contact@elveflow.com+33(0).184.163.807+1(414)-406-4343 Elveflow is an Elvesys brand, an international microfluidics innovation center with high level researchers dedicated to microfluidics and organ-on-a-chip.
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Image Credit: J. Adam Fenster / University of Rochester. Licence: Attribution 4.0 International (CC BY 4.0)
Image Credit: Norovirus – colour-enhanced. David Gregory & Debbie Marshall. Licence: Attribution 4.0 International (CC BY 4.0)
This beta pack is dedicated to the test, characterization, and calibration of biosensors with small volumes, reproducibility, and automatization. We have sought to create a system that unifies and automates the tests necessary to calibrate any biosensor based on Elveflow instruments.
The main objective of this pack is to create a scalable system that allows you to accurately calibrate a biosensor through microfluidic technology. With this system, you can test different solutions (drugs or reagents at different concentrations) in order to calibrate or test your biosensor in an easy way. In addition, this system can be adapted to test any fluid, liquid or gaseous in order to calibrate a biosensor for any application with a sequential fluid Injection system.
AN ALL-IN-ONE PACK TO EASILY TEST YOUR BIOSENSOR
Biosensors are analytical devices that have taken on an important role in the biotechnology sector. This is because they have a wide variety of applications and can be adjusted to different criteria of sensitivity and precision. Biosensors are responsible for converting biochemical signals into signals that can be quantified, which are typically electrical or optical signals. It is for this reason that biosensors must be properly calibrated in order to quantify and minimize the error in each one of them. For this reason, it is necessary to develop an automated system that allows performing different tests to optimize the performance of a biosensor. This is an important activity since one of the most important activities of a biosensor is to obtain accurate information in real-time about the measurements it performs. Also, because a biosensor uses different biological components, it is necessary to take into account a cleaning system between each calibration test.
While for a proof of concept, manual testing works very well, this requires a lot of pipetting time that you may want to get rid of for scalability reasons. As soon as you need more accurate professional biosensor testing, an automated system can take you to the next level ensuring better control and accuracy in calibration.
In general, microfluidics will allow you to have high precision because thanks to a microfluidic pump you can control the flow of each sample precisely. This will decrease the variability between results and increase the accuracy obtained in each of the measurements. Similarly, through a microfluidic system, you can decrease the test time by increasing the transport speed of the samples.
In addition, this microfluidic system will allow you to obtain great flexibility since it can be adapted to each biosensor you want to calibrate and at the same time to the specifications of each application. Thanks to the performance of this set of equipment, you will be able to control all the parameters that you need.
If you already want to start testing your biosensors you can explore the different configurations that this Elveflow beta pack has for you.
If you already want to start with the setup of this beta pack you can check the following examples of sensor calibration beta packs on different types of microfluidic chips and type of experiments. In general, these are based on the calibration through the injection of different types of samples thanks to the sequential injection system. For more detailed information, please contact our experts or check our multiple fluid switching application pack.
Depending on the intended application of your biosensor it is important that you choose how you want your biosensor testing system to perform. For example, this setup can be used when you want to test a biosensor by varying the input substances.
With our beta pack you will be able to test different liquids in your biosensor. However, if you then wish to vary the concentration, you can consult our beta pack for concentration calibration in biosensors.
Each Pack is composed of:
Hardware:
Contact our experts to answer any questions about this biosensor testing pack and how it can match your specifications!
To perform any experiment involving a biosensor it is important to first test its performance. This activity will allow you to determine how sensitive and reliable your sensor will be to different samples. To determine the functionality of your biosensor it is necessary to determine some parameters such as:
Once you have decided which parameters are most important for your research you can move on to using your microfluidic device along with a customized Elvwflow pack. All these parameters can be tested easily and automatically through our all-in-one kit. Normally, the main applications of this beta pack are based on the characterization of different samples that can be further enhanced thanks to the MUX distribution.
For example, you can place up to four biosensors (one biosensor for each channel) thanks to our OB1 pressure controller. It is important to mention that thanks to the high customization provided by this beta pack, these biosensors can be different or the same so that you can ensure the repeatability of your testing. Then, you can join up to 12 samples of different sizes and administer them sequentially to your biosensors.
This way, you can have automated testing of your biosensors in simple steps, compared to using a conventional system that will only allow you to test one sample on a biosensor per step. Therefore, with the biosensor testing pack you will optimize the testing time as well as the amount of samples you need. Microfluidic testing only requires tiny volumes of samples or reagents, so you can easily run different tests and automate them with each other.
It is important to note that because biosensors treat biological samples, it is necessary to have a buffer cleaning step between each test. This process generates more accurate and closer to reality results; however, it increases the testing process if a conventional calibration method is used.
For that reason, with the Elveflow beta pack you can save processing time as the buffer cleaning process can be easily included in the pack.
One of the most common applications for biosensors is based on the variation of the type of drug that these sensors can measure. Thanks to the beta test pack you can vary the type of drug to be tested to evaluate which one has a better performance for your experiment. Also, at the same time you can decide whether to test the performance of different biosensors as you can add up to 4 biosensors for simultaneous testing. The variation of drugs in experimentation has a high relevance and needs an accurate testing system like the one offered by this beta pack since this type of system can be used for the detection of drugs in biological fluids such as sweat, saliva, blood or urine. In addition, it can be used to ensure safety in the quality control process in drug production or in the food industry.
If you wish to go further with the testing of your biosensor you can consult our MUX cross chip and contact our experts who will help you determine which beta pack fits your needs.
Yes, parallelization can be achieved with this beta pack and will allow you to simultaneously analyze multiple samples or detect multiple analytes. By incorporating multiple samples a biosensor can increase its performance and efficiency, making it suitable for multiplexed analysis or simultaneous monitoring. This will allow you to have faster analysis of multiple samples, reduce the time required for data collection, and improve the overall sensitivity and specificity of the biosensor.
With our system it will be easier for you to obtain data so that you can obtain a calibration curve for your biosensor. Depending on the intended use of your biosensor, you can apply different types of configurations. For instance, if you want to test multiple sensors at the same time, you can add a manifold to distribute the inlet fluid to all the biosensors. Additionally, it is possible to add a valve to maximize the use of each pressure channel.
Moreover, it is possible to integrate a mixing chip before biosensor testing to perform concentration tests with different substances, as the chip will allow the fluids you choose to combine properly.
Furthermore, it is possible to add an external buffer substance input to have better control over the cleaning of each part of the system between each test.
Yes, this beta pack can be used together with any other Elveflow equipment since they are all compatible with each other. This feature allows you to adapt this pack to your needs.
Sterilization in biosensor testing is important to ensure the accuracy, reliability and safety of the tests you perform. In that sense, having good contaminant management can help you ensure reproducibility and comparability of results. For that reason, this beta pack considers the buffer cleaning steps in each test cycle of your biosensors. However, for specific applications just contact our expert to check if your method is suitable with our instruments or follow the protocol they will share with you.
Elveflow equipment is ready for you to use in a wide range of volumes, ranging from microliters to liters. However, to find out which Elveflow equipment and features match your applications, contact our experts.
Elveflow’s products and Beta packs are fully customizable so they will be perfectly adapted to your needs. Our specialists and researchers can help you choose the best instruments and accessories. In addition, they will accompany you during the setup of the microfluidic platform until you can get the experimental results you need.
Elveflow can provide you with a wide range of different flow sensors, bubble detectors and traps or any relevant microfluidic instrument for the applications you need.
Please contact our experts for any questions about this pack and how it can fit your specifications.
Elveflow® provides a perfusion system dedicated to cell culture, for lab-on-chips, flow cells and perfusion chambers. This pack includes all the necessary elements to create a continuous flow and monitor flow rate applied on the cells.
Ideal for experiments requiring switches between different cell culture media. A computer-controlled valve allows sequential injections (up to 12 different medium or reagents, more on demand).
The intuitive ESI control software allows to quickly automate your complex experimental workflows.
A standard Cell & Biology Pack setup uses one pumping channel to flow multiple solutions into the microfluidic chip. In combination with the flow sensor (MFS or BFS series), the OB1 flow controller allows to have a very stable medium perfusion. Moreover, the medium change is easily done using the MUX Distribution that allows to switch between 12 solutions. All these operations can be performed using only the ESI software interface. The software allows you to fine tune the flow parameters as well as to automate your experiment using the intuitive scheduler.
Our dedicated pack can be adapted for more complex and advanced Cell & Biology experiments such as using 20 solutions, choosing the right microfluidic chip, removing bubbles or to have multiple chip/inlets perfusion.
Contact us for more information!
To help you determine your flow rate, pressure to apply, the best tubing resistance length for your setup, wall shear stress for biology applications, cell culture, and many more…
Elveflow provides its microfluidic calculator and…to make the most of our microfluidic calculator, find below a set of dedicated application notes:
Learn more on dynamic cell culture using this pack.
Learn how to automate cell seeding with this pack.
Learn more on long-term medium perfusion using this pack
The amazing benefits of microfluidics can be applied to many Cell & Biology perfusion experiments and applications. Therefore, the content of the Cell & Biology Pack can be adjusted to suit your specific needs. Contact us for more details.
Credit: Red blood cells forming rouleaux. Jonathan Armstrong. Licence: Attribution-NonCommercial 4.0 International (CC BY-NC 4.0)
Viscosity is a very important parameter in many different types of research and applications. For that reason, parameter accuracy and parameter control is important. Microfluidics is one of the most valuable methods to measure viscosity for the following reasons:
If you already want to start testing your biosensors you can explore the different configurations that this use case has for you.
The combination of these instruments is the fastest and most precise microfluidic flow control available on the market which guaranties the best measurements.
Put different types of measure of viscosity
Elveflow’s products and packs are fully customizable so they will be perfectly adapted to your needs. Our specialists and researchers can help you choose the best instruments and accessories. In addition, they will accompany you during the setup of the microfluidic platform until you can get the experimental results you need.
An all-in-one flow control system for fast switching of fluids while maintaining a defined flow rate.
Several experiments or tests can be carried out with one system at once so as to automate your multiple liquid injection / testing and save time!
Elveflow’s sequential fluid injection pack is dedicated to any system that requires to quickly swap between several fluids while maintaining a precise flow rate. This makes it a perfect fit for your complex organic chemistry reactions, organic synthesis, biosensors, biochemical sensors or electrochemical sensors, test rigs, flow chemistry, Seq-Fish experiment, analytical chemistry setup, drug and toxicity testing, particle synthesis, organocatalysis experiment, mother machine for antibiotic resistance testing and many more…
This pack is suitable for both biological and chemical experiments such as multiple perfusion switch of liquid media and automated sequential reagent injection.
This pack includes all the necessary elements to sequentially inject up to 12 (or more on request) solutions in a fully automated fashion using our computer-controlled 12 to 1 MUX Distribution bidirectional valve, high accuracy OB1 flow controller, and intuitive ESI control software.
A large flow rate range (from 7 nL/min to 30+ mL/min) and volumes (100 µL to up to several Liters) are accessible with this system.
The typical Sequential fluid injection pack setup uses only one single pressure channel to sequentially flow multiple solutions into your microfluidic system. Medium change can then easily be carried out using the MUX Distribution‘s rotary valve that acts as a liquid selector and allows fast switching and selection between your solutions.
In combination with an MFS or BFS flow sensors, the OB1 flow controller allows an extremely stable liquid perfusion (down to 0.006% of the flow rate).
All these operations can be performed using the ESI software interface. The software allows you to fine-tune the flow parameters as well as to automate your experiment using our intuitive scheduler.
More information on how to perform a stable and precise flow switch in our example application note.
Our sequential fluid injection pack can be adapted for more complex and advanced experiments such as using 20 or more solutions, removing bubbles, integration into larger systems or testing multiple chip/devices simultaneously…
Contact us to know more!
To discover more tips and tricks…
Get in touch to claim our complete Userguide about Automated Fluid Sequential Injection!
The sequential fluid injection pack can be used in various fields of application and for any experiment that require successive injection of fluids: from organic synthesis, flow chemistry, drug screening, biochemical and electrochemical sensor calibration, calibration (bench testing), sensors test rig, toxicity test, Seq-Fish experiment…
For more biology-related content, please refer to the cell perfusion application pack.
Adaptable pulsatile flow generated from stem cell-derived cardiomyocytes using quantitative imaging-based signal transduction
T. Qian et al., Lab on a Chip, 2020, 20, 3744-3756, DOI: 10.1039/D0LC00546K
Microfluidic platform for 3D cell culture with live imaging and clone retrieval
C. Mulas et al., Lab on a chip, 2020, 20, 2580-2591, DOI: 10.1039/D0LC00165A
The amazing benefits of microfluidics can be applied to almost any application and therefore the content of the Sequential fluid injection Pack can be adjusted to suit your specific needs. Contact us for more details.
At small scale, liquid handling can be controlled precisely with the help of microfluidics.
This all-in-one system for the sequential injection of liquids/medium is based on two centerpieces: a pressure controller and a rotary valve.
The pressure-driven flow controller allows higher stability, better response time compared to systems using a peristaltic pump or a syringe pump,
This liquid handling pack is simple to set-up and to operate and enables easy system automation.
The platform can be adapted to any requirements and fully automated.
Our liquid handling pack enables to sequentially inject different liquids. It contains: a MUX Distribution bidirectional valve which works as a selector, a high accuracy flow controller, and Elveflow’s intuitive ESI control software.
The pack also contains a manifold to pressurize several reservoirs using a single pressure channel from the OB1 and a flow sensor to monitor and control the flow rate in real-time.
The MUX distribution rotary valve is characterized by:
If your experiment requires the use of a buffer in large quantities but also expensive reagents in tiny volumes, it is not an issue for our liquid handling pack. You can actually connect tanks with various volumes, from bottle to Eppendorf.
Moreover, there is no restriction concerning the use of liquids as the wetted materials of the system are resistant to aggressive reagents. The flow sensors can also be chosen to work with liquids of different properties.
Our liquid handling pack can be adapted for more complex and advanced experiments such as using 20 or more solutions, removing bubbles, integration into larger systems or testing multiple chip/devices simultaneously…
Get in touch to claim our complete Userguide about Liquid handling & Sequential Injection!
The liquid handling pack can be used in various fields of application and for any experiment that require successive injection of liquids:
The amazing benefits of microfluidics can be applied to almost any application and therefore the content of the liquid handling pack can be adjusted to suit your specific needs. Contact us for more details.
Selecting the best flow control method for your application is very important, since the performance and fluid handling efficiency of a microfluidic device depends highly on the flow control system. If you require to use our pack for the previously mentioned applications, the solution will contain:
You can choose from the following additional options:
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microscale. ) [insert] => wpdrop [header__content___sidebar_ads2] => Array ( [image__header_ads2] => [subtitle_text__header_ads2] => [title__header_ad_ads2] => [sub_title_2_ads2] => [content__text__ad_ads2] => [link__header_ads2] => ) [utiliser_acf] => [most_popular] => [review_type] => 11 [citation_author_repeater] => [citation_publication_date] => [citation_keywords_repeater] => [description_GS] => [ID] => 143381 [title] => Emerging Trends in Microfluidics [permalink] => https://www.elveflow.com/microfluidic-reviews/general-microfluidics/emerging-trends-in-microfluidics/ [post_type] => reviews [post_type_name] => Review ) [8] => stdClass Object ( [preview] => Array ( [image] => Array ( [ID] => 13684 [id] => 13684 [title] => CReaNet microfluidics chemical reaction mixing Elvesys color [filename] => CReaNet_microfluidics_chemical_reaction_mixing_-Elvesys_color.jpg [filesize] => 91107 [url] => 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[ID] => 629 [title] => OPTICAL DETECTION TECHNIQUES FOR MICROFLUIDICS [permalink] => https://www.elveflow.com/microfluidic-reviews/general-microfluidics/optical-detection-techniques-microfluidics/ [post_type] => reviews [post_type_name] => Review ) [14] => stdClass Object ( [preview] => Array ( [image] => Array ( [ID] => 593 [id] => 593 [title] => free copyright microfluidic researcher [filename] => free-copyright-microfluidic-researcher.jpg [filesize] => 74971 [url] => https://www.elveflow.com/wp-content/uploads/2019/08/free-copyright-microfluidic-researcher.jpg [link] => https://www.elveflow.com/microfluidic-reviews/microfluidic-flow-control/researchers-opinion-on-flow-control-for-microfluidics/free-copyright-microfluidic-researcher/ [alt] => free copyright microfluidic researcher [author] => 27 [description] => free copyright microfluidic researcher [caption] => free copyright microfluidic researcher [name] => free-copyright-microfluidic-researcher [status] => inherit [uploaded_to] => 592 [date] => 2019-08-28 14:40:09 [modified] => 2024-08-19 14:21:17 [menu_order] => 0 [mime_type] => image/jpeg [type] => image [subtype] => jpeg [icon] => https://www.elveflow.com/wp-includes/images/media/default.png [width] => 1280 [height] => 960 [sizes] => Array ( [thumbnail] => https://www.elveflow.com/wp-content/uploads/2019/08/free-copyright-microfluidic-researcher-150x150.jpg [thumbnail-width] => 150 [thumbnail-height] => 150 [medium] => https://www.elveflow.com/wp-content/uploads/2019/08/free-copyright-microfluidic-researcher-300x225.jpg [medium-width] => 300 [medium-height] => 225 [medium_large] => https://www.elveflow.com/wp-content/uploads/2019/08/free-copyright-microfluidic-researcher-768x576.jpg [medium_large-width] => 768 [medium_large-height] => 576 [large] => https://www.elveflow.com/wp-content/uploads/2019/08/free-copyright-microfluidic-researcher-1024x768.jpg [large-width] => 1024 [large-height] => 768 [1536x1536] => https://www.elveflow.com/wp-content/uploads/2019/08/free-copyright-microfluidic-researcher.jpg [1536x1536-width] => 1280 [1536x1536-height] => 960 [2048x2048] => https://www.elveflow.com/wp-content/uploads/2019/08/free-copyright-microfluidic-researcher.jpg [2048x2048-width] => 1280 [2048x2048-height] => 960 ) ) [texte] => One of the great challenges for researchers using microfluidics is miniaturizing analysis processes in very small microchip. ) [utiliser_acf] => [most_popular] => [review_type] => 9 [insert] => ob1fc2 [citation_author_repeater] => Array ( [0] => Array ( [citation_author] => The Elveflow team ) ) [citation_publication_date] => 2021/01/06 [citation_keywords_repeater] => Array ( [0] => Array ( [citation_keywords] => flow control ) ) [description_GS] => One of the great challenges for researchers using microfluidics is miniaturizing analysis processes in very small microchip. 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The history of microfluidics is strictly related to several other areas ) [utiliser_acf] => [most_popular] => [review_type] => 11 [insert] => starter2 [citation_author_repeater] => Array ( [0] => Array ( [citation_author] => Alessandra Delaquilla ) ) [citation_publication_date] => 2021/02/05 [citation_keywords_repeater] => Array ( [0] => Array ( [citation_keywords] => history of microfluidics ) ) [description_GS] => Today, MICROFLUIDICS is a distinct and major technological field, but 20 years ago it was not like this and its boundaries were not so well defined. 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However, controllable and fast mixing is critical for microfluidic and lab-on-chip devices. So different mixing techniques were developed and are here presented. ) [utiliser_acf] => [most_popular] => [review_type] => 9 [insert] => secinjection2 [citation_author_repeater] => Array ( [0] => Array ( [citation_author] => Guilhem Velvé Casquillas ) [1] => Array ( [citation_author] => Timothée Houssin ) ) [citation_publication_date] => 2019/07/09 [citation_keywords_repeater] => Array ( [0] => Array ( [citation_keywords] => Microfluidic mixers ) [1] => Array ( [citation_keywords] => lab-on-chip devices ) [2] => Array ( [citation_keywords] => assays ) ) [description_GS] => Microfluidic mixers: controllable and fast mixing is critical for subsequent practical development of microfluidic and lab-on-chip devices and assays [header__content] => Array ( [image__header] => [subtitle_text__header] => [title__header_ad] => [content__text__ad] => [link__header] => ) [header__content___sidebar_ads2] => Array ( [image__header_ads2] => [subtitle_text__header_ads2] => [title__header_ad_ads2] => [sub_title_2_ads2] => [content__text__ad_ads2] => [link__header_ads2] => ) [ID] => 596 [title] => Microfluidic mixers : a short review [permalink] => https://www.elveflow.com/microfluidic-reviews/microfluidic-flow-control/microfluidic-mixers-a-short-review/ [post_type] => reviews [post_type_name] => Review ) [17] => stdClass Object ( [preview] => Array ( [image] => Array ( [ID] => 140504 [id] => 140504 [title] => OB1-microfluidics-pressure-driven-flow-controller-backflow-filters [filename] => OB1-microfluidics-pressure-driven-flow-controller-backflow-filters.webp [filesize] => 658004 [url] => https://www.elveflow.com/wp-content/uploads/2024/06/OB1-microfluidics-pressure-driven-flow-controller-backflow-filters.webp [link] => https://www.elveflow.com/microfluidics-application-packs/microfluidics-packs/automated-sample-dispensing-use-case/ob1-microfluidics-pressure-driven-flow-controller-backflow-filters-3/ [alt] => elveflow OB1 [author] => 51 [description] => [caption] => [name] => ob1-microfluidics-pressure-driven-flow-controller-backflow-filters-3 [status] => inherit [uploaded_to] => 140196 [date] => 2024-06-06 15:21:13 [modified] => 2025-01-23 16:24:57 [menu_order] => 0 [mime_type] => image/webp [type] => image [subtype] => webp [icon] => https://www.elveflow.com/wp-includes/images/media/default.png [width] => 1000 [height] => 618 [sizes] => Array ( [thumbnail] => https://www.elveflow.com/wp-content/uploads/2024/06/OB1-microfluidics-pressure-driven-flow-controller-backflow-filters-150x150.webp [thumbnail-width] => 150 [thumbnail-height] => 150 [medium] => https://www.elveflow.com/wp-content/uploads/2024/06/OB1-microfluidics-pressure-driven-flow-controller-backflow-filters-300x185.webp [medium-width] => 300 [medium-height] => 185 [medium_large] => https://www.elveflow.com/wp-content/uploads/2024/06/OB1-microfluidics-pressure-driven-flow-controller-backflow-filters-768x475.webp [medium_large-width] => 768 [medium_large-height] => 475 [large] => https://www.elveflow.com/wp-content/uploads/2024/06/OB1-microfluidics-pressure-driven-flow-controller-backflow-filters.webp [large-width] => 1000 [large-height] => 618 [1536x1536] => https://www.elveflow.com/wp-content/uploads/2024/06/OB1-microfluidics-pressure-driven-flow-controller-backflow-filters.webp [1536x1536-width] => 1000 [1536x1536-height] => 618 [2048x2048] => https://www.elveflow.com/wp-content/uploads/2024/06/OB1-microfluidics-pressure-driven-flow-controller-backflow-filters.webp [2048x2048-width] => 1000 [2048x2048-height] => 618 ) ) [texte] => There are three main classes of systems to control liquid motion in microfluidic and nanofluidic devices. ) [utiliser_acf] => [most_popular] => [review_type] => 9 [insert] => ob1fc2 [citation_author_repeater] => Array ( [0] => Array ( [citation_author] => The Elveflow team ) ) [citation_publication_date] => 2021/01/06 [citation_keywords_repeater] => Array ( [0] => Array ( [citation_keywords] => flow control microfluidics ) ) [description_GS] => There are three main classes of systems to control liquid motion in microfluidic and nanofluidic devices. 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This review focuses on point of care (POC) diagnostic devices for pathogen detection
Lab-On-a-Chip drug testing in Microfluidics
Electrochemical detection is particularly suitable for lab-on-a-chip integration and microfluidics due to the versatility of size, geometry and nature of electrodes that can be integrated within a microfluidic platform, and to the minimum instrumentation it requires.
One of the key criteria to choose your microfluidic device material is its chemical resistance. This review will help you choose one depending on your application.
The integration of CRISPR-Cas9 with microfluidics has led to the development of innovative techniques for genetic editing and screening.
Microfluidics is a term which appears more and more often in papers and scientific magazines; but, what exactly is microfluidics?
Cell culture consists in growing cells in an artificial environment in order to study their behavior in response to their environment[1]. Different kinds of cell cultures can be found nowadays, and some would be more suited than others depending on its properties and applications.
Centrifugal microfluidics, or "Lab-on-a-CD," leverages centrifugal force to manipulate fluids on a microscale.
Fluid mixing at microscale is of importance for many fields of application. This short review summarises the key takeaway aspects of fluid mixing at microscale.
A lab-on-a-chip is a miniaturized device that integrates onto a single chip one or several analyses, which are usually done in a laboratory; analyses such as DNA sequencing or biochemical detection.
In this review, we will present the PCR, qPCR and other associated methods with their microfluidic applications.
Introduction to thermoelectric sensor | Microfluidics immunosensors offer multiple advantages over the conventional immunoassays that include improved reaction rate, reduced time for incubation of the reactants, and decreased reagents and sample consumption. Moreover, miniaturization and integration of the multiple assay components permit automation, precise flow control, increased reproducibility, and the possibility for high-throughput analysis.
Microfluidics involve different types of devices, and materials for microfluidic fabrication must be selected while bearing in mind all the requirements necessary for building a fine microfluidic device.
Suitable detection techniques are required to be coupled to microfluidic technology in order to analyze experiment outcomes in a sensitive and scalable way.
One of the great challenges for researchers using microfluidics is miniaturizing analysis processes in very small microchip.
Today, MICROFLUIDICS is a distinct and major technological field, but 20 years ago it was not like this and its boundaries were not so well defined. The history of microfluidics is strictly related to several other areas
In microfluidic laminar flows, traditional turbulent mixing between two liquids cannot occur. However, controllable and fast mixing is critical for microfluidic and lab-on-chip devices. So different mixing techniques were developed and are here presented.
There are three main classes of systems to control liquid motion in microfluidic and nanofluidic devices.
Microfluidic flow cells with embedded optical sensors, have been the most common choice for microenvironment monitoring like pH control.
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Discover the innovative use of elastin-like polypeptide (ELP) coacervates as reversibly triggerable compartments in synthetic cells. Explore how phase separation, dynamic compartmentalization, and lab-on-a-chip techniques redefine synthetic cell engineering.
This short review explores the fabrication of a leak resistant microfluidic chip. Most importantly, the 4 channel PDMS-based microfluidic chip is also tested under pressurized fluid injection for multiple liquid leak resistance checks.
Electrokinetic sandwich assay and DNA mediated charge amplification
Complex droplet networks using pressure-driven microfluidics
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It provides an additional degree of freedom in manipulating both the size and the formation frequency of micro-droplets. 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This application note explains how to set up a robust and reproducible microfluidic platform for liposomes assembly with improved encapsulation efficiency and reduced polydispersity in size.
Active droplet generation in microfluidics is of high interest for a wide range of applications. It provides an additional degree of freedom in manipulating both the size and the formation frequency of micro-droplets. This additional control is extremely desirable for complex operations which rely on the accurate control of both parameters.
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