Application type: Microfluidic cell culture

Biolfim Testing Under Flow Bacteria are able to adhere to most surfaces, and will develop communities over time known as biofilms if the conditions are favorable. Bacterial biofilms are present in many relevant phenomena in our daily lives, both beneficial and detrimental. From the plaque in our teeth to biofouling in ships, chronic infections and … Continued

Microfluidics for microscopy imaging and treatment of Arabidopsis thaliana primary roots in vivo  Microfluidics for microscopy imaging in plant biology allows to observe, in vivo, the biological response of plant roots to various stimuli at high temporal and spatial resolutions. Plants are sessile organisms constantly balancing their internal state with their direct environment to optimize … Continued

Cell-cell interactions on chip Introduction The Interaction Chip (Fluidic 688) enables the co-culture of different cell types in separate but interconnected chambers. The co-culture of different cell types is an important step towards increased physiological relevance for in vitro models of physiology and disease. Vascular networks connect all organ systems, transporting biomolecules from one location … Continued

How to study bacteria adaptation to stress and environmental changes by microfluidics? This application note explains how to study bacteria adaptation to stress and environmental changes with pressure-driven flow controlled microfluidics such as the effect of antibiotics on bacterial growth. It aims to introduce the reader to an experimental method allowing the study of the … Continued

Medium recirculation using microfluidic valves Microfluidic cell culture set-up with valves Dynamic cell culture using microfluidic recirculation valves adds important stimuli that bring in vitro experiments closer to the complexity of physiological conditions. In a living organism, cells are constantly perfused by body fluids, such as blood and interstitial fluids. Therefore, controlling the flow rate … Continued

How to stain cells cultured in a microfluidic chip for dynamic cell culture? Dynamic cell culture Forces are abundant on cells and tissues in the body but are largely absent from traditional cell culture setups, which are static. Microfluidic platforms enable the controlled addition of fluid flow and mechanical stress to cells grown in vitro. … Continued

An easy guide to do microfluidic perfusion for dynamic cell culture Dynamic cell culture Dynamic cell culture describes the in vitro culture of cells in the presence of applied mechanical stress. Of great emerging interest is the ability to control the flow of fluid, such as medium, over cells while they grow. Flow rate can … Continued