OPTOREADER – Optical detection for microfluidics

Optical Reader for Microfluidics: the OptoReader brings about the strength of duplex optical fiber measurement, high optical sensitivity and real-time processing capacity within a compact design. It offers the same performance as a microscope while being more cost effective. The use of optical fiber for both illumination and light collection largely frees up the space around the experiment and reduces the optical alignment to only one step.

COMPACT PLATFORM FOR FLUORESCENCE QUANTIFICATION

Attributes

High sample rate: 100 000 samples/s
High sensitivity: refer to the technical specifications
Lock-in detection capability: to eliminate constant background
Compact design: the use of optical fiber allows low footprint and versatile integration
Plug & Play: USB computer connection, empowered by the Elveflow software suite (ESI)
Interoperability: TTL source trigger

Application fields

Microfluidics, fiber optic sensor, analytical or bio chemistry, biology, real-time inspection (food, pharmaceutics, etc), aerospace, etc

Application notes

Multiple configuration possibilities:

Single detection: Measurement of fluorescence or reflection
Dual detection: Simultaneous measurement on two detection channels
Multiple color excitation: beta, up to 3 colors

with

Custum filter set: 9 types of fluorophores
Custom excitation wavelength: from UV to IR

And many other options:

High fluorescence sensitivity: cooled detector, noise lower by a factor 10.
LED or LASER source
Alignment platform: Micrometric alignment table allowing beam alignment in a microfluidic chip.
Inspection camera
Customer inspired: Contact us for original propositions inspired by your needs!

PROPERTIES	SPECIFICATION			Unity
EXCITATION
Excitation wavelength	365, 470, 530, 590, 625			nm
Fluorescence filter Set	DAPI, FITC, TRITC, Texas Red, Cy3, Cy5, etc
Light output intensity	0-1.5			mW
Pulse duration (pulse mode)	10-10⁷			µs
Modulation (Lock-in mode)	0.05 -10			kHz
ACQUISITION
Acquisition frequency	0-100			kHz
Acquisition resolution	16			bits
Typ. acquisition dynamic	84 dB			dB
	Reflection	Fluorescence
Measurement range	0 – 12	0 – 0.12		µW
Noise equivalent power	10^-5	Base	3 x 10^-15	W/Hz^1/2
		Hypersensitive (Analog)	0.3 x 10^-15
		Hypersensitive (Digital)	10	kcps
Photoelectric sensitivity (532 nm)	0.5 x 10⁶	Base	2 x 10⁹	V/W
Photoelectric sensitivity (532 nm)		Hypersensitivity	10¹⁰	V/W
Equivalent fluorescence background (Specified on normal mode, virtually zero in lock-in mode)		< 1 nM FITC
OPTICS
Fiber core diameter	50, 100, 200, 400			µm
Numerical aperture	0.22 – 0.6
Focalized spot diameter	35, 75, 150, 300			µm
MECHANICS
Dimension	35 x 25 x 12			cm³

Detection limit : <1nM FITC (available option with photon counting capability).

Other wavelength and focalized spot diameter available on request.

These specifications are not contractual and can be changed without notice.

USB flow sensor software module

Thanks to an intuitive interface, the Elveflow® Smart Interface enables the use of Elveflow® instruments from the simplest commands for beginners to the most complex manipulations for experts… read more

Control your experiments through C, Python, Matlab®, Labview® or the Elveflow® Smart Interface. The Elveflow® Smart Interface is a software application offering all the functionalities that microfluidicists need.

T. Houssin, J. Cramer, R. Grojsman, L. Bellahsene, G. Colas, H. Moulet, W. Minella, C. Pannetier, M. Leberre, A. Plecis and Yong Chen. Ultrafast, sensitive and large-volume on-chip real-time PCR for the molecular diagnosis of bacterial and viral infections. Lab on a Chip, Feb 2016, DOI: 10.1039/c5lc01459j

Application note Detection of fluorescent droplets using the Optoreader

droplet generation fluorescence measurement optoreader Ob1 — This diagram shows the microfluidic setup used for Detection of fluorescent droplets using Optoreader.

[TS-VCSC-Youtube content_youtube=”https://youtu.be/O0zUN-LxRtA” video_start=”2″ lightbox_play=”true”]

Optical detection microfluidic application chemical reaction

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