Review Type: Microfluidics for cell biology

Microfluidic cell culture - a beginner friendly review Microfluidic cell culture for beginners Microfluidics is an advanced technique based on multiple disciplines. Hence, getting behind the technique and its underlying principles can be very challenging. Cell biologists have already discovered the potential of microfluidics and are working on cell in microfluidic chambers [1]. Why this … Continued

Microfluidic Cell Culture: a way toward flow cells Traditionally, the culture of live cells outside the body is performed in monolayers inside static polymer-coated plastic flasks placed in CO2 incubators, which maintain a high level of humidity and constant temperature (37 oC), and concentration of CO2 (5%) for pH buffering. The cultures are composed of … Continued

Microfluidics for sperm sorting: a review Written by Audrey Nsamela Published April 22th 2021 Contact: partnership@elvesys.com, Elvesys SAS, 172 Rue de Charonne 75011 Paris Introduction to Microfluidics for sperm sorting Assisted reproductive technologies (ARTs) are very important tools to deal with infertility problems which affect almost 50 million couples around the world. It is estimated that … Continued

Cell biology: Microfluidic Concepts and methodologies Microfluidic technology is creating powerful tools for cell biologists to control the complete cellular microenvironment, leading to new questions and new discoveries. We review here the basic cell biology concepts and methodologies in designing microfluidic devices, and their diverse cell applications. . .1. Introduction to microfluidics for cell biology  … Continued

Perfusion culture for live-cell imaging: A review 1. Perfusion vs static cell culture This short review will present some technical aspects and challenges encountered in perfusion culture for live-cell imaging. Live-cell imaging is a non destructive method which focuses on the observation of live cells and is widely used in cellular biology research and biomedical … Continued

Microfluidic cell separation and sorting: A review Introduction to microfluidic cell sorting and separation Review done thanks to the support of the LAPASO project FP7-PEOPLE-2013-ITN - Marie-Curie Action: "Innovative Training Networks" Written by Walter Minnella, PhD student : walter.minnella@elvesys.com Some standard techniques have been developed for cell separation and sorting. These techniques often require intensive tasks … Continued

Microfluidic chambers for cell culture: How to perform fast medium and drug change? Source: www.mantis.cz/mikrofotografie under Creative Commons license Ability to switch drugs in seconds or less in microfluidic chambers for cell culture allows to study cell response at cells timescale. There are a lot of applications in microfluidic cell culture where the use of … Continued

PDMS in biology research: A critical review on PDMS lithography for biological studies Introduction to PDMS in Biology research PDMS in biology research and more precisely PDMS biocompatibility is an important parameter in many fields of nowadays biology research as microfluidics and lab-on-a-chip is of choice to study various aspects of organ on a chip. … Continued

Methods & techniques for perfusion cell culture I. Dynamic cell culture: WEBINAR For more insight into methods and techniques to perform cell perfusion, please refer to this webinar introducing the concept of dynamic cell culture by incorporating perfusion, and describing the benefits of fluid flow, mechanical tension and shear stress to in vitro cell models … Continued

Imaging and microscopy: Perfusion chambers Introduction about perfusion chambers for live-cell imaging and microscopy The first live-cell imaging chambers were designed in the early twentieth century, shortly after mammalian cell culture techniques were developed. A wide range of perfusion chambersare now available, from simple sealed coverslip on a microscope glass to sophisticated perfusion chamber allowing a … Continued