Recirculation loop setup

Allows a long term perfusion setup with our passive recirculation loop, featuring four check valves that enable unidirectional flow with exceptional stability. Unilateral flow provides more meaningful results compared to traditional back-and-forth flows. Using the state-of-the-art OB1 flow controller, paired with in-line sensors, you can accurately recirculate fluids such as the medium used in dynamic cell cultures. This setup offers precision and reliability, ensuring consistent flow in one direction throughout your experiments. Recirculation can also be integrated with existing instruments, utilizing valves or MUX recirculation for added versatility.

Passive Check Valves	Active Valves
Very light and compact (10 cm²)	Usually bigger and heavier
Can directly go into incubators	Don’t go into incubators
Does not need electric plug or USB connection	Need at least electricity
Very cheap	Expensive
Straightforward to use	Tricky to use for beginners
Easily replaceable/washable	Usually require complicated washing procedure

In this setup, the four passive check valves are arranged to ensure that the flow within the connected microfluidic chip always moves in the same direction, whether pressurizing the first or second reservoir. This method allows for continuous unidirectional flow, enhancing the effectiveness of your microfluidic experiments. The OB1 flow controller, known for its precision and speed, uses flow rate measurements from a flow sensor to create a highly accurate feedback loop, ensuring optimal performance in your recirculation process.