs.
Key Advantages:
Achieve ultra-precise flow control of small dispensed volumes.
Synchronize with additional equipment, such as a fluorescence microscope .
Improve the reproducibility of your experiments.
Implement a fast and easy sequential injection system for multiple solutions.
Automate your experiments* for enhanced efficiency.
Visualize multiple genomic regions simultaneously.
Achieve high specificity and spatial resolution in DNA sequence analysis.
*The sequence scheduler allows for the easy automation of your platform, managing the flow of a large number of solutions with minimal effort. Our experts can assist with TTL trigger integration or direct software integration via SDK or UART communication.
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At small scale, liquid handling can be controlled precisely with the help of microfluidics .
This all-in-one system for the sequential injection of liquids/medium is based on two centerpieces: a pressure controller and a rotary valve.
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Our liquid handling pack enables to sequentially inject different liquids. It contains: a MUX Distribution bidirectional valve which works as a selector, a high accuracy flow controller , and Elveflow’s intuitive ESI control software.
The pack also contains a manifold to pressurize several reservoirs using a single pressure channel from the OB1 and a flow sensor to monitor and control the flow rate in real-time.
The MUX distribution rotary valve is characterized by:
Valve flexibility: 12 inlets-1 outlet or 1 inlet-12 outlets
Easy setup and operation: plug and play software and standard fluidic connection
Possibility to choose the sense of rotation
The versatility of the system in terms of fluids.
If your experiment requires the use of a buffer in large quantities but also expensive reagents in tiny volumes, it is not an issue for our liquid handling pack. You can actually connect tanks with various volumes, from bottle to Eppendorf.
Moreover, there is no restriction concerning the use of liquids as the wetted materials of the system are resistant to aggressive reagents. The flow sensors can also be chosen to work with liquids of different properties .
Advantages
Quickly swap your biological media or chemical solutions and inject them thanks to the MUX Distribution valve
Flow can be tuned to match any requirements (pressure, flow rate, injected volumes)
Automation of the whole system
Enables both flow and pressure control when used with a flow sensor
Possible to handle both small (µL) and large volumes of liquids (Liters)
Multiple parameters control (volumes, time of injection…)
Various options for sample collection
Works with any liquids (water, oil…)
Very large flow rate range depending on the flow sensor: from nL/min to mL/min
Automation and integration
Scale up: injection of numerous solutions (up to about 150 solutions)
Customization
How to perform microfluidic multiple liquid switching?
More information on how to perform a stable and precise flow switch in our example application note.
Our liquid handling pack can be adapted for more complex and advanced experiments such as using 20 or more solutions , removing bubbles , integration into larger systems or testing multiple chip/devices simultaneously…
Contact us to know more!
Complete Userguide | Automated Liquid Handling
To discover more tips and tricks…
Get in touch to claim our complete Userguide about Liquid handling & Sequential Injection!
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The liquid handling pack can be used in various fields of application and for any experiment that require successive injection of liquids:
Flow chemistry / lab on chip / functionalization of sensors: The sequential injection is interesting in applications related to flow chemistry, lab on a chip, and functionalization of sensors.
Calibration of sensors / biosensors: The sequential injection makes it possible to automate and to reduce the duration of testing.
Droplets: The combination of sequential injection and droplets can make experiments in drug delivery, sensitivity tests, screening, microfluidic PCR and gradient assay easier.
Brouzes et al, 2009
Cell biology / cell culture: Experiments such as cell response to medium changes, live cell imaging, drug testing, 3D cell culture and spatial transcriptomics that can be improved and easily automated.
Sukenik et al, 2017
Reviews
Microfluidics as a tool for drug delivery
Electrochemistry and Microfluidics : A short review
Microreactors & Microfluidics in Chemistry: a Review
Microfluidic Nanoparticle Synthesis: A short review
See all reviews
Publications
Adaptable pulsatile flow generated from stem cell-derived cardiomyocytes using quantitative imaging-based signal transduction
T. Qian et al. , Lab on a Chip , 2020, 20 , 3744-3756, DOI: 10.1039/D0LC00546K
Microfluidic platform for 3D cell culture with live imaging and clone retrieval
C. Mulas et al., Lab on a chip, 2020, 20 , 2580-2591, DOI: 10.1039/D0LC00165A
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The amazing benefits of microfluidics can be applied to almost any application and therefore the content of the liquid handling pack can be adjusted to suit your specific needs. Contact us for more details .
CONTENT
Selecting the best flow control method for your application is very important, since the performance and fluid handling efficiency of a microfluidic device depends highly on the flow control system. If you require to use our pack for the previously mentioned applications, the solution will contain:
Pressure & flow controller ( OB1 )
Software and SDK libraries (C++, Python, MATLAB, LabVIEW)
You can choose from the following additional options:
Additional pumping channels
Microfluidic valves (2-way and 3-way valves)
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Multiple Liquid switch videos
Related microfluidics webinars
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[testimonial_text] => The auto-calibration function for the flow control is really nice, after that the response is very fast and the flow is stable. We played a bit with different diameter tubing, but I've worked with microfluidic setups before, and it was all very intuitive and easy to use. I already prefer this system over our syringe pumps
[testimonial_author] => Pr. Thomas Hermans, Laboratoire des systèmes complexes hors équilibres - Institut de Science et d'Ingénierie Supramoléculaires (ISIS) - Université de Strasbourg, France
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[testimonial_text] => I have made first experiments and obtained some extremely valuable results.
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Credit: Red blood cells forming rouleaux. Jonathan Armstrong. Licence: Attribution-NonCommercial 4.0 International (CC BY-NC 4.0)
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viscosity testing
what is it?
The Viscosity Testing or viscometry is a technique used to measure the viscosity of a certain fluid. It is a method employed in fluid mechanics and rheology to quantify the resistance of a fluid to deform for flow. The major importance of an accurate viscosity measurement is that it will provide insight into the behavior of fluids.
why microfluidics?
Viscosity is a very important parameter in many different types of research and applications. For that reason, parameter accuracy and parameter control is important. Microfluidics is one of the most valuable methods to measure viscosity for the following reasons:
KEY advantages:
Microfluidic systems (microliter to nanoliter) facilitate faster measurements and lower the cost per experiment.
Microfluidic channels reduce thermal gradients and allow adequate heat transfer.
This technology can be easily combined with other analytical or image processing techniques. This feature allows simultaneous measurements and provides a more complete understanding of the behavior of fluid properties.
Microfluidics reduces the amount of sample required for viscosity measurement, making this technique cost-effective, fast and accurate compared to conventional macroscopic methods.
Microfluidic systems allow a high degree of automation.
Overview of the viscosity testing setup
How to choose the better configuration of the pack?
If you already want to start testing your biosensors you can explore the different configurations that this use case has for you.
SETUP AND CONTENT:
Hardware:
One OB1 flow controller DUAL
Flow sensor MFS (see also the BFS-Coriolis flow sensor for the best performance)
Sets of flow resistors (for stable and accurate flow control)
Rotative distribution valve (MUX distributor )
Mux Wire
One Microchip customized or purchased from ChipShop, a leading manufacturer of microfluidic chips (Y-shaped chip flow laminarly)
Kit starter pack Luer Lock with fittings and tubing
ESI control software (automated sequences possible
The combination of these instruments is the fastest and most precise microfluidic flow control available on the market which guaranties the best measurements.
Contact our experts to answer any questions about this biosensor testing pack and how it can match your specifications!
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CUSTOMIZE YOUR PACK
Elveflow’s products and packs are fully customizable so they will be perfectly adapted to your needs. Our specialists and researchers can help you choose the best instruments and accessories. In addition, they will accompany you during the setup of the microfluidic platform until you can get the experimental results you need.
Elveflow can provide you with a wide range of different flow sensors, bubble detectors and traps or any relevant microfluidic instrument for the applications you need.
Please contact our experts for any questions about this pack and how it can fit your specifications.
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All-in-one system for automating fluid viscosity measurement
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[texte] =>
What is a microfluidic pressure-driven flow controller?
Cutting-edge Microfluidic Flow Controller
Designed by scientists for scientists, the versatile and powerful OB1 MK4 pressure controller provides the perfect flow control for all kinds of applications.
Whether you need pressure or vacuum, low or high flow rate, for short or week-long processes and experiments, the OB1 MK4 is the ideal instrument for your microfluidic needs.
The best performance on the market: Piezoelectric Technology
The Elveflow OB1 MK4 is one of the best performing microfluidic flow control instruments worldwide that uses piezoelectric regulators. The piezoelectric technology gives you 20 times more precise and 10 times faster flow control than any other flow controller on the market.
Customizable & upgradable: 1 module, up to 4 channels, 5 pressure & vacuum ranges available
The OB1 MK4 can be configured according to your needs. In one piece of equipment, you can have up to 4 pressure and/or vacuum channels (and other customized options).
If your needs change, the instrument can be upgraded later, in any way you want.
Get the fastest flow rate control when
paired with a flow sensor
Connect the OB1 MK4 to a standard liquid flow rate sensor
(MFS )
or our premium Coriolis flow sensor
(BFS , suitable for both liquid and gas)
to directly control the flow rate in your chip. The system continuously calculates the pressure and maintains the desired and constant flow rate.
Full control software, SDK, and UART communication
Single and intuitive software to get started in a few clicks and automate complex and long experiments.
The SDK libraries allow you to control the OB1 MK4 using your own code while connecting it to other instruments.
The MK4 is also equipped with UART communication protocol in addition to the ESI and SDKs control, allowing it to communicate with most control systems, such as Mac, Linux, Arduino, PLC.
OEM version available
The OB1 MK4 can be used on a bench setup or embedded in your own product.
Elveflow has a solution for every step of your research & development.
Discover our
OB1 MK4 OEM solution.
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[texte] => Applications of Pressure-Driven Flow Control
Thanks to its ultra-fast response time, high stability, and precise flow control, the OB1 pressure controller is a preferred choice for a wide range of liquid handling microfluidic applications, compared to syringe pumps or peristaltic pumps. Combined with precision flow sensors (like MFS or BFS ), the OB1 pressure controller can accurately control flow rate in any microfluidic setups. Below is an overview of how pressure-driven flow control improves research across different domains.
Droplet & Bead Generation
Droplet Microfluidics
Flow control microfluidics offers unmatched stability and responsiveness when generating monodisperse droplets in microchannels. The precise flow control is essential for the generation of identical droplets avoiding most post-synthesis steps. This monodispersity is essential in applications like digital PCR, single-cell analysis, or encapsulation. Explore droplet generation setup
Alginate Bead Generation
In biomedical research, alginate beads are used to encapsulate cells or molecules. Pressure-driven systems allow fine-tuned control over flow rates, ensuring uniform bead size and reliable encapsulation efficiency. See the alginate bead pack.
Biology & Cell Culture
Cell Perfusion
Pressure-based perfusion ensures bubble-free, pulsation-free delivery of nutrients or drugs to cultured cells over extended periods, ideal for mimicking physiological flow conditions. Discover perfusion setup
Organ-on-a-Chip
Simulating organ-level responses on chips requires controlled fluid environments. Pressure-driven systems provide the accuracy and reactivity needed to replicate dynamic flow patterns and shear stress. Learn about Organ-on-a-Chip systems
Cell Confinement Assays
Experiments that study how cells adapt to physical constraints benefit from stable pressure control to reproducibly apply mechanical stress. See confinement assay example
Cell Constriction Assays
Revealing cellular responses to fibrotic environments or disease models requires reproducible deformation of cells, enabled by finely tuned pressure-controlled flows. View ischemia model example
Cell Trapping & Synthetic Compartments
Pressure-driven flows help trap and localize cells or materials in adapted microfluidic chips, useful in synthetic biology or compartmentalization studies. Read the synthetic cells study
Flow Control & Mixin
Sequential Injection & Mixing
Delivering fluids in a precise sequence or generating on-chip gradients requires fast and programmable flow transitions. Pressure controllers enable seamless transitions between multiple fluid inlets. Explore the injection pack
Recirculation
Some in vitro assays require long term dynamic flow of reagents or cell culture media. In most cases it is essential to reuse the media, in order to mimic physiological conditions or avoid expenses. Using pressure-based flow control, one-way recirculation is possible to program for long term experiments. Learn about the recirculation setup
Analytical Chemistry & Imaging
Flow Chemistry
Pressure-driven systems provide accurate reagent dosing and fast startup/shutdown, essential for reaction optimization in continuous-flow chemistry. This is particularly useful in the development of immunoassays and biosensors. See flow chemistry application
Liquid-Phase Electron Microscopy (LPEM)
Stable, pulseless flow is critical for imaging samples in real time at the nanoscale. Pressure control allows precise delivery of fluids during live-cell imaging under an electron beam, especially to study samples in their natural environment. Explore LPEM research
Light-Field Flow Cytometry (LFC)
In high-throughput single-cell analysis, stable and controlled flow conditions are key to accurate imaging and data capture. Pressure-based systems enable high-speed and consistent sample handling. Learn about 3D LFC
Industrial Applications
Enhanced Oil Recovery (EOR)
Microfluidic chips simulating porous media allow researchers to visualize oil displacement mechanisms. Pressure control enables fine adjustment of flow to mimic subsurface conditions with high reproducibility. Read the full EOR review
Cosmetic Formulation & Testing
Microfluidics offers new possibilities for precision formulation of cosmetic emulsions and nano-encapsulation of active ingredients. Pressure-driven flow ensures reproducibility and scalability for testing stability, texture, or skin permeability on-chip. Explore cosmetic applications
Integrated Systems
Lab-on-a-Chip Platforms
At the core of any lab-on-a-chip system is the need for precise, responsive, and stable flow control, features inherently supported by pressure-driven microfluidics. Whether it’s diagnostics, environmental sensing, or point-of-care testing, the OB1 system provides the control backbone required for success. Discover some lab-on-chip examples in this review
Videos
Publications
For all publications featuring our products, please click here .
For all application notes based on our product, please click here .
For reviews of our product, please click here .
)
[specifications] => Array
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[texte] =>
Channel pressure range
0 to 200 mbar(1)
(0 to 2.9 psi)
0 to 2,000 mbar(1)
(0 to 29 psi)
0 to 8,000 mbar(1)
(0 to 116 psi)
-900 to 1,000 mbar(1)
(-13 to 14.5 psi)
-900 to 6,000 mbar(1)
(-13 to 87 psi)
Pressure stability(2)
0.015 % FS(1)
0 µbar (0.0004 psi)
0.005 % FS(1)
100 µbar (0.0014 psi)
0.006 % FS(1)
500 µbar (0.007 psi)
-900 to 500 mbar
-900 to 2,000 mbar
0.005 % FS(1)
100 µbar (0.0014 psi)
0.005 % FS(1)
350 µbar (0.005 psi)
500 to 1,000 mbar
2,000 to 6,000 mbar
0.007 % FS(1)
150 µbar (0.0021 psi)
0.007 % FS(1)
525 µbar (0.0076 psi)
Response time(3)
Down to 10 ms
Setting time(4)
Down to 50 ms
Minimum pressure increment
0.006 % FS(1)
12 µbar (0.00017psi)
0.006 % FS(1)
120 µbar (0.0017 psi)
0.006 % FS(1)
480 µbar (0.007 psi)
0.0064 % FS(1)
120 µbar (0.0017 psi)
0.0064 % FS(1)
420 µbar (0.006 psi)
Pressure supply
1.5 bar (or Max pressure + 0.5 bar) to 10 bar
Non corrosive, non explosive, dry and oil-free gases, e.g. air, argon, N2, CO2, …
Input vacuum(5)
/
/
Any value from -0.7 to -1 bar
Compatible with vacuum pump or vacuum line
Input pneumatic connector
6 mm push-in
Output pneumatic connector
4 mm push-in
Liquid compatibility
Non contact pump
Any aqueous, oil, or biological sample solution.
Flow sensor compatibility
Compatible with the whole MFS and BFS range Monitoring and feedback loop flow control available
Flow sensor compatibility
From 0,1 µL/min to 500 mL/min (indicative, please refer to the MFS and BFS series)
Flow sensor compatibility
Non-contact pump Any aqueous, oil, or biological sample solution.
Software control
Elveflow Smart Interface – Windows 10, 11, both 32 and 64 bit versions supported
Software Development Kit
Libraries available: Matlab, Python, LabView, C++
Flow sensor compatibility
Non-contact pump Any aqueous, oil, or biological sample solution. – Windows 10, 11, both 32 & 64 bit versions supported Serial/UART communication protocol on request
Data management
Possibility to log and extract data (CSV), channel and sensor detailed information using ESI
Input profiles
Possibility to load profiles: ramp, sine, triangle, square, or custom
Automation
Generate step-by-step sequences using the ESI built-in sequence management Log and export custom configurations (CSV)
Screen
LCD screen showing pressure and sensor flow rate in real time
Voltage
24V
Typical power
12W
Power connection
DC power connector, Plug, 3A, 2.1mm, 12mm
Interface
USB Type B
Communication type
Serial
Sensor connection
M8 4-pins, one connector per channel
Compatibilit
Elveflow sensors: MFS, BFS, MPS, MFP, MBD Custom sensors: 5 to 24V supply voltage, 0 to 10V readout voltage
Triggers
Input and Output TTL signal 0V or 5V
Supply Voltage Range (V)
100 to 240 VAC
Supply AC Frequency (Hz)
50 to 60 Hz
Maximum Output Current (A)
1.67 A
Maximum Output Power (W)
40 W
Dimensions (length x width x height)
240 x 223 x 83 mm
Weight
1.4 kg to 2.9 kg
(1) Max pressure value might vary by +/- 2.5% (2) Pressure stability (standard deviation) measured over the full pressure range with an external high accuracy pressure sensor (Druck DPI150) (3) Time required to reach 5% of the setting point. Depending on your computer’s operating system (4) Time required to reach 95% of the set point. Volume dependent – Measurement was done on 12 mL reservoir for a set point from 0 to 200 mbar (5) A vacuum source is mandatory for calibration and use of dual channels even if the channels are to be used in pressure only.
[image] =>
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[0] => Array
(
[question] => What pressure range should I choose?
[reponse] =>
The performances of the OB1 (for example, the pressure stability) is tied to the full operating pressure range.
In order to achieve optimum performance, we recommend our users to choose the smallest pressure range that covers the required experimental pressures.
)
[1] => Array
(
[question] => Can I achieve direct flow control?
[reponse] =>
By coupling a MFS or BFS flow sensor to the OB1 flow controller, you can use the software to directly control the flow rate by directly inputting the flow rate value.
The software uses a PID loop to control the flow rate by setting automatically the pressure to reach the requested flow rate. Doing so, you can benefit of the best of the two worlds to get a fast and precise (pressure driven) volume flow rate (flow sensor).
)
[2] => Array
(
[question] => Can I connect a Pressure Sensor to the OB1? -
[reponse] =>
Pressure and flow sensors can be connected directly to the OB1 MK4. The sensors can be used in passive mode (for monitoring only) or active mode (to enslave).
)
[3] => Array
(
[question] => Do I need a pressure and/or vacuum source to operate the OB1 Pressure controller ?
[reponse] =>
Yes, you will need a pressure source to work with the OB1 pressure controller.
You can either use the air supply of your lab if there is one, use a compressed air bottle or use an air compressor. Elveflow has selected a compressor that is perfectly fitted for most application using the OB1.
If your OB1 has a vacuum channel, you will need a vacuum pump. Elveflow also proposes a selected pump that work perfectly with the OB1.
)
[4] => Array
(
[question] => How to install the OB1?
[reponse] =>
Click here
Unboxing video
)
)
)
[more] => Array
(
[more_title] => Software
[texte] =>
Software
ESI is Elveflow’s dedicated software interface, built to make life easier for experimenters. It is perfectly adapted for the control of simple and complex setups and workflow automation. It integrates several modules that make time-consuming and painful tasks simple. It comes with SDK libraries to control the Elveflow system from your own code.
The OB1 control window allows to:
Visualize the key parameters of each channel:
the command pressure
the output pressure
Visualize sensor output when one is connected to the OB1 (a flow rate sensor on the first channel in the video presented below)
Set any pressure or flow rate pattern (predefined or custom)
Easily switch to the flow rate control mode (sensor mode)
Use the pause/play button to preset the parameters on each channel and launch them simultaneously
Save configurations to gain time in the future or automate your workflow thanks to the sequencer
Open a graph to visualize and save data
Launch and use dedicated modules to simplify specific experimental tasks
Which sensor is compatible with OB1 MK4?
[image] =>
[video] =>
)
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[text] =>
Advantages of Pressure control in microfluidics
Fast settling time
High stability and pulseless flow
Possibility to handle fluid volumes of several liters
Enable both flow and pressure control when used with a flow meter
For more details about Pressure driven flow control, please read this application note .
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[texte_1] =>
Directly connect a flow sensor
Control the flow inside your microfluidic setup
By connecting a MFS or BFS Flow Sensor to the OB1 MK4, you enable direct rapid and precise regulation of the flow rate. The PID algorithm of the software reads the flow rate and adjusts the pressure accordingly to reach the target flow rate with speed and precision.
[texte_2] =>
5 pressure ranges for each channel
Choose the pressure range best suited to your need
We have several pressure channel options available covering a wide range, up to 8000 mbar (116 PSI) and down to -900 mbar (-13 PSI). Choose the pressure channel that covers your experimental pressure requirements.
[texte_3] =>
Choose between 1 to 4 channels, and more…
How many channels do you want?
The OB1 MK4 is a compact module that can accommodate up to 4 channels (pressure and/or vacuum). Each module can be upgraded at any time and have channels added or changed. You can control as many channels as you want with one computer.
Need more channels on one module (8, 16, 64…. or even more)? Our custom service would design a specific pressure controller that fits your needs.
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[testimonial_text] => We are using the device extensively at the moment and I have been quite impressed by its capabilities
[testimonial_author] => Dr. Hayden Taylor
[testimonial_product] => OB1 flow controller user
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[ID] => 97261
[title] => Flow controller
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[post_type_name] => Flow control system
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Flow control systems 1 pages found
1 to 4 channels pressure vacuum microfluidic flow controller
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