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Microfluidic Endothelial Cell Culture

Use case

Biologically Active Cellular Monolayer with Stable Hemodynamic Forces
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Microfluidic endothelial cell culture

Transition seamlessly from traditional 2D cell culture to cutting-edge organ-on-chip technology

Dynamic perfusion conditions

Our microfluidic platform provides controlled shear stress with laminar flow, accurately replicating in vivo conditions essential for vascular research, drug toxicity studies, and disease mechanism exploration.

Improved in vitro model

Efficient perfusion replicates physiological conditions, with our system enabling quick substance injection and precise flow control, allowing fine-tuning of strain, shear stress, and pressure for realistic biomechanical environments.

Advanced Microfluidic Endothelial Cell Culture: Achieving a functional endothelial cell layer in a microfluidic channel is now within reach. This use case includes everything you need to create an in vitro model that closely parallels the in vivo environment. Our plug-and-play system is designed to help you move beyond traditional static cultures and into the realm of organ-on-chip technologies.

Complete Setup with Expert Support: Our system is built around the Elveflow OB1 flow controller and a specialized membrane biochip, ensuring accurate flow control and cell culture conditions. With our user-friendly setup and dedicated support, you can easily establish a high-quality endothelial cell culture with enhanced marker protein expression and robust cell adhesion.

Experience in Organ-on-Chip Projects: Our team has extensive experience in organ-on-chip research, having contributed to numerous projects over the years. We’re eager to share our insights and collaborate on new ideas, ensuring your experiments are set up for success.

Microfluidic Endothelial Cell Layer Culture: This use case features a single pumping channel coupled with a distributor, enabling the seeding of different cell types on either side of a membrane within the chip. This configuration facilitates the creation of a more physiologically relevant endothelial cell layer, ideal for developing new therapies or conducting toxicity screenings.

Controlled Perfusion for Optimal Results: Efficient perfusion is critical to replicating physiological conditions. Our system allows for the quick injection of substances, with precise flow control provided by our microfluidic flow sensors. You can fine-tune strain, shear stress, and pressure to create realistic biomechanical environments.

Endothelial cell culture use case setup

Our all-in-one use case ensures seamless compatibility between components, allowing for a quick start to your experiments. Everything is controlled by a single software platform, making it easy to set up and run your microfluidic experiments.

Included Components:

  • OB1 MK4 flow controller (Elveflow)
  • Rotative valve (MUX Distributor)
  • MUX recirculation for medium recirculation
  • Microfluidic flow sensor (e.g., Galileo)
  • Cross-flow membrane chip (suggestion: Fluidic 568)
  • Falcon reservoirs for sample and medium storage
  • 3/2 microfluidic valves
  • MUX wire for valve control
  • Pressure splitter manifold
  • Tubing and connectors
  • Software (Galileo user interface)
  • Primary human umbilical vein endothelial cells (HUVECs) (optional)

Maximized Efficiency with Minimal Sample Use: Microfluidics allows you to minimize the volume of valuable samples required, significantly reducing experiment costs. This precision also enables the replication of in vivo fluid properties at a micro scale, offering a more accurate model for human organ systems.

Enhanced Physiological Relevance: Microfluidic endothelial cell layers provide a more accurate and flexible environment for experiments, closely mimicking in vivo conditions. This makes them far superior to traditional 2D cultures or animal models, particularly as regulatory bodies and the public push for reduced animal testing.

Applications in Drug Testing and Disease Research: Our microfluidic platform allows for more efficient and precise assessments of drug toxicity and pathogen effects on endothelial cells, opening new avenues for therapeutic developments.

Visualizing Endothelial Cell Cultures: Create accurate models of endothelial cell environments, including complex interactions within blood vessels. This setup enables the replication of key mechanical forces and chemical environments that influence vascular research.

Principles of Microfluidic Endothelial Cell Culture: Endothelial cells are central to vascular function and are affected by various chemical, biological, and physical stimuli. Microfluidic models allow researchers to recreate the dynamic conditions of blood vessels, providing a more realistic in vitro environment for studying diseases like diabetes and cancer.

Comparative Studies in Microfluidic and Traditional Cultures: Research has shown that endothelial cells exhibit better morphology, viability, and density when cultured under dynamic flow conditions within microfluidic systems compared to traditional static cultures. These findings underscore the importance of using microfluidic technology for more accurate and reliable vascular research.

Blood-Brain Barrier and Permeability Studies: Microfluidic systems are also being used to model the blood-brain barrier, offering human-relevant insights into neurological diseases. Additionally, researchers can study the permeability of endothelial cell layers under varying shear stress conditions.

Our cross-flow membrane chip can be tailored to your specific needs, with options for materials (Cyclo Olefin Copolymer or Polystyrene) and pore sizes (0.2 µm or 8 µm). Custom membranes are also available upon request.

To prevent issues such as bubble formation during cell culture, we offer solutions like bubble removers. Our experts are available to help you optimize your setup based on your unique requirements.

– Explore our other use cases for a variety of applications –

Microfluidic Endothelial Cell Culture

Use case

Biologically Active Cellular Monolayer with Stable Hemodynamic Forces
Talk to an expert

Microfluidic endothelial cell culture

Transition seamlessly from traditional 2D cell culture to cutting-edge organ-on-chip technology

Dynamic perfusion conditions

Our microfluidic platform provides controlled shear stress with laminar flow, accurately replicating in vivo conditions essential for vascular research, drug toxicity studies, and disease mechanism exploration.

Improved in vitro model

Efficient perfusion replicates physiological conditions, with our system enabling quick substance injection and precise flow control, allowing fine-tuning of strain, shear stress, and pressure for realistic biomechanical environments.

Talk to an expert
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