Main applications:
In this application note, we will describe how to perform an automated and fast medium switch thanks to the Perfusion Pack.
Fast sample or medium switch – Real time video
Flow controller OB1 Mk3
MUX Distributor
Sample reservoirs
Tubing
Fittings
Microfluidic device or perfusion chamber
The following diagram shows the microfluidic setup used for this application note.
Pressure & Flow controller: Impose a given pressure in order to create a stable and pulseless flow rate.
Reservoirs: Contain your medium or samples. Various sizes are available, from Eppendorf to bottles.
Rotative valve: Select the injected liquid.
Flow Sensor: Monitor and control the flow rate in live time.
Perfusion chamber or microfluidic chip: Contains your cells. Compatible with microscopy.
Computer: Control all the parameters with our software, and automate your experiment by creating injection sequences.
The MUX distributor allows a simple control of the medium switch. You enter your different solutions inside the software and set which one to inject into the chip. The rotative valve will select your medium in less than one second. If a flow sensor is used in the setup, the software automatically displays the volume of injected liquid for each sample.
The switch can be done by hand but can also be automated thanks to the sequence programmer of the Elveflow software. This allows to obtain a better reproducibility and to plan long experiments. In the following video, a switch every 3 seconds between two liquids has been programmed. Note that no perturbation or air bubble have been introduced during the medium switches.
How can we help you?
Name*
Email*
Message
Newsletter subscription
We will answer within 24 hours
By filling in your info you accept that we use your data.
Do you want tips on how to best set up your microfluidic experiment? Do you need inspiration or a different angle to take on your specific problem? Well, we probably have an application note just for you, feel free to check them out!
Biofilm testing using a simple microfluidic chip channel for in situ observation of their development under flow conditions.
Microfluidics for microscopy imaging in plant biology allows to observe, in vivo, the biological response of plant roots to various stimuli.
A simple guide to do dynamic cell culture by automating cell seeding in a microlfuidic chip
This application note describes how co-culture of different cell types in separate but interconnected chambers is possible in a microfluidic platform
This application note explains how to study bacteria adaptation to stress and environmental changes such as antibiotics.
In this application note we describe how to set up medium recirculation by using microfluidic valves
In this application note we describe how to create a medium recirculation for dynamic cell culture with a microfluidic setup.
In this application note we describe how to stain cells for dynamic cell culture with different microfluidic setups.
In this application note we describe how to do cell perfusion for dynamic cell culture and a way to enable uni-directional recirculation of medium.
This application note proposes a microfluidic cardiac cell culture model (μCCCM) to recreate mechanical loading conditions observed in the native heart (in both normal and pathological conditions) by using an Elveflow OB1 pressure and flow controller.
Medium switch is widely used in cell biology. One application is the study of cell behavior under given flow conditions for different samples. In this tutorial, we walk you through the steps of a fast and stable medium switch using IBIDI© flow cells.
Fluorescence reader for microfluidic qPCR: faster, more sensitive and less expensive than most optical microscopes, it is a smart alternative for real-time fluorescence measurements of your on-chip qPCR signal.
Prostate cancer is the second leading cause of cancer-related death for men. Circulating tumor cells (CTCs) are considered as a marker of early cancer diagnosis and disease severity. Their screening in blood is thus crucial to detect metastatic stage in cancer patients.
Until recently, microfluidic devices have been employed to support tissue-engineering experiments on basal lamina, vascular tissue, liver, bone, cartilage and neurons as well as organ-on-chips.
This application note presents how to perform cell culture on chip using the Cell and Biology Pack and the MicroSlides developed by ALine Inc.
Get a quote
Collaborations
Need customer support?
Serial Number of your product
Support Type AdviceHardware SupportSoftware Support
Subject*
I hereby agree that Elveflow uses my personal data Newsletter subscription
Message I hereby agree that Elveflow uses my personal data Newsletter subscription