A standard Recirculation Pack setup uses two pumping channels to alternatively flow medium from one reservoir to another. An injection valve allows to switch between both reservoirs, to ensure that the medium is pumped from the most filled reservoir. The injection loop consists of a network of inputs/outputs that can be connected to each other in two configurations. Its very low internal volume (2.5 μL) makes it the ideal valve to perform the injection of a controlled volume of sample into a chip.
Recirculation Pack
Microfluidic recirculation pack
Full system for continuous unidirectional recirculation experimentsUnidirectional liquid flow
The liquid stream flows continuously in the same direction
No more media depletion
Don't be limited by your sample volume: recirculate your medium
Uniform Shear Stress
Same flow direction ensures no variations in shear stress
Week-long experiments
Automate your experiments for several days
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Features & Benefits
How to perform fluid recirculation?
The Elveflow® smart interface (ESI) synchronizes the flow controller together with the injection valve in order to perform recirculation. It performs the switch between reservoirs automatically either after a predetermined period of time or, in combination with a the flow rate sensor (MFS), after a chosen amount of sample has gone through the chip. The software is also able to seamlessly run long term experiments thanks to its powerful scheduler.
Additional features such as medium switch, one-off sample injection, etc… are also possible by adding extra Elveflow’s equipment to your Recirculation Pack.
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Microfluidic calculator to estimate the shear stress in your cell culture chamber
To help you determine your flow rate, pressure to apply, the best tubing resistance length for your setup, wall shear stress for biology applications, cell culture, and many more…
Elveflow provides its microfluidic calculator and…to make the most of our microfluidic calculator, find below a set of dedicated application notes:
Publications
Adaptable pulsatile flow generated from stem cell-derived cardiomyocytes using quantitative imaging-based signal transduction
T. Qian et. al, Lab on a Chip, 2020, 20, 3744-3756, DOI: 10.1039/D0LC00546K
Investigation of flow dynamics and structure in channels with structured surfaces
P. Vilkinis, 2020, Doctoral Thesis, Kaunas University of Technology, Lituanian Energy Institute
CONTENT
A standard Recirculation Pack contains the following:
- 2 x OB1 PUMPING CHANNELS
- 1 x FLOW RATE SENSOR
- 1 x Recirculation Valve
- 2 x reservoirs
- All necessary accessories: tubing, connectors, filters, etc…
- CONTROL AND AUTOMATION SOFTWARE
OPTIONS
- Microfluidic cell culture chips: Elveflow is working with several partners who can provide chips specifically designed for certain microfluidic cell culture experiments. If you are interested, please contact us at contact@elveflow.com
- Bubble Trap: add it to remove bubbles from your microfluidic setup
- Additional reservoirs
- Additional flow rate sensors (consult the MFS and BFS sensor product page for more specification details)
- Computer
- Microscope and camera
Videos
Microfluidics related webinars
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