Microfluidics application note
How to perform an automated & fast sample switch in a perfusion chamber or microfluidic chip?
Main applications:
In this application note, we will describe how to perform an automated and fast medium switch thanks to the Perfusion Pack.
Fast sample or medium switch – Real time video
Advantages of the setup presented in this application note for cell biology:
LIST OF COMPONENTS
Microfluidic device or perfusion chamber
Setup diagram
The following diagram shows the microfluidic setup used for this application note.
Pressure & Flow controller: Impose a given pressure in order to create a stable and pulseless flow rate.
Reservoirs: Contain your medium or samples. Various sizes are available, from Eppendorf to bottles.
Rotative valve: Select the injected liquid.
Flow Sensor: Monitor and control the flow rate in live time.
Perfusion chamber or microfluidic chip: Contains your cells. Compatible with microscopy.
Computer: Control all the parameters with our software, and automate your experiment by creating injection sequences.
Protocol and results
The MUX distributor allows a simple control of the medium switch. You enter your different solutions inside the software and set which one to inject into the chip. The rotative valve will select your medium in less than one second. If a flow sensor is used in the setup, the software automatically displays the volume of injected liquid for each sample.
Medium switch in a perfusion chamber
Medium switch in a microfluidic chip
The switch can be done by hand but can also be automated thanks to the sequence programmer of the Elveflow software. This allows to obtain a better reproducibility and to plan long experiments. In the following video, a switch every 3 seconds between two liquids has been programmed. Note that no perturbation or air bubble have been introduced during the medium switches.
Microfluidics knowledge
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Medium switch is widely used in cell biology. One application is the study of cell behavior under given flow conditions for different samples. In this tutorial, we walk you through the steps of a fast and stable medium switch using IBIDI© flow cells.
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note
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note
Tissue engineering: Heart Ventricule assisted by a microfluidic pressure pump
Until recently, microfluidic devices have been employed to support tissue-engineering experiments on basal lamina, vascular tissue, liver, bone, cartilage and neurons as well as organ-on-chips.
-
note
Performing live cells assays on chip
This application note presents how to perform cell culture on chip using the Cell and Biology Pack and the MicroSlides developed by ALine Inc.
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