SET-UP TIME : 10-15 MIN / DIFFICULTY : *****
This application note shows how to easily perform a controlled drug switch on a hypothetical chemical or biological environment (drug screening or cell culture) using an Elveflow® MUX as shown in these pictures:
For drug switch in perfusion chambers or one inlet microfluidic chips, discover our dedicated Perfusion Pack.
The software version used for this application note is the Elveflow® Smart Interface V2.
You can find each of these elements in the following gallery:
As often as possible avoid using soft tubing (like Tygon) which involves compliance and then increases the response time of the system. If you’re not familiar with microfluidic tubings, you may read our dedicated reviews.
The following diagram illustrates the complete chain of elements involved in this application note. You will need to assemble all these parts to set up your experiment:
Be sure that all the cables and tubing are well connected to your Elveflow devices (USB cable, 24V DC, etc).
Perform leakage tests and remove any air bubbles before starting your experiment to ensure a good flow regulation. Knowing what fitting is best suited for your needs is a first step towards success. If you’re not familiar with microfluidic fittings, you may read our specific reviews.
In order to monitor the pressure to perform an ultrafast medium switch, it is necessary to follow the next steps:
Channel 1 (orange drug): 100 mbar (70% of the channel)
Channel 2 (control medium): 100 mbar (100% of the channel)
Channel 3 (green drug): 90 mbar (30% of the channel)
It is possible to save this configuration for later use by clicking on the “save config” menu option. It is possible to change the channel name by directly editing the channel name display at the left side of the window.
Channel 1 (orange drug): 90 mbar (30% of the channel)
Channel 3 (green drug): 100 mbar (70% of the channel)
If needed, it is possible to see a graph display by clicking on the “open graph display” button. Set the desired maximum and minimum shown parameters on the display window for pressure and flow rate and press the play icon to launch the pressure and flow rate profiles display. Select the channels you want to display by ticking the corresponding boxes on the channel display selection block.
The screenshots corresponding to some of these steps are in the following gallery:
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Do you want tips on how to best set up your microfluidic experiment? Do you need inspiration or a different angle to take on your specific problem? Well, we probably have an application note just for you, feel free to check them out!
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